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Cloning of Chondroitinase ABC from Bacteroides stercoris HJ-15, a Human Intestinal Anaerobic Bacterium
Cloning of Chondroitinase ABC from Bacteroides stercoris HJ-15, a Human Intestinal Anaerobic Bacterium
Microbiology and Biotechnology Letters. 2016. Jun, 44(2): 140-144
Copyright © 2016, The Korean Society for Microbiology and Biotechnology
  • Received : December 16, 2015
  • Accepted : February 28, 2016
  • Published : June 28, 2016
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서현, 방
주원, 심
양진, 현
동현, 김
dhkim@khu.ac.kr

Abstract
The gene encoding chondroitinase ABC from a genomic library of Bacteroides stercoris HJ-15, which was isolated from human feces, was cloned. The cloned gene consisted of 3,090 bp and was predicted to encode a 1,029−amino-acid protein. The B. stercoris chondroitinase ABC gene was not homologous to other chondroitinase ABC genes; however, its amino acid sequence showed 71% homology to that of Bacteroides thetaiotaomicron . The gene was cloned in the pET-26b+ expression vector and expressed under the T7 promoter in Escherichia coli BL21(DE3). The purified recombinant chondroitinase ABC degraded chondroitin sulfates A, B, and C.
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