Advanced
Development of a Diagnostic System for the Detection of the Cowpea mild mottle virus Specific Gene in Quarantine
Development of a Diagnostic System for the Detection of the Cowpea mild mottle virus Specific Gene in Quarantine
Microbiology and Biotechnology Letters. 2015. Sep, 43(3): 296-299
Copyright © 2015, The Korean Society for Microbiology and Biotechnology
  • Received : July 20, 2015
  • Accepted : August 29, 2015
  • Published : September 28, 2015
Download
PDF
e-PUB
PubReader
PPT
Export by style
Share
Article
Author
Metrics
Cited by
TagCloud
About the Authors
시원 이
진영 이
보영 문
창수 김
용길 신
재영 노
jyrho@dankook.ac.kr

Abstract
Cowpea mild mottle virus (CPMMV)는 국내 미보고 바이러스로, 넓은 숙주범위를 가지며, 국내 관리급 검역바이러스로 지정되어 있다. 본 연구에서는 검역현장에서 신속하게 CPMMV를 진단할 수 있는 방법을 개발하였다. 이번 연구는 사용자를 위한 검사방법 개발을 위하여, 기존의 검역현장에서 활용하는 PCR 조성과 조건을 활용하였다. 본 연구에서는 CPMMV를 특이적으로 진단할 수 있는 2개의 RT-PCR 프라이머를 개발하였으며 각각 579와 638 bp를 증폭할 수 있다. 최종적으로 증폭되는 nested PCR 산물의 크기는 (579 → 298 bp)과 (638 → 252 bp)로 CPMMV의 특이 유전자를 진단할 수 있다. 또한, 제한효소 Xho I이 반응할 수 있는 염기 서열을 삽입하여 양성대조구 플라스미드를 개발하였다. 이것은 실험실 오염으로부터 거짓양성을 검증할 수 있게 하였다. 본 연구는 CPMMV와 관련된 수입 작물로부터 검역진단에 기여할 것이라고 사료된다.
Keywords
PPT Slide
Lager Image
PPT Slide
Lager Image
PPT Slide
Lager Image
Acknowledgements
The present research was conducted by the Research Fund of Animal and Plant Quarantine Agency in 2006−2008 and by a grant from the Animal and Plant Quarantine Agence (QIA), Ministry of Agriculture, Republic of Korea.
References
2013 List of Plant Quarantine Viruses in Korea in Newly Revised in 2013 Res. Plant Dis. 19 67 - 75
Brunt AA , Kenten RH 1973 Cowpea mild mottle, a newly recognized virus infecting cowpea (Vigna unguiculata) in Ghana Ann. Appl. Biol. 74 67 - 74    DOI : 10.1111/j.1744-7348.1973.tb07723.x
Brunt AA , Phillips S 1981 “Fuzzy vein”, a disease of tomato (Lycopersicon esculentum) in western Nigeria induced by Cowpea mild mottle virus Tropical Agriculture 58 177 - 180
Caruso P , Bertolini E , Ca M , López MM 2003 A new and sensitive co-operational polymerase chain reaction for rapid detection of Ralstonia solanacearum in water J. Microbiol. Methods 55 257 - 272    DOI : 10.1016/S0167-7012(03)00161-1
Jeyanandarajah P , Brunt AA 1993 The natural occurrence, transmission, properties and possible affinities of Cowpea mild mottle virus J. Phytopathol. 137 148 - 156    DOI : 10.1111/j.1439-0434.1993.tb01334.x
Lee S 2013 A study of molecular biological detection methods for seed-transmitted viruses in quarantine. Ph. D. thesis Dankook University Cheonan, Chungcheongnam-do, Korea
Lee S , Kang EH , Chu YM , Shin YG , Ahn TY 2013 Development of PCR diagnosis system for plant quarantine seed-borne Wheat streak mosaic virus Korean J. Microbiol. 49 112 - 117    DOI : 10.7845/kjm.2013.3013
Lee S , Kang EH , Heo NY , Kim SM , Kim YJ , Shin YG 2013 Detection of Carnation necrotic fleck virus and Carnation ringspot virus using RT-PCR Res. Plant Dis. 19 36 - 44    DOI : 10.5423/RPD.2013.19.1.036
Lee S , Kang EH , Shin YG , Lee SH 2013 Development of RT-PCR and nested PCR for detection of four quarantine plant viruses belonging to Nepovirus Res. Plant Dis. 19 220 - 225    DOI : 10.5423/RPD.2013.19.3.220
Lee S , Lee JY , Shin YG , Lee SH , Ahn TY 2015 Development and verification of nested PCR assay for detection of Tobacco rattle virus in plant quarantine J. Bacteriol. Virol. 45 54 - 61    DOI : 10.4167/jbv.2015.45.1.54
Lee S , Rho JY 2015 Development of a PCR diagnostic system for detecting Andean potato mottle virus asociated with potato quarantine in Korea Am. J. Potato Res. In press
Lee S , Shin YG 2014 Development and practical use of RT-PCR for seed-transmitted Prune dwarf virus in quarantine Plant Pathol. J. 30 178 - 182    DOI : 10.5423/PPJ.NT.10.2013.0099
Mink GI , Keswani CL 1987 First report of Cowpea mild mottle virus on bean and mung bean in Tanzania Plant Dis. 71 557 -
Shin YG 2009 An advanced quarantine system for the inspection of seed-borne viruses in Korea. Ph. D. thesis Kyungpook National University Daegu, Gyeongsangbuk-do, Korea
Stein A , Loebensten G , Koenic R 1979 Detection of Cucumber mosaic virus and Bean yellow mosaic virus in gladiolus by enzymelinked immunosobent assay (ELISA) Plant Dis. Rep. 63 185 - 188