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Effects of elevated CO<sub>2</sub> on growth of Pinus densiflora seedling and enzyme activities in soil
Effects of elevated CO2 on growth of Pinus densiflora seedling and enzyme activities in soil
Journal of Ecology and Environment. 2010. Jun, 33(2): 133-139
Copyright ©2010, The Ecological Society of Korea
This is an Open Access article distributed under the terms of theCreative Commons Attribution Non-Commercial License(http://creativecommons.org/licenses/by-nc/3.0/)which permits unrestrictednon-commercial use, distribution, and reproduction in any medium,provided the original work is properly cited.
  • Received : November 17, 2009
  • Published : June 01, 2010
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About the Authors
Sung-Hyun Kim
Division of EcoScience, Ewha Womans University, Seoul 120-750, Korea
Soo-Hyun Jung
Department of Environmental Science and Engineering, Ewha Womans University, Seoul 120-750, Korea
Ho-Jung Kang
Department of Civil and Environmental Engineering, Yonsei University, Seoul 120-750, Korea
In-Sook Lee
Division of EcoScience, Ewha Womans University, Seoul 120-750, Korea
islee@ewha.ac.kr

Abstract
Atmospheric CO 2 concentrations have increased exponentially over the last century and, if continued, are expected to have significant effects on plants and soil. In this study, we investigated the effects of elevated CO 2 on the growth of Pinus densiflora seedling and microbial activity in soil. Three-year-old pine seedlings were exposed to ambient as well as elevated levels of CO 2 (380 and 760 ppmv, respectively). Growth rates and C:N ratios of the pine seedlings were also determined. Dissolved organic carbon content, phenolic compound content, and microbial activity were measured in bulk soil and rhizosphere soil. The results show that elevated CO 2 significantly increased the root dry weight of pine seedling. In addition, overall N content decreased, which increased the C:N ratio in pine needles. Elevated CO 2 decreased soil moisture, nitrate concentration, and the concentration of soil phenolic compounds. In contrast, soil enzymatic activities were increased in rhizosphere soil, including β-glucosidase, N-acetylglucosaminidase and phosphatase enzyme activities. In conclusion, elevated CO 2 concentrations caused distinct changes in soil chemistry and microbiology.
Keywords
INTRODUCTION
At their current rate of increase, CO 2 levels are expected to double to 750 ppm by the end of this century. Elevated CO 2 significantly influences both soil nutrient availability and soil microbes that are associated plants (Janus et al. 2005). Numerous studies that have investigated the effects of elevated CO 2 on plants found that elevated CO 2 increases the growth rate of plants (Gifford 1994, Naidu et al. 1998) while significantly affecting physiological function (Melillo et al. 1990). In addition, elevated atmospheric CO 2 causes an increase in the C:N ratios of plants by reducing the N concentration (Berntson and Bazzaz 1996). Such results were due to changes in the level of Rubisco or respiratory proteins, or to the dilution of nitrogen resulting from the accumulation of non-structural carbohydrates. Sims et al. (1998) recently reported that nitrogen levels are higher in plants under elevated CO 2 . However, Saxe et al. (1998) found that elevated CO 2 had no effect on the nitrogen or carbon content of oak and beech seedlings. These results suggest the level of nitrogen in plant tissue exposed to elevated CO 2 is species-dependent.
Enzymatic changes under elevated CO 2 can alter the microbial demand for N and therefore the flow of N between soil microorganisms and plant roots (Zak et al. 2000). This in turn alters the overall chemical composition of plants as well as the types of organic substrates available for microbial metabolism (Finzi et al. 2006). Elevated CO 2 can also increase microbial activity in the soil (Hungate et al. 1996) mainly by providing extra C sources for rhizospheric microorganisms (Zak et al. 2000). Soil microbial activity can be measured in terms of CO 2 production or based on the activities of major metabolic enzymes (Klose et al. 2003).
The responses of plants and microorganisms to elevated CO 2 vary according to the plant species (Cotrufo et al. 1998). While much information is available on the effect of elevated CO 2 on trees, little is known about how pine seedlings are affected. Since pine trees are a dominant tree species in eastern Asia, we believe that such information would be highly valuable.
The purpose of this study was to investigate the growth of pine seedling as well as soil microbial activity in response to elevated CO 2 in a growth chamber. We report on the impact of increased CO 2 on the root and shoot growth, biomass (dry weight) and C:N ratio of Pinus densiflora, emphasizing the interdependency of soil chemistry and microbiology, soil moisture and plant growth.
MATERIALS AND METHODS
- Experimental design
Natural soil was sampled from the pine forest on the Ewha Woman’s University campus in Seoul, Korea. Soil samples (1 kg/pot, diameter 10 cm) were used for the planting of three-year-old pine seedlings ( P. densiflora ), which were obtained from the Korean forest service. The plants were incubated for 12 months in growth chambers (Dasol scientific Co., Hwaseong, Korea) under 380 or 760 ppmv CO 2 . The growth chamber was controlled at 25°C and 60% humidity, then subjected to a 16 h light/8 h dark cycle. The CO 2 concentrations were chosen based on Intergovernmental Panel on Climate Change (IPCC) reports (2007) that found the concentration of CO 2 in the atmosphere during the 21 st century will rise to 700 ppm, which is double the current concentration. Further, 30 mL of water and 20 mL of 1/2 nutrient solution (KNO 3 606.6 6 mg/L, Ca(NO 3 ) 2 ·4H 2 O 944.60 mg/L, NH 4 H 2 PO 4 115.02 mg/L, MgSO 4 492.94 mg/L, FeCl 2 ·7H 2 O 492.94 mg/L, MnCl 2 ·4H 2 O 1.78 mg/L, H3BO3 2.84 mg/L, ZnSO4·7H2O 0.23 mg/L, CuSO 4 ·5H 2 O 0.075 mg/L) were added to the soil one time per week. Every 4 months, all the aboveground and underground parts were harvested. The shoot was separated and the roots were collected from the soil by washing. All plant parts were oven-dried before measuring dry matter at 80°C for 12 hours. Then, the shoot, root dry weight, and C, N content were measured. After incubation for 12 months, dissolved organic carbon (DOC) and enzyme activities were measured after bulk and rhizosphere soil were divided. The bulk soil was that remaining after the roots were picked from the pot. The rhizosphere soil was that which still adhered to the roots after gentle shaking.
- Pine seedling growth and C:N ratio analysis
The pine seedlings were planted in a growth chamber at 25°C and 60% humidity, and were subjected to a 16 h light/8 h dark cycle. Shoot, root length, and biomass (dry-weight) were measured every four months. All tests were performed in triplicate. Percent dry weight of N and C content were estimated from leaf and root powder using a Flash EA 1112 Analyzer (Thermo Electron Corporation, Waltham, MA, USA).
- Soil characteristics
Soil pH was determined by adding soil to water at a ratio of 1:5 (w:v). Soil moisture was determined gravimetrically by drying at 105°C for 24 hours, and organic matter content was determined by loss on ignition at 700°C (MAS 7000 oven; CEM, Mattews, NC, USA). Soil cation-exchange capacity was determined according to EPA 9081 methods (US Environmental Protection Agency 1986). Soil nitrate (NO 3 -) content was determined by extracting soil with deionized water and then measuring NO 3 - content in the liquid phase using an NO 3 - electrode (Gelderman and Beegle 1998).
- Analysis of dissolved organic carbon and phenolic compounds in soil
To measure the concentration of DOC, soil was added to water at a ratio of 1:10 (w/v) after which the DOC content was measured using a TOC 5000 (Shimadzu Co., Kyoto, Japan) meter. Specific UV absorbance (SUVA) reveals the nature or quality of DOC in a given sample and is used as a surrogate measurement of DOC aromaticity (Chin et al. 1994). SUVA was measured at 254 nm (SUVA 254 ) due to the strong absorption of natural organic matter at this wavelength. This value correlates strongly with the aromatic carbon content of organic matter (Chin et al. 1994). Phenolic compound content was assayed using Folin-Ciocalteau phenol reagent (Box 1983). One milliliter of sample was added to 1.5 mL of Na 2 CO 3 solution (50 g/L). Then, 0.5 mL of Folin-Ciocalteau solution (diluted 1/4 with deionized water; 0.5 N) was added, followed by incubation of the mixture for 2 hours at room temperature. A standard curve was prepared by applying the same chemicals to a series of 0 to 2 mg/L phenol solutions.The color change of reactants was measured spectrophotometricallyat 750 nm. Once out of range of the standard curve, the samples were diluted with distilled water and the procedure was repeated. Phenol oxidase activity was determined using 10 mM L-dihydroxyphenylalaninesolution as a substrate, according to Pind et al. (1994).
- Analysis of microbial activity in soil
The activities of four extracellular enzymes (β-glucosid ase, N-acetylglucosaminidase, phosphatase, and arylsulfatase) were measured by the MUF-substrate method (Freeman et al. 1996). The concentrations of the MUF-β-glucoside, MUF-N-acetylglucosamine, and MUF-arylsulfate substrate solutions were 400 μM (Sigma, St. Louis, MO, USA) while the concentration of the MUF-phosphate substrate solution was 800 μM (Sigma). Enzyme activities in a slurry containing soil and substrate solution (1:5 w/v) were measured using a fluorimeter. Dehydrogenase activity was measured by 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride (INT) assay (Tabatabai 1982). Mixtures of soil (3 g fresh soil) and substrate solution were incubated for 24 hours at 37℃ after which the reaction products were detected using a spectrophotometer (DR/3000 Spectrophotometer; HACH, Mount Holly, NJ, USA) at 485 nm.
- Statistical analysis
Data were analyzed by one-way ANOVA using SPSS ver. 9.0 (SPSS Inc., Chicago, IL, USA). Tukey’s test after one-way ANOVA was used to determine significance differences in soil parameters and soil enzyme activities in each sample. Growth, biomass, DOC, SUVA, phenolic compounds and phenol oxidase activity were tested for significance by a t-test between ambient and elevated CO 2 (P < 0.05).
Carbon concentration, Nitrogen concentration, and the C:N ratio ofPinus densifloragrown under ambient or elevated atmospheric CO2
Lager Image
Carbon concentration, Nitrogen concentration, and the C:N ratio of Pinus densiflora grown under ambient or elevated atmospheric CO2
RESULTS
- The effects of elevated CO2on the biomass and C:N ratios of pine
Although root and shoot elongation were not significantly affected by elevated CO 2 , the root dry weight of pine under elevated CO 2 was increased ( P < 0.05) (Fig. 1) . C:N ratios of pine needles were increased under elevated CO 2 (Table 1) as well. However, no difference in C:N ratios were observed in roots.
- Comparison of soil physical and chemical characteristics
The physicochemical parameters of the soils are listed in Table 2 . Under elevated CO 2 , both NO 3 - and water content were decreased in soil. The soil pH was found to be mildly acidic (6.3-7.1) while the organic matter content ranged from 2.3 g/kg to 3.5 g/kg.
- Various levels of dissolved organic carbon, aromatic and phenolic compounds in soil
Elevated CO 2 had no effect on DOC concentration in soil (Fig. 2a) . However, the composition of DOC did appear to be influenced. Under elevated CO 2 , the proportion of aromatic material in DOC (estimated by SUVA 254 ) was increased in bulk soil but decreased in rhizosphere soil (Fig. 2b) . In contrast, phenolic compound content was decreased in both rhizosphere and bulk soil (Fig. 2c) . Interestingly, phenol oxidase activity was decreased only in rhizosphere soil (Fig. 2d) .
Lager Image
Root, shoot length and biomass of pine seedling under ambient and elevated CO2. (a) Root length, (b) shoot length, and (c) biomass. C, CO2 380 ppmv; E, CO2 760 ppmv; 4M, after 4 months; 8M, after 8 months; 12M, after 12 months.
Lager Image
(a) Dissolved organic carbon concentration, (b) specific UV absorbance (SUVA254), (c) phenolic compounds, and (d) phenol oxidase activity after 12 months. C, 380 ppmv, bulk soil; CR, 380 ppmv, rhizosphere soil; E, 760 ppmv, bulk soil; ER, 760 ppmv, rhizosphere soil.
- Soil enzyme activity
Elevated CO 2 increased dehydrogenase activity (a measure of microbial intracellular activity) in bulk soil only (Table 3) . β-glucosidase, N-acetylglucosaminidase, and phosphatase activities under elevated CO 2 were increased in soils, especially rhizosphere soil (Table 3) .
DISCUSSION
Elevated CO 2 affected the C:N ratio of pine seedling as well as soil enzyme activity and N content. However, plant growth and soil DOC content were unaffected. Interestingly, elevated CO 2 increased the C:N ratio of plants as well as microbial activities. It has been suggested that increased CO 2 decreases the level of nitrate in soil. Elevated CO 2 could likely decrease N into the soil, both of which are necessary for plant and microbial decomposition function. These decreases in soil nitrate levels are extremely important because net primary productivity is nitrogen dependent (Janus et al. 2005). Thus, as the amount of available N for plant uptake is decreased, the C:N ratios in pine needles are increased. Gifford et al. (2000) also similarly reported that the C:N ratio of pine seedling is increased 15% due to a 21% decrease in the N content of needles under elevated CO 2 .
Soil physical and chemical parameters
Lager Image
Soil physical and chemical parameters
Enzyme activities in soil under ambient and elevated CO2
Lager Image
Enzyme activities in soil under ambient and elevated CO2
It was observed that the root dry weight of pine seedling was increased under elevated CO 2 , although root and shoot elongation were unaffected (Fig. 1) . Many studies show that the overall biomass is more affected than either root or shoot growth under elevated CO 2 . Pushnik et al. (1999) reported that the root biomass of Pinus ponderosa is significantly increased at 500 and 700 ppmv CO 2 . Further, King et al (2001) reported that the root biomass of pine is increased by 96% under elevated CO 2 . It has been hypothesized that elevated CO 2 will increase biomass partitioning to fine roots (Curtis et al. 1994), thereby increasing the total root surface area. The increase in root biomass would be expected to favor the growth of microbial fungi in soil due to altered soil chemical composition.
The microbial activities of β-glucosidase, N-acetylglucosaminidase, and phosphatase were increased under elevated CO 2 (Table 2) , particularly in rhizosphere soil ( P < 0.05). These increases in enzyme activities may be related to an increase in root exudates and rhizosphere microbe activity (Fig. 1) . Several rhizospheric bacterial species are known to produce compounds such as phytohormones, antifungal molecules or siderophores that assist the plant through atmospheric nitrogen fixation (Rillig et al. 1997). Increases in the activities of N-acetylglucosaminidase and phosphatase often occur in response to nitrogen (Gifford et al. 2000). It has been shown that under elevated CO 2 , β-glucosidase releases more C from organic matter into soil (Larson et al. 2002, Henry et al. 2005) and that both C and N in general, which are important for microbial metabolism, are released.
Elevated CO 2 did not influence the concentration of dissolved organic matter in soil, but the concentrations of phenolic compounds in soil and aromatic compounds in rhizosphere soil were decreased (Fig. 2) . Phenolic compounds are resistant to the nitrification of microbial decomposition activity. Therefore, elevated CO 2 could promote their use as carbon sources by microbes (Rouhier and Read 1998). This notion is supported by our finding that phenol oxidase activity in soil was reduced under elevated CO 2 .
In this study, changes in the level of nitrogen in soil could explain the effects of elevated CO 2 on microbial activities and pine seedling growth. However, as our work is limited to the growth chamber, further investigation is needed study.
The results of this study demonstrated that elevated CO 2 had significant effects on the growth of pine seedling as well as soil microbial activity. These findings suggest that rising levels of atmospheric CO 2 cause a reduction in pine seedling biomass as well as distinct changes in soil chemistry and microbiology.
Acknowledgements
This study was conducted with the support of the Forest Science & Technology Projects (S120507L0201104), provided by Korea Forest Service, and the 2008 KOSEF-DAAD Summer Institute Program, provided by Korea Science and Engineering Foundation.
References
Berntson G.M , Bazzaz F.A 1996 Belowground positive and negative feedbacks on CO2growth enhancement Plant Soil 187 119 - 131
Box J.D 1983 Investigation of the Folin-Ciocalteau Phenol reagent for the determination of polyphenolic substances in natural waters Water Res 17 511 - 525    DOI : 10.1016/0043-1354(83)90111-2
Chin Y.P , Aiken G , O'Loughlin E 1994 Molecular weight polydispersity and spectroscopic properties of aquatic humic substances Environ Sci Technol 28 1853 - 1858    DOI : 10.1021/es00060a015
Cotrufo M.F , Ineson P , Scott A 1998 Elevated CO2reduces the nitrogen concentration of plant tissues Global Change Biol 4 43 - 54    DOI : 10.1046/j.1365-2486.1998101.x
Curtis P.S , O'Neill E.G , Teeri J.A , Zak D.R , Pregitzer K.S 1994 Belowground responses to rising atmospheric CO2: implications for plants soil biota and ecosystem processes Plant Soil 165 1 - 6    DOI : 10.1007/BF00009957
Finzi A.C , Moore D.J.P , DeLucia E.H , Lichter J , Hofmockel K.S , Jackson R.B , Kim H.S , Matamala R , McCarthy H.R , Oren R , Pippen J.S , Schlesinger W.H 2006 Progressive nitrogen limitation of ecosystem processes under elevated CO2in a warm-temperate forest Ecology 87 15 - 25    DOI : 10.1890/04-1748
Freeman C , Liska G , Ostle N.J , Lock M.A , Reynolds B , Hudson J 1996 Microbial activity and enzymic decomposition processes following peatland water table drawdown Plant Soil 180 121 - 127    DOI : 10.1007/BF00015418
Gelderman R.H , Beegle D , Brown J.R 1998 Nitrate-nitrogen In Recommended Chemical Soil Test Procedures for the North Central Region University of Missouri-Columbia Columbia MO 17 - 20
Gifford R.M 1994 The global carbon cycle: a viewpoint on the missing sink Aust J Plant Physiol 21 1 - 15    DOI : 10.1071/PP9940001
Gifford R.M , Barrett D.J , Lutze J.L 2000 The effects of elevated [CO2] on the C : N and C : P mass ratios of plant tissues Plant Soil 224 1 - 14    DOI : 10.1023/A:1004790612630
Henry H.A.L , Juarez J.D , Field C.B , Vitousek P.M 2005 Interactive effects of elevated CO2N deposition and climate change on extracellular enzyme activity and soil density fractionation in a California annual grassland Global Change Biol 11 1808 - 1815    DOI : 10.1111/j.1365-2486.20051007.x
Hungate B.A , Jackson R.B , Field C.B , Chapin F.S 1996 Detecting changes in soil carbon in CO2enrichment experiments Plant Soil 187 135 - 145
Intergovernmental Panel on Climate Change 2007 The Physical Science Basis: Contribution of Working Group I to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change Intergovernmental Panel on Climate Change Intergovernmental Panel on Climate Change Cambridge University Press Cambridge
Janus L.R , Angeloni N.L , McCormack J , Rier S.T , Tuchman N.C , Kelly J.J 2005 Elevated atmospheric CO2alters soil microbial communities associated with trembling aspen (Populus tremuloides) Microb Ecol 50 102 - 109    DOI : 10.1007/s00248-004-0120-9
King J.W , Mohamed A , Taylor S.L , Mebrahtu T , Paul C 2001 Supercritical fluid extraction of Vernonia galmensis seeds Ind Crops Prod 14 241 - 249    DOI : 10.1016/S0926-6690(01)00089-9
Klose S , Wernecke K.D , Makeschin F 2003 Microbial biomass and enzyme activities in coniferous forest soils as affected by lignite-derived deposition Biol Fertil Soils 38 32 - 44    DOI : 10.1007/s00374-003-0615-4
Larson J.L , Zak D.R , Sinsabaugh R.L 2002 Extracellular enzyme activity beneath temperate trees growing under elevated carbon dioxide and ozone Soil Sci Soc Am J 66 1848 - 1856
Rillig M.C , Scow K.M , Klironomos J.N , Allen M.F 1997 Microbial carbon-substrate utilization in the Rhizosphere of Gutierrezia Sarothrae grown in elevated atmospheric carbon dioxide Soil Biol Biochem 29 1387 - 1394    DOI : 10.1016/S0038-0717(97)00053-9
Melillo J.M , Callaghan T.V , Woodward F.I , Salati E , Sinha S.K , Houghton J.T , Jenkins G.J , Ephraums J.J 1990 Effects on ecosystems In Climate Change Cambridge University Press Cambridge 285 - 310
Naidu S.L , DeLucia E.H , Thomas R.B 1998 Contrasting patterns of biomass allocation in dominant and suppressed loblolly pine Can J For Res 28 1116 - 1124    DOI : 10.1139/cjfr-28-8-1116
Pind A , Freeman C , Lock M.A 1994 Enzymic degradation of phenolic materials in peatlands: measurement of phenol oxidase activity Plant Soil 159 227 - 231    DOI : 10.1007/BF00009285
Pushnik J.C , Garcia-Ibilcieta D , Bauer S , Anderson P.D , Bell J , Houpis J.L.J 1999 Biochemical responses and altered genetic expression patterns in ponderosa Pine (Pinus ponderosa Doug ex P Laws) grown under elevated CO2 Water Air Soil Pollut 116 413 - 422    DOI : 10.1023/A:1005207112589
Rouhier H , Read D.J 1998 Plant and fungal responses to elevated atmospheric carbon dioxide in mycorrhizal seedling of Pinus sylvestris Environ Exp Bot 40 237 - 246    DOI : 10.1016/S0098-8472(98)00039-2
Saxe H , Ellsworth D.S , Heath J 1998 Tree and forest functioning in an enriched CO2atmosphere New Phytol 139 395 - 436    DOI : 10.1046/j.1469-8137.1998221.x
Sims D.A , Luo Y , Seemann J.R 1998 Comparison of photosynthetic acclimation to elevated CO2and limited nitrogen supply in soybean Plant Cell Environ 21 945 - 952    DOI : 10.1046/j.1365-3040.1998334.x
Tabatabai M.A , Page A.L 1982 Soil enzymes In Methods of Soil Analysis Part 2 Chemical and Microbiological Properties Agronomy Monograph American Society of Agronomy Madison WI 903 - 904
US Environmental Protection Agency 1986 Test Methods for Evaluating Solid Waste SW-846 Method 9081 US Environmental Protection Agency Washington DC
Zak D.R , Pregitzer K.S , King J.S , Holmes W.E 2000 Elevated atmospheric CO2fine roots and the response of soil microorganisms: a review and hypothesis New Phytol 147 201 - 222    DOI : 10.1046/j.1469-8137.2000687.x