Development of a Diagnostic System for the Detection of the Cowpea mild mottle virus Specific Gene in Quarantine
Development of a Diagnostic System for the Detection of the Cowpea mild mottle virus Specific Gene in Quarantine
Microbiology and Biotechnology Letters. 2015. Sep, 43(3): 296-299
Copyright © 2015, The Korean Society for Microbiology and Biotechnology
  • Received : July 20, 2015
  • Accepted : August 29, 2015
  • Published : September 28, 2015
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시원, 이
진영, 이
보영, 문
창수, 김
용길, 신
재영, 노

Cowpea mild mottle virus (CPMMV)는 국내 미보고 바이러스로, 넓은 숙주범위를 가지며, 국내 관리급 검역바이러스로 지정되어 있다. 본 연구에서는 검역현장에서 신속하게 CPMMV를 진단할 수 있는 방법을 개발하였다. 이번 연구는 사용자를 위한 검사방법 개발을 위하여, 기존의 검역현장에서 활용하는 PCR 조성과 조건을 활용하였다. 본 연구에서는 CPMMV를 특이적으로 진단할 수 있는 2개의 RT-PCR 프라이머를 개발하였으며 각각 579와 638 bp를 증폭할 수 있다. 최종적으로 증폭되는 nested PCR 산물의 크기는 (579 → 298 bp)과 (638 → 252 bp)로 CPMMV의 특이 유전자를 진단할 수 있다. 또한, 제한효소 Xho I이 반응할 수 있는 염기 서열을 삽입하여 양성대조구 플라스미드를 개발하였다. 이것은 실험실 오염으로부터 거짓양성을 검증할 수 있게 하였다. 본 연구는 CPMMV와 관련된 수입 작물로부터 검역진단에 기여할 것이라고 사료된다.
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The present research was conducted by the Research Fund of Animal and Plant Quarantine Agency in 2006−2008 and by a grant from the Animal and Plant Quarantine Agence (QIA), Ministry of Agriculture, Republic of Korea.
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