본 연구는 안정 시 고지방식이 흰쥐의 골격근에서 PPAR-δ, PPAR-γ 그리고 PGC-1α 단백질 발현에 손바닥선인장 보충이 미치는 효과에 대하여 연구하였다. SD계 수컷 흰쥐 16마리를 무작위로 대조군(CG, n=8)과 실험군(EG, n=8)으로 분류하였다. 8주 동안 대조군은 고지방식이를 부하하였으며, 실험군은 5% 손바닥선인장을 보충식이하였다. 본 실험결과, 복부지방과 고환부 지방 중량은 EG군이 CG군에 비해 유의하게 낮게 나타났다( p <0.01). 또한 혈당, 중성지방, 총콜레스테롤의 농도도 EG군이 CG군에 비해 유의하게 낮게 나타났다( p <0.01). 한편, 골격근에서 PPAR-γ와 PGC-1α 단백질 발현은 EG군이 CG군에 비해 유의하게 높게 나타났다( p <0.05). 이상의 결과로부터 손바닥선인장 보충이 고지방식이 흰쥐의 혈당과 중성지방 농도의 감소와 골격근에서 PPAR-γ와 PGC-1α 단백질 발현을 증가시킴으로서 체지방을 감소시켜 체중증가 억제에 긍정적인 영향을 미치는 것으로 나타났다. Peroxisome proliferator-activated receptors (PPARs), which are nuclear hormone receptors that activate lipid metabolism pathways such as lipogenesis and lipolysis, are well known to play an important role in maintaining cholesterol and lipid homeostasis. PPARs also regulate the genes involved in the metabolic regulation of lipoproteins, the activity of insulin and the differentiation of adipocytes [6] . PPAR-δ is expressed in various tissues, particularly in muscle tissue, and is involved in the modulation of lipid catabolism and energy uncoupling in skeletal muscles [5] . In addition, PPAR-γ regulates fat storage and adipocyte metabolism [27] , and it has been reported that PPAR-γ is involved in modulating insulin sensitivity in the body [26] . Furthermore, PGC-1α, which is a transcriptional coactivator associated with PPARs expression in tissues, is a regulator of mitochondria biogenesis and lipid metabolism activation [ 2 , 12 ]. In particular, it has been reported that PGC-1α is highly expressed in brown adipose tissue, which is known to have a high capability for oxidation as well as activated transcription of PPAR-γ [ 20 , 29 ]. In a previous study that related the reduction of fat weight and PPARs, Wang et al. [32] reported that activation of PPAR-δ decreased fat mass due to an increase in fatty acid oxidation and utilization in adipocytes and muscle tissues, and it has been reported that reduced expression of PPAR-γ protein in white adipose tissue affected body weight and reduced fat weight [30] . However, although the expression of PPAR-γ is low in muscle, previous studies have suggested that lipid metabolism is related to PPAR-γ protein expression in muscle tissue. Norris et al. [22] reported that body weight significantly increased in PPAR-γ knockout mouse muscle tissue, in contrast with was observed in adipocytes. Nevertheless, although the protein expression of PPAR-γ and PGC-1α was increased in rat muscle tissue, body and fat weight was not affected. Therefore, PPAR proteins are expressed according to tissue-specific patterns. Recently, many studies and development processes have focused on functional food, which can safely be ingested without toxicity as a long-term treatment, to activate lipid metabolism. Approximately 4,000 types of cacti exist in the world, and as a plant that is highly adaptable to semi-arid areas, some varieties have been used as a source of carbohydrates and various vitamins. Particularly used in South Korea, O. humifusa , a tropical plants containing fruits on the stems, is a member of Cactaceae family that has been cultivated to grow in cold environment below −20℃ [8] . O. humifusa contains not only minerals, such as calcium, magnesium, zinc, and ferrum but also soluble fiber, vitamin- C, vitamin- E, flavonoids, and polyphenols. It has been reported that these nutritional compounds play a predominant role in the physiological function of the body [10] . In a previous study, Dok-go et al. [4] described that supplementation with O. ficus- indica , which is repleted with flavonoids such as quercetin that act as antioxidants, exhibited anti-inflammatory effects and neuroprotective effects against oxidative injuries induced in cortical cell cultures in rats. It has been reported that cactus extract supplementation protects the gastric mucous membrane and suppresses carrageenan-induced edema and beta-glucuronidase and lysosomal enzyme secretion in neutrophils [24] . Although various research has been performed that reported that O. humifusa supplementation has a positive effect on the physiological function to the body, there have not been any studies designed to evaluate the protein expression related to fatty acids regulation by O. humifusa . Therefore, in this study, we investigated the effects of O. humifusa supplementation on body weight and changes in fat weight as well as on PPAR-γ, δ and PGC-1α protein expression, which plays an important role in transcriptional gene regulation of fatty acid metabolism in muscle tissue, in high-fat diet-induced rats. All experimental protocols were approved by the Animal Study Committee of Sunmoon University. After the acclimatization period during week one, sixteen 6-week-old male Sprague-Dawley rats (Samtaco Bio Korea, Hwaseong, Korea) were randomly divided into two groups: a control diet group (CG: high-fat diet group, n=8) and an experimental diet group (EG: 5％ O. humifusa supplemented diet group, n=8), given free access to tap water and food for 8 weeks, and housed in groups of two per cage under controlled temperature (23±1℃) and relative humidity (50±5％). The light/dark cycle was automatically controlled (alternating 12-h periods), and lighting began at 8:00 pm. Food intake was measured daily and body weight was measured weekly. At the end of the experimental period, the rats were anesthetized with diethyl ether after fasting for 12 h. Blood samples were taken from the left ventricle and serum was obtained by centrifuging the blood at 700× g for 20 min at 4℃. Both hind limb muscles were dissected and immediately immersed in liquid nitrogen. The serum samples and hind limb muscles were stored at −70℃ until analyzed. O. humifusa , which was harvested in Asan, Chungnam, was cleaned and blended using a HMF-3150S blender (Hanil Electronics, Seoul, Korea). After blending, the O. humifusa was frozen in a freezer at a temperature of −70℃ and then freeze-dried in a freeze dryer (Ilshin Co., Gyeonggi, Korea). After freeze-drying, as shown in Table 1 , a general component analysis of O. humifusa was performed using the Association of Official Analytical Chemists (AOAC) method for the following measurements: moisture, using an air-oven method; ash, using a dry-ashing method; carbohydrates, using calculation; crude protein, using a Keldahl method; crude fat, using a Soxhlet extraction method; and fiber, using an enzymatic-chemical method. Mineral component analysis was performed using plasma atomic emission spectrometry (ICP-AES) to determine the composition of Fe ²⁺ , Ca ²⁺ , Mg ²⁺ , K ⁺ , Na ⁺ , and P ²⁺ . As shown in Table 2 , the high-fat diet was composed of 20％ protein, 48％ carbohydrate and 20% fat and was modified from a previous study [18] and based on AIN-76G. The 5％ O. humifusa diet was made by substituting a portion of carbohydrates, protein, fiber, and fat components of the high-fat diet. During the experimental period, the diet was prepared in batches sufficient for 3-5 days, and the experimental diets were stored at 4℃ to maintain freshness. Serum glucose, TG, TC, and HDLC levels were analyzed using enzymatic kits (Asan Pharmaceutical Co, Yongin, Korea). For protein expression analysis, soleus muscle was homogenized on ice with a polytron homogenizer in 20 mmol/l Tris-HCl buffer (pH 7.5) containing 5 mmol/l EDTA, 2 mmol/l PMSF, and 1:200 protease inhibitor cocktail (Sigma, St Louis, MO, USA). The protein concentrations were determined using Bradford reagent (Bio-Rad, Hercules, CA, USA), with bovine serum albumin as the standard. An aliquot of tissue extract containing 20 µg of protein (for PPAR-δ, PPAR-γ and PGC-1α) was separated on a 10％ SDS-PAGE gel. After electrophoresis, the proteins were transferred to a PVDF membrane (Millipore, Bedford, MA, USA) using a semi-dry blotting apparatus (Bio-Rad, Hercules, CA, USA). After treating with blocking buffer (PBS containing 10％ skim milk) for 90 min, the membrane was incubated with primary polyclonal antibodies for 2 h (PPAR-δ, PPAR-γ and PGC-1α; Santa Cruz Biotechnology, CA, USA), followed by five 10 min. washes with PBS (5％ Tween-20). The membrane was then incubated with HRP-conjugated anti-goat IgG or anti-rabbit IgG (Santa Cruz Biotechnology, CA, USA) for 1 h, followed by five 10 min. washes with PBS (5％ Tween-20). The target proteins were detected using an ECL kit (Amersham Pharmacia Biotech, Piscataway, NJ, USA). The films were photographed and the protein bands of interest were quantified using band analyzer software (Bio-Rad, Hercules, CA, USA). All data were analyzed using SPSS software (version 16.0 for Windows). The data are expressed as the mean ± SE, and values were analyzed with the independent samples t test. Significance was defined as p ＜0.05. In order to determine the effect of O. humifusa supplementation on the body weight gain and fat tissue weights, both body weight and fat tissue weights were measured by using a digital electronic balance. As shown in Table 3 , body weight gain was slightly lower in the EG compared with the CG after 8 weeks of O. humifusa supplementation. However, the abdominal fat pad and epididymal fat pad were significantly smaller in the EG than in the CG ( p ＜0.05). The enzymatic kits were used to estimate the effect of O. humifusa supplementation on serum parameters such as glucose, TG, HDLC, and TC. After the 8 weeks of the experiment, as shown in Table 4 , the glucose, TG and TC concentrations of the EG were significantly lower than those of the CG ( p ＜0.01). Additionally, the HDLC concentration of the EG tended to be higher than that of the CG, but the difference was not significant. Western blot analysis was performed to analyze the PPAR- δ, PPAR-γ, and PGC-1α protein expressions using homogenated soleus muscles. As shown in Fig. 1A , the expression of PPAR-δ protein in the EG tended to be higher than in the CG, but this difference was not significant. However, PPAR-γ was significantly higher in the EG than in the CG ( p ＜0.05, Fig. 1B ). As shown in Fig. 2 , PGC-1α protein expression in the EG was significantly higher than in the CG ( p ＜0.05). This study investigated the effect of O. humifusa supplementation on body weight and changes in fat weight as well as PPAR- δ, γ and PGC-1α protein expression on fatty acid metabolism in muscle tissue. In the present study, body weight was not significantly different between groups, whereas the PFT and EFT of the EG were significantly decreased compared with the CG. Increases in calcium intake, one of the major components of O. humifusa , have been shown to improve fatty acid oxidation by suppressing fatty acid synthase and thus reducing the intra-adipocyte calcium level through decrease parathyroid hormone secretion and reduced fat accumulation as well as increased lipolysis [36] . In a previous study, Teegarden et al. [31] reported that the rate of fat oxidation was significantly increased after 12 weeks of a 1,400 mg/day calcium-supplemented diet in obese women aged 18-31 compared with controls. We analyzed the nutritional composition of freeze-dried O. humifusa in the present study and found that, it contains 2,300 mg/100 g of calcium. In addition, we calculated that the experimental diet group received approximately 1.3 times more calcium than the control group due to the 5％ O. humifusa supplementation. In turn, fat oxidation of PFT and EFT in the EG was increased, which suppressed fat accumulation and increased lipolysis in the fat tissue of the EG, similar to what was observed in the previous study. Furthermore, we suggest that in the previous studies, increased expression of PPAR-δ, PPAR-γ, and PGC-1α proteins activated fatty acid metabolism [ 16 , 32 ], suggesting that increased PPAR-γ and PGC-1α may have influenced fat weight reduction in the present study. Serum analysis showed that serum glucose, TG, and TC of the EG were significantly decreased compared with the CG, and HDLC tended to increase in the EG compared with the Bwititi et al. [3] reported that orally humifusa extract supplementation (20 mg/ 100 g body weight) lowers serum glucose levels through an insulin-independent mechanism in streptozotocin (STZ)-diabetic rats, and the glucose lowering effect was due to an increase in intracellular glucose uptake through the renal reabsorption of Na ⁺ after extract supplementation. In addition, it has been reported that insulin resistance was improved because TNF-α, which is a cytokine that is secreted from adipocytes and causes insulin resistance, is reduced by PPAR-γ agonists [21] . Therefore, increased PPAR-γ caused a reduction in glucose level in the present study. Meanwhile, it has been reported that increased lipoproteins such as TC, TG and LDLC cause hyperlipidemia during high-fat diet supplementation in a rat model [ 13 , 35 ]. However, fiber, which is also a component of O. humifusa , improved hyperlipidemia through by suppressing endogenous cholesterol synthesis by hindering intestinal fat absorption, increasing bile acid synthesis, and forming fatty acids with a lower number of carbons, which are degraded in the large intestine [ 1 , 28 ]. Similar to our present data, Hahm et al. [11] found that glucose, TG, TC and LDLC were significantly decreased in STZ-induced diabetic rats after oral supplementation with 250 mg/kg/day O. humifusa extract. It has been reported that supplementation with 2.5 g /100 g diet prickly pear pectin, another cactus, has a positive effect on lowering serum lipid profiles in hyperlipidemia- induced guinea pigs [7] , and serum LDLC, TG and TC levels are decreased by O. robusta administration in hyperlipidemic middle-aged men [33] . As a result, we might assume that the reduction in serum TG and TC levels in the EG is caused by an improvement in hepatic cholesterol metabolism due to increased intake of fiber and pectin with the O. humifusa -supplemented diet. Meanwhile, expression of PPAR-γ and PGC-1α in the muscle in the EG was significantly increased compared with the CG, and PPAR-δ in the EG tended to increase in muscle tissue compared with the control group. Although PPAR-δ protein is expressed in various tissues, its expression is highest in muscle tissue [14] , and it is involved in the catabolism of lipids as well as the regulation of energy uncoupling in skeletal cells [5] . Wang et al. reported that the activation of the PPAR-δ protein in adipocyte and skeletal muscle increased fat oxidation and utilization in an in vivo study using obese Zucker rats. Therefore, we suggest that O. humifusa supplementation partially affected fatty acid metabolism in muscles because an increasing tendency of PPAR-δ protein expression was observed after O. humifusa supplementation. Meanwhile, although PPAR-γ protein is highly expressed in adipose tissue, it is involved in fat oxidation-related gene expression in skeletal muscle tissue [19] and also plays an important role in lipid metabolism [9] . Similar to our present study, Kang et al. [17] reported that 8 weeks of O. humifusa supplementation increased PPAR-γ protein expression in a rat model. We suggest that increased PPAR-γ protein expression in the present study occurred through increased PGC-1α, which is a co-activator of PPAR-γ, due to intake of calcium-rich O. humifusa . PGC-1α lead to PPAR expression as a co-activator and simulated mitochondrial biogenesis in muscle tissue as well as increased fatty acid oxidation through a transfer to slow twitch muscle fiber, which is favorable for fatty acid oxidation metabolism [25] . Calcium, in which O. humifusa is rich, plays an important role in the modulation of the intracellular signaling second messenger that leads to muscle contraction and gene expression. Increased calcium intake is highly involved in improving lipid metabolism, oxidative phosphorylation, mitochondrial biogenesis, and PGC-1α protein expression due to an increase in the activation of AMP-activated protein kinase (AMPK) and calcium/calmodulin-dependent protein kinase (CAMK) [ 15 , 23 , 34 ]. As a result, we suggest that the intake of calcium-rich O. humifusa increases the activation of intramuscular AMPK and CAMK by increasing the serum calcium level, resulting in the expression of PPAR-γ and PGC-1α, which are involved in lipid metabolism. Furthermore, as shown in a previous study, our results indicated that a reduction in fat weight, an improvement in serum lipid profiles, and a lowering of serum glucose level due to improvement of insulin sensitivity are caused by increased muscular PPAR-γ and PGC-1α protein expression, which lead to the activation of fatty acid metabolism. These results show that supplementation with O. humifusa for 8 weeks plays a positive role in lowering serum glucose and TG levels and suppressing weight gain by reducing fat weight through increasing the expression levels of PPAR-γ and PGC-1α proteins in the muscle tissue of rats.