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Anti-Oxidative and Anti-Inflammatory Activities of Cotoneaster horizontalis Decne Extract
Anti-Oxidative and Anti-Inflammatory Activities of Cotoneaster horizontalis Decne Extract
Microbiology and Biotechnology Letters. 2015. Sep, 43(3): 280-285
Copyright © 2015, The Korean Society for Microbiology and Biotechnology
  • Received : July 31, 2015
  • Accepted : September 02, 2015
  • Published : September 28, 2015
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About the Authors
지영 이
경숙 진
현주 권
병우 김
bwkim@deu.ac.kr

Abstract
Cotoneaster horizontalis Decne 에탄올 추출물(CHEE)의 항산화능과 항염증 생리활성을 분석하였다. CHEE의 항산화능을 DPPH radical scavenging activity로 분석한 결과 radical 소거능의 정도가 양성 대조군으로 사용한 ascorbic acid와 유사한 정도의 높은 활성을 보여 매우 강한 항산화능을 보유함을 확인하였다. 또한 RAW 264.7 세포주를 이용하여 H 2 O 2 유도에 의해 생성된 ROS에 대한 소거능을 분석한 결과에서도 강한 소거능을 보였다. 뿐만 아니라 항산화효소 HO-1 및 그 전사 인자인 Nrf2의 단백질 발현이 CHEE의 처리에 의해 증가되었다. 한편 CHEE가 LPS에 의해 유도된 NO 생성에 미치는 영향을 분석한 결과 농도의존적인 NO 생성 저해능을 보였으며 이는 NO 생성 단백질인 iNOS의 발현 저해에서 기인함을 확인하였다. 이러한 결과를 통해 C. horizontalis 의 항산화능과 항염증 활성을 세포 수준에서 처음으로 확인하였으며 향후 기능성 소재로서 유용하게 활용될 수 있을 것으로 판단된다.
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Acknowledgements
This work was supported by Blue-Bio Industry Regional Innovation Center (RIC08-06-07) at Dong-Eui University as a RIC program under Ministry of Trade, Industry and Energy (MOTIE) and Busan city.
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