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Development of Diagnostic PCR System for Three Seedtransmitted Quarantine Viruses Associated with Cucurbitaceae
Development of Diagnostic PCR System for Three Seedtransmitted Quarantine Viruses Associated with Cucurbitaceae
Microbiology and Biotechnology Letters. 2015. Mar, 43(1): 79-83
Copyright © 2015, The Korean Society for Microbiology and Biotechnology
  • Received : October 31, 2014
  • Accepted : December 12, 2014
  • Published : March 28, 2015
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About the Authors
시원 이
병대 민
진영 이
용길 신
수헌 이
suheon@knu.ac.kr
원화 정
suheon@knu.ac.kr

Abstract
The Cucurbitaceae are a plant family that consist of over a hundred genera, the most important of which are squash, pumpkin, zucchini, some gourds, cucumber and watermelon. These are among the top imported seeds in Korea. At the time of their import, the Squash mosaic virus (SqMV), the Cucumber green mottle mosaic virus (CGMMV) and the Kyuri green mottle mosaic virus (KGMMV) are designated as regulated viruses for quarantine in Korea. This study was conducted to develop specific primer sets for easy and rapid detection of SqMV, CGMMV and KGMMV. RT-PCR with the nested PCR primer sets and modified positive control plasmids were capable of highly sensitive detection and verification of such viruses. In addition, developed diagnostic PCR systems applied to quarantine sites detected 47 cases of SqMV, 67 cases of CGMMV and 17 cases of KGMMV between 2010 and the first half of 2014.
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Lane M, 100 bp step DNA Ladder; lane 1, SqMV final selected primer set #4 (680 bp); lane 2, SqMV nested-PCR product from primer set #4 (567 bp); lane 3, SqMV final selected primer set #3 (852 bp); lane 4, SqMV nested-PCR product from primer set #3 (680 bp); lane 5, CGMMV final selected primer set #11 (447 bp); lane 6, CGMMV nested-PCR product from primer set #11 (301 bp); lane 7, CGMMV final selected primer set #14 (609 bp)(Data from Park et al., 2011); lane 8, CGMMV nested-PCR product from primer set #14 (372 bp); lane 9, KGMMV final selected primer set #10 (525 bp); lane 10, KGMMV nested-PCR product from primer set #10 (197 bp); lane 11, KGMMV final selected primer set #12 (780 bp); lane 12, KGMMV nested-PCR product from primer set #12 (430 bp).
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Acknowledgements
The present research was conducted by the Research Fund of Animal and Plant Quarantine Agency in 2006-2008 and by a grant from the Animal and Plant Quarantine Agence (QIA), Ministry of Agriculture, Republic of Korea (Project Code No. Z-1542051-2013- 15-01).
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