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Suppression of the Wnt/β-catenin Pathway by Bryostatin-1
Suppression of the Wnt/β-catenin Pathway by Bryostatin-1
Microbiology and Biotechnology Letters. 2014. Jan, 42(1): 89-92
Copyright © 2014, The Korean Society for Microbiology and Biotechnology
  • Received : February 03, 2014
  • Accepted : February 18, 2014
  • Published : January 28, 2014
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About the Authors
서영 박
상택 오
ohsa@kookmin.ac.kr

Abstract
Wnt/β-catenin 신호전달체계는 세포 증식, 분화, 그리고 기관 발생과 같은 다양한 생명현상에 중요한 역할을 한다. 본 연구에서는 세포기반 스크리닝 기법을 사용하여 Wnt/β-catenin 신호전달체계를 저해하는 bryostatin-1을 발굴하였다. Bryostain 1은 β-catenin의 mRNA 수준에는 영향을 미치지 않는 반면 세포 내 β-catenin 단백질 수준을 감소시킴으로 Wnt3a-CM에 의해 활성화 된 β-catenin response transcription (CRT)을 억제하였다. 또한 프로테아좀의 활성을 저해하였을 경우 bryostatin-1에 의한 β-catenin 수준 감소가 억제되었다. 본 연구의 결과들로부터 bryostatin-1이 프로테아좀에 의한 β-catenin 단백질 분해를 촉진함으로써 Wnt/β-catenin 신호전달체계를 저해함을 확인하였다.
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Acknowledgements
This work was supported by the Basic Science Research Program (2012R1A2A2A01002941) and the Fundamental Technology Program (2012M3A9B2028335) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology.
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