The marine unicellular red algal genus
Rhodella
was established in 1970 by L. V. Evans with a single species
R. macu-lata
based on nuclear projections into the pyrenoid.
Porphyridium violaceum
was described by P. Kornmann in 1965 and transferred to
Rhodella
by W. Wehrmeyer in 1971 based on plastid features and the non-parietal position of the nucleus. Molecular and fine structural evidences have now revealed that
Rhodella maculata
and
R. violacea
are one species, so
R. violacea
has nomenclatural priority and the correct name is
Rhodella violacea
(Kornmann) Wehrmeyer. The status of families within Rhodellophyceae was examined. The order Dixoniellales and family Dixoniellaceae are emended to include only
Dixoniella
and
Neorhodella
. The order Rhodellales and family Rhodellaceae are emended to include
Rho-della
and
Corynoplastis
.
Glaucosphaera vacuolata
Korshikov and the Glaucosphaeraceae Skuja (1954) with an emended description are transferred to the Glaucosphaerales ord. nov.
INTRODUCTION
The genus
Rhodella
was established by Evans (1970) to distinguish a unicellular red alga that possessed con-spicuous ultrastructural features different from the other genera examined by transmission electron microscopy (TEM) at that time,
Porphyridium
(e.g., Gantt and Conti 1965, Gantt et al. 1968) and
Rhodosorus
(Giraud 1962).
Rhodella maculata
(Evans 1970) has highly dissected chloroplast lobes attached to multiple sites of a central to eccentric naked pyrenoid. Pyrenoids are designated as being naked if they are not embedded in the chloro-plast and instead border the cytoplasm, often partially surrounded by starch grains. The pyrenoid of
Rhodella
is unusual among red algal unicells in that the matrix does not contain thylakoids and it is invaded by one or two protrusions from the nucleus (Evans 1970, Patrone et al. 1991, Waller and McFadden 1995, Yokoyama et al. 2004). No other formally identified unicellular red algal genus has both of these subcellular features (Scott et al. 2008, Yang et al. 2010). In Wehrmeyer (1971) used TEM to char-acterize cells previously established as
Porphyridium vio-laceum
(Kornmann 1965) and determined that the cells bore a greater resemblance to
R. maculata
than to
Por-phyridium
. Wehrmeyer transferred
P. violacea
to the ge-nus
Rhodella
as
R. violacea
(Kornmann) Wehrmeyer. The two
Rhodella
species differed slightly from each other. The nuclear protrusions into the pyrenoid of
R. violacea
were not detected by Wehrmeyer (1971) but Patrone et al. (1991) noted their presence. In addition to having unique pyrenoids, both species lack a peripheral thylakoid in the chloroplast and Golgi bodies are associated only with en-doplasmic reticulum (ER) and never with the nucleus or mitochondria (Scott et al. 2008, Yang et al. 2010, 2011).
In the 1980s, two more unicellular red algae were iso-lated, characterized by TEM and assigned to the genus
Rhodella, R. reticulata
(Deason et al. 1983) and
R. cyanea
(Billard and Fresnel 1986). The establishment of these two new algae as presumed species of
Rhodella
was again based on their ultrastructural dissimilarities to
Porphy-ridium
. But what was unexpected and left unexplained was the fact that these algae also did not bear any resem-blance to either
R. maculata
or
R. violacea
. Furthermore,
R. reticulata
and
R. cyanea
clearly bore little resemblance to each other. Subsequent ultrastructural investigations resulted in transferring
R. reticulata
(=
R. grisea
, Fresnel et al. 1989) to a new genus
Dixoniella
as
D. grisea
(Scott et al. 1992) and
R. cyanea
was transferred to a new genus
Neorhodella
as
N. cyanea
(Scott et al. 2008). Recent mo-lecular studies have confirmed the morphological work (Yokoyama et al. 2009) and
R. maculata
and
R. violacea
are currently assigned to the class Rhodellophyceae, Or-der Rhodellales, whereas
D. grisea
and
N. cyanea
are in the class Rhodellophyceae, Order Dixoniellales.
Yoon et al. (2006) established six classes of red algae in subphylum Rhodophytina, three of which include uni-cellular forms, the Porphyridiophyceae (
Erythrolobus, Flintiella, Porphyridium
, and
Timspurckia
), Stylonema-tophyceae (
Rhodosorus
and
Rhodospora
), and Rhodel-lophyceae (
Corynoplastis, Dixoniella, Glaucosphaera, Neorhodella
, and
Rhodella
). For details of the unicellular red algae systematics of utilizing microscopic, molecular, and biochemical methods see Yoon et al. (2006), Scott et al. (2008), Yokoyama et al. (2009), and Yang et al. (2010, 2011).
This current work presents molecular, confocal micros-copy, and electron microscopy details on culture strains of a unicellular red alga identified as
Rhodella
sp. isolated from Bodega Bay, CA and Friday Harbor WA, USA. For the comparison, we added information on the type species,
R. maculata
CCMP 736. We have emended the previously established orders Dixoniellales and Rhodellales to cor-rect the placement of the genera
Corynoplastis, Dixoni-ella, Neorhodella
, and
Rhodella
and established the new order
Glaucosphaerales
to accommodate a single genus,
Glaucosphaera
.
MATERIALS AND METHODS
- Culture methods
Procedures for isolation and culture were as described in West (2005). Isolate JAW 2347 (CCMP 3129) was ob-tained from a mud sample in a
Salicornia
salt marsh at Bodega Bay, CA, USA on April 15, 1980. O’Kelly’s isolates RV-FHLa & b (CCMP 3133, 3135) were obtained as epi-phytes on the green alga
Capsosiphon
sp. on a seawall at Friday Harbor, WA, USA in March 2010. These three iso-lates are available from Bigelow Laboratory for Ocean Sci-ences, P. O. Box 475, 180 McKown Point Road, West Booth-bay Harbor, ME 04575, USA.
- Confocal and Nomarski microscopy
Fixation, staining, and observation methods were par-tially described in Yang et al. (2011). Cultured cells were transferred to 1.5 mL microtubes with 0.5 mL of culture medium, fixed with an equal volume of 3% paraformal-dehyde in PHEM buffer [60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 2 mM MgCl
2
(6H
2
O), pH 7.4] for 15 min. Ten μL of fixed cells were placed on a glass slide coated with 0.1 w/v% poly-L-lysine and incubated for 10 min at room temperature. Then equal volumes of SlowFade Antifade (Invitrogen, Carlsbad, CA, USA) and 1,000× diluted SYBR Green I (Invitrogen) were added and incubated for 15 min. Confocal images were taken with an inverted microscope Axiovert 100M with LSM 510 laser scanning equipment (Carl Zeiss AG, Jena, Germany) at the University of Tsuku-ba, and an Axio Observer LSM 700 instrument at Bigelow Laboratory for Ocean Sciences. Plastid autofluorescence and SYBR Green I fluorescence were detected with a 585 nm long pass filter and a 505 to 530 nm band pass filter, respectively, in the excitation line of a 488 nm argon laser and 543 nm He / Ne laser using a single-track mode. Liv-ing cells were observed with a Nikon microscope (Eclipse E600) equipped with Nomarski interference optics (Nikon Co. Ltd., Tokyo, Japan).
- Transmission electron microscopy
Cells from cultures (grown in window light in 15 psu von Stosch enriched seawater) were filtered onto poly-L-lysine coated 0.45 μm Millipore filters (Bedford, MA, USA) and fixed for 2 h at ambient temperature in 2% glutaraldehyde in a 0.1 M phosphate buffer solution (pH 6.8) with 0.15 M sucrose. Following buffer rinses samples were post-fixed 2 h in the same buffer in 1% OsO
4
at 4℃, rinsed thoroughly in H
2
O, left in 50% acetone for 30 min, and stored in a 70% acetone-2% uranyl acetate solution at ambient temperature for 22 h. Samples were then further dehydrated in a graded acetone series, infiltrated gradu-ally, embedded in Embed 812 resin (Electron Microscopy Sciences, Hatfield, PA, USA), and polymerized at 70℃ for 2-3 days. Thin sections were cut with an RMC MT6000-XL ultramicrotome (RMC Inc., Tucson, AZ, USA). Sections were stained for 1 min with lead acetate. A Zeiss EM 109 electron microscope was used for observation and pho-tography.
- DNA extraction, amplification and sequencing
Genomic DNA was extracted from each culture strain using a DNeasy Plant Mini Kit (Qiagen, Hilden, Ger-many) according to the manufacturers’ instructions. Polymerase chain reaction (PCR) and sequencing were performed with published and newly designed spe-cific primer sets for each gene; psaA130F-psaA1110R, psaA870F-psaA1600R and psaA971F-psaA1760R for
psa
A (Yoon et al. 2002, Yang and Boo 2004),
rbc
L43F (5'-CGT-TAYGAATCTGGTGTAATYCC-3')-rbcL1414R (5'-TCAGCTG-TATCTGTAGAAGTATA-3') for
rbc
L. PCR amplification was performed in a total volume of 25 μL, containing 0.02 unit of Phusion High-Fidelity DNA polymerase (Finnzymes OY, Espoo, Finland), 5 μL of the 5× Phusion HF Buffer (contain 1.5 mM MgCl
2
), 200 μM of each dNTPs, 10 μM of each primer and 1-20 ng of template DNA. PCR was carried out with an initial denaturation at 98℃ for 30 s, followed by 30 main amplification cycles of denaturation at 94℃ for 10 s, annealing at 50-55℃ for 30 s, extension krat 72℃ for 1 min, and a final extension at 72℃ for 7 min. Amplified DNA was purified with the QIAquick PCR Puri-fication kit (Qiagen) and sent to a commercial sequencing company. Electropherogram outputs for each specimen were edited using the program Chromas v.1.45 (http://www.technelysium.com.au/chromas.html). Newly de-termined sequences were deposited in the GenBank da-tabases (http://www.ncbi.nlm.nih.gov) under the acces-sion numbers JN934872-JN934889 (see detail in
Table 1
).
- Phylogenetic analyses
Published sequences were obtained from GenBank and aligned with newly determined sequences using ClustalW implemented SeaView version 4.2.5 (Gouy et al. 2010) and manually refined using Se-Al version 2.0a11 (http://tree.bio.ed.ac.uk/software/seal/). We excluded insertions of the nuclear small subunit rRNA (SSU rRNA) (
Rhodella
sp. MBIC10593, AB183619: #538-1045;
Thorea violacea
SAG 51.94, AF342744: #1356-1479) and introns of plastid
psa
A (
Rhodella maculata
CCMP 736, DQ308448: #79-858;
Rhosospora sordida
UTEX 2621, DQ308453: #226-723). There were no indel in
psb
A and
rbc
L. We used two data sets for tree search, (i) DNA and protein mixed data (total 3,070 characters; 1,881 base pairs of nuclear SSU rRNA + 1,189 amino acid sequences from plastid
psa
A,
psb
A, and
rbc
L) and (ii) DNA data (total 5,448 bp; 1,881 bp SSU rRNA + 3,567 bp plastid genes).
The evolutionary model for individual genes was cho-sen using the weighted Akaike information criterion (AIC) implemented in ModelGenerator version 0.85 (Keane et al. 2006). The selected best fitting models were the gen-eral time reversible (GTR) substitution with the gamma distributed rate heterogeneity (G) for DNA data; the LG substitution (Le and Gascuel 2008) with empirical amino acid frequencies (F) and G (LG + F + G model) for protein data. We used an independent model for each partition of the concatenated data. For example, it was used GTR + G model for SSU part and separate LG + F + G for
psa
A,
psb
A and
rbc
L parts of mixed data; separate GTR + G for individual gene of DNA data.
Maximum likelihood (ML) analyses were performed using RAxML version 7.2.8 (Stamatakis 2006). Tree like-lihoods were estimated using 200 independent replica-tions, each with a random starting point. The separate site-specific model was used for partitioned data and the automatically optimized SPR branch rearrangements were used during the rapid hill climbing tree search for each replication. Bootstrap analyses (MLB) were con-ducted using 1,000 replications with the same evolu-
Taxa list used in present studyGenBank accessions for new sequences are in bold.
Taxa list used in present study GenBank accessions for new sequences are in bold.
tionary model setting as that used for the best topology search.
RESULTS
- Confocal observations
The following observations were made with the culture isolate JAW 2347 (CCMP 3129). SYBR Green stain gave a green fluorescence to nuclear DNA as well as chloro-plast and mitochondria genophores, whereas the whole chloroplast showed a red autofluorescence (
Fig. 1
). The nucleus of the interphase stage was always eccentric and associated with a pyrenoid (
Fig. 1
A-C). The single chlo-roplast had its pyrenoid centrally located and had many lobes positioned at the cell periphery. Plastid genophores were spherical to subspherical (about 0.25 μm diameter) and present in the peripheral lobes. Two nuclei were found in some dividing cells (
Fig. 1
D). All observed char-acters were also found in
R. maculata
CCMP 736 (
Fig. 1
E & F), without any features distinguishing it from JAW 2347 (CCMP 3129).
- Transmission electron microscopy
The cells were 8-12 μm in diameter and were coated by thin fibrillar material (
Fig. 2
). A discontinuous layer of ER was present beneath the cell membrane and appeared interconnected with the cell membrane by short tubules (
Fig. 2
&
3
D), as seen by Patrone et al. (1991) in
Rhodella violacea, R. maculata
and many other unicellular red al-gae. The spherical nucleus was approximately 3 μm in di-ameter, occupying a peripheral location and was always closely associated with the more central pyrenoid (
Fig. 2
). The nucleus contained one mostly central nucleolus (
Fig. 2
). In favorable sections usually one but occasionally two nuclear protrusions could be seen penetrating the pyre-noid as deep as 0.5-1 μm (
Fig. 2
&
3
A). The protrusions always contained an electron dense, fibrillar material, not well seen in
Fig. 2
&
3
A. In the examination of several hundred cells, nuclear protrusions were detected in over 20.
Usually one pyrenoid was found in each cell but a few cells contained two pyrenoids. The pyrenoids were com-monly larger than nuclei and were often bordered by starch that was also seen elsewhere in the cell, frequently near nuclei (
Fig. 2
). The moderately electron dense py-renoid matrix was devoid of thylakoids (
Fig. 2
&
3
A). As observed in two-dimensional sections, 1-4 chloroplast lobes extended from the pyrenoid throughout the cy-toplasm. Thylakoids were abundant and maintained consistent spacing from each other (
Fig. 2
,
3
B & C), but phycobilisomes were not usually clearly seen. Peripheral thylakoids were absent (
Fig. 2
&
3
C). Electron dense clus-ters of spherical globules (plastoglobuli) were commonly seen at the outermost periphery of chloroplast lobes (
Fig. 3
C). Profiles of mitochondria were found throughout the cell (
Fig. 2
,
3
B & C) as well as small electron transpar-ent vacuoles (
Fig. 2
&
3
B). Golgi bodies were somewhat sparse and usually located at peripheral cell regions. All observed Golgi bodies had fused or closely appressed cis-ternae (
Fig. 3
B).
- Four-gene phylogeny
Newly determined rhodellophycean nuclear SSU rRNA and three plastid protein-coding
psa
A,
psb
A, and
rbc
L genes were aligned and used for the phylogenetic anal-ysis. ML trees based on DNA + protein mixed data and
Morphological characters from Rhodella violacea (JAW 2347 / CCMP 3129, A-D) and R. maculata (CCMP 736, E-F). (A) Nomarski differential interference microscopic image shows a single pyrenoid filled with uniform material and surrounded by starch. Plastid globules (yellowish orange) are scattered at the cell surfaces. A single eccentric nucleus is situated beside the pyrenoid. (B) Confocal microscopic image from single focal level. Fluorescent SYBR Green staining show a single nucleus with single nucleolus and chloroplast genophores as small particles in the lobes at the cell perimeter. (C) Combined image of SYBR Green staining and plastid autofluorescence (red). A pyrenoid region is the central dark space. Plastid geno-phores (greenish-yellow) are located in the central areas of chloroplast lobes. (D) Dividing cell with two nuclei. (E) Confocal microscopy of CCMP 736 shows a single nucleus and chloroplast genophores similar to JAW 2347. (F) Combined images of SYBR Green staining and plastid autofluorescence from CCMP 736. Scale bars represent: A-F, 2.0 μm.
Rhodella violacea (JAW 2347 / CCMP 3129) ultrastructure. Low magnification electron micrograph of a medially sectioned cell. The nucleus (N) is peripheral with a centrally located nucleolus (NL). A nuclear extension (arrow) projects into the adjacent pyrenoid (P), which is sur-rounded by starch grains (S). The pyrenoid lacks thylakoids and is seen connected to one of the chloroplast lobes (asterisk). A peripheral thylakoid is absent, as detected in several regions of the chloroplast lobes (arrowheads). A peripheral endoplasmic reticulum (ER) system (PER) is visible in some areas just in side of the cell membrane. Scale bar represents: 1.0 μm.
Rhodella violacea (JAW 2347 / CCMP 3129) ultrastructure. (A) The nuclear protrusion contains electron dense material. N, nucleus; P, py-renoid; S, starch. (B) A Golgi body (G) is near the cell membrane (arrow). An expanded portion of endoplasmic reticulum (ER) is seen between the cell membrane and the Golgi cis-region. Golgi cisternae are closely appressed in the mid-region. C, chloroplast lobe. (C) Electron dense globules (plastoglobuli, arrows) are consistently located at the periphery of chloroplast lobes. M mitochondrion. (D) Tubules of the peripheral ER system (arrows) are seen in cross section. Scale bars represent: A-C, 0.5 μm; D, 0.1 μm.
DNA data were consistent with each other, except for the position of Porphyridiophyceae (
Fig. 4
). The Porphyrid-iophyceae grouped with a Bangiophyceae / Florideophy-ceae / Rhodellophyceae clade in the DNA + protein mixed data, whereas it grouped together with the Compsopogo-nophyceae / Stylonematophyceae clade in the DNA-only tree. However, this incongruence was not supported sta-tistically (less than 50% bootstrap value in each phylog-eny).
Multigene trees supported the monophyly of Rhodel-lophyceae strongly (MLB and mixed and DNA 100%). Within the rhodellophycean monophyly, two subclades were identified: i) the Rhodellales clade (MLB mixed and DNA 100%) including
Rhodella
and
Corynoplastis
, ii) the Glaucosphaerales and Dixoniellales clade (MLB mixed 97% and DNA 100%), including
Glaucosphaera, Dixoni-ella
, and
Neorhodella
. Within Rhodellales, all 16
Rhodella
isolates were strongly grouped together (MLB mixed and DNA 100%). However, they did not make monophyletic groups for each species of
R. violacea
(BRW, UTEX 2427, SAG 115.79) and
R. maculata
(CCMP 736, CCAP 1388/6, Japan: Amami).
Rhodella
sp. BC73C was separated from the rest of the isolates (MLB mixed 91% and DNA 94%). Four isolates (MBIC11021, MBIC10825, MBIC10593 and Japan: Amani) were grouped together (MLB mixed 79% and DNA 77%).
Sequence divergences within
Rhodella
isolates ranged from 0 to 37 bp (2% p distance; between MBIC 10593 and GA6-T4) in SSU rRNA, up to 18 bp (1.5%; between JAW 2347 and UTEX 2427) in
rbc
L, up to 6 bp (0.7%; between RV-FHLa and SAG 115.79) in
psb
A, and up to 2 bp (0.1%; between CCMP 736 and SAG 115.79) in
psa
A. Sequence divergences between
Rhodella
sp. BC73C and the rest of the isolates were clearly higher, ranging from 68 (3.6%, with
R. violacea
UTEX 2427) to 78 bp (4.1%, with
R. macu-lata
Japan: Amani isolate) in the SSU rRNA sequence.
DISCUSSION
Our ultrastructural examination of JAW 2347 revealed two cell characters found in no other unicellular red al-gae except
R. maculata
and
R. violacea
(Evans 1970, Weh-rmeyer 1971, Patrone et al. 1991): the pyrenoid matrix lacked thylakoids and was penetrated by one or more pro-trusions from the closely associated nucleus. Also present in
Rhodella
were Golgi bodies associated only with ER, a chloroplast lacking a peripheral encircling thylakoid, and accumulations of electron dense globules (plastoglobuli) in the outer chloroplast lobes at the cell periphery.
The only other member of the Rhodellophyceae pos-sessing Golgi bodies associated with ER is
Corynoplastis
. Golgi bodies in
Dixoniella, Glaucosphaera
, and
Neorho-della
are consistently situated near the outer membrane of the nuclear envelope. No other red alga has this pecu-liar Golgi association. However, the fact that the ER and the outer membrane of the nuclear envelope are both functional equivalents is what unifies Golgi differences in the Rhodellophyceae (see discussion in Scott et al. 2008). In contrast, Golgi bodies in all members of the Porphy-ridiophyceae, Bangiophyceae, and Florideophyceae are always closely associated with mitochondria. Only the relatively few genera in the Stylonematophyceae and Compsopogonophyceae have an ER-Golgi alignment as is routinely found in most other eukaryotes. An additional Golgi attribute found only in the other rhodellophycean unicells and sporangia of certain multicellular red algae (Scott et al. 2008) is fusion or apposition of Golgi cister-nae. The significance of this unusual trait is unknown.
The presence or absence of a peripheral thylakoid is variable in the Rhodellophyceae.
Dixoniella, Glauco-sphaera
, and
Corynoplastis
possess a peripheral thy-lakoid, whereas
Rhodella
and
Neorhodella
do not. The four genera of Porphyridiophyceae,
Erythrolobus, Flinti-ella, Porphyridium
, and
Timspurckia
all lack this fea-ture. Within the Stylonematophyceae, Compsopogono-phyceae, Bangiophyceae, and Florideophyceae only the sporophyte (conchocelis) stages of
Bangia
and
Porphyra
(Pueschel 1990) and vegetative cells of
Rhodachlya
(Rho-dachlyaleales, Florideophyceae) (West et al. 2008) lack a peripheral thylakoid. The phylogenetic and functional significance of the presence or absence of this thylakoid is unknown. Electron dense globules or plastoglobuli are fairly commonly seen in red algal chloroplasts (Pueschel 1990). Plastoglobuli clusters located in the peripheral lobes of chloroplasts are present in all members of Rho-dellophyceae but are absent in other unicellular red algae (Scott et al. 2008, Yokoyama et al. 2009). See Deason et al. (1983) for a detailed discussion of plastoglobuli (stigmata = eyespots) in chloroplasts of unicellular red algae.
It is obvious that the presence or absence of starch grains closely bordering the naked pyrenoids of
Rhodella
is a variable. Some cells of JAW 2347 isolate (CCMP 3129) had pyrenoids free of starch, whereas other cells showed a close association. Pyrenoids in
R. violacea
cells observed by Wehrmeyer (1971) were surrounded by starch, where-as cells of the same culture strain studied by Patrone et al. (1991) lacked starch in the region adjacent to the nucleus where the nuclear protrusions are located. However, as Wehrmeyer (1971) did not find any nuclear protrusions
The maximum likelihood phylogeny of Rhodella violacea is based on nuclear small subunit (SSU) rRNA + plastid protein psaA, psbA, and rbcL (-lnL = 26232.95). Likelihood is estimated under the general time reversible (GTR) + G model for the DNA part and separate LG + G + F model for the individual protein gene part. The maximum likelihood (ML) bootstrap support values are shown on the branches (from DNA + protein mixed data) and under branch (from DNA data).
in his study, it is possible that starch could likewise be ab-sent in the protrusion zone of the isolate of
R. violacea
that he examined. All regions of pyrenoids in
R. maculata
are bordered by starch grains, even between the pyrenoids and nuclei in the protrusion zone (Evans 1970, Patrone et al. 1991). However, in
R. maculata
cells studied by Waller and McFadden (1995), several micrographs showed py-renoids heavily bordered by starch, whereas one micro-graph showed a starch-free pyrenoid. Most likely these observed variations in starch content and localization are more likely dependent upon differences in culture condi-tions used or cell cycle stage studied rather then reliable indicators to discriminate taxa.
The nuclear protrusions in strain JAW 2347 (CCMP 3129),
R. violacea
(Patrone et al. 1991), and those in
R. maculata
observed by Evans (1970), Patrone et al. (1991), and Waller and McFadden (1995) were variable length but all contained an electron dense material. Waller and McFadden (1995) determined that the material was non-ribosomal RNA and could possibly serve a structural role. Two protrusions were occasionally seen in a single cell of
R. violacea
(Patrone et al. 1991) and, two protrusions were commonly found by Waller and McFadden (1995) in
R. maculata
cells by serial sectioning.
The low molecular weight carbohydrate (LMWC) man-nitol is present in all Rhodellophycae including JAW 2347 (CCMP 3129) (Karsten et al. 1999, 2003, Scott et al. 2008, Nitschke et al. 2010).
JAW 2347 (CCMP 3129) is readily recognized as a species of
Rhodella
based on electron microscopic and molecular evidence. As seen in thin sections: (1) Golgi bodies were consistently associated with ER and no other organelles (e.g., mitochondria or the nucleus), (2) the chloroplast is highly dissected with thylakoid-filled lobes containing peripheral plastoglobuli and no peripheral thylakoid, (3) the matrix of the central to eccentric pyrenoid is devoid of thylakoids but penetrated by one or two unique digitate protrusions arising from the adjacent nucleus. No other unicellular red algal genus has this combination of ultra-structural characters (Scott et al. 2008).
The concatenated phylogeny (nuclear SSU rRNA + plastid
psa
A,
psb
A, and
rbc
L) is congruent with the sev-en-class system of Rhodophyta and the monophyly of Rhodellophyceae (Yoon et al. 2006, 2010). In the present study, as rhodellophycean ingroup taxa increased, there was greater ML bootstrap support for the sister relation-ship of Rhodellophyceae to the Bangio-Florideophyceae clade than that of previous studies, i.e., >50% from SSU rRNA tree in Yokoyama et al. (2009); 53% from a nine-gene tree in Yoon et al. (2006); 58% from a two-gene tree in Yang et al. (2010). These results support the idea that broad taxa sampling increased robustness of the tree (Ro-kas and Carroll 2005, Parfrey et al. 2010). Our phylogeny suggest that the Rhodellophyceae is the sister class of the Bangiophyceae-Florideophyceae clade. Within the Rho-dellophyceae, the Glaucosphaerales and Dixoniellales are sister group taxa separated from the Rhodellales.
In the
Rhodella
phylogeny, all
Rhodella
isolates (ex-cept isolate BC73C) were grouped together, and neither
R. violacea
nor
R. maculata
were a monophyletic group in the genus. Most isolates were strongly grouped together in the tree. The results showed low sequence divergences in SSU rRNA (2%) and three plastid genes (< 2%), which were significantly lower than those of other unicellular red algae (e.g., 11-15% divergence in
psa
A of
Erythrolobus
spp.) (Yang et al. 2010). However, isolate BC73C is geneti-cally distinct within
Rhodella
and may be a distinct spe-cies, but further investigation is required.
- Redefinition of the Rhodellophyceae orders and families
Wynne and Schneider (2010) indicated that the family Dixoniellaceae established by Yokoyama et al. (2009) was invalid because the family Glaucosphaeraceae, which originally included only
Glaucosphaera
, was established by Skuja (1954) and had precedence. However, based on the structural characters, cell activity (i.e., continuous Golgi vesicle formation and extrusion), and molecular evidence, we have placed the genus
Glaucosphaera
in the separate new order Glaucosphaerales Yang, Scott, Yoon and West in which the family Glaucosphaeraceae Skuja is now placed. The Glaucosphaerales is phylogenetically closely related to the Dixoniellales.
Glaucosphaerales ord. nov., Yang, Scott, Yoon and West
. The order, family and genus are defined as a unicel-lular freshwater red alga, containing a blue green chloro-plast with a peripheral thylakoid, plastoglobuli clusters, no pyrenoid, active perinuclear Golgi bodies continuous-ly producing and rapidly ejecting small vesicles from the cell, cells larger than most unicellular reds (to 25 μm di-ameter) (Broadwater et al. 1995, Pickett-Heaps et al. 2001, Wilson et al. 2006), LMWCs unknown.
Glaucosphaeraceae Skuja (1954)
. Same characters as the order. The only taxon at present is
Glaucosphaera vacuolata
Korshikov (1930), known as the original type specimen from a large pond in a meadow at the outskirts of the city Charkova (Kharkov), Ukraine, in August 1929 and a single collection and culture obtained by Richard Starr in May 1968 in soil from a horse pond near Elletts-ville, IN, USA.
Dixoniellales and Dixoniellaceae Yokoyama et al. (2009)
. Emended ordinal and family description: single chloroplast with a single or multiple pyrenoids, plasto-globuli at chloroplast periphery, Golgi bodies perinuclear, mannitol as the only LMWC.
Dixoniella
and
Neorhodella
are now the only genera in this order and family.
Rhodellales Yoon et al. (2006)
. As originally defined by Yoon et al. (2006) this order includes
Rhodella, Dixoniella
and
Glaucosphaera
. Yokoyama et al. (2009) placed
Dix-oniella
and
Neorhodella
in the Dixoniellales Yokoyama et al. (2009).
Emended ordinal description: unicellular red algae, a single highly lobed plastid with single or multiple pyre-noids, plastoglobuli at plastid periphery, scattered Golgi associated with ER, contain mannitol as a LMWC.
Rhodellaceae Yoon et al. (2006) now contains only two genera
Rhodella
and
Corynoplastis
(Yokoyama et al. 2009). The genus description of
Rhodella
is emended to specify that digitate nuclear intrusions occur in the pyre-noid and the pyrenoid matrix is devoid of thylakoids. The two currently recognized species [
R. maculata
Evans and
R. violacea
(Kornmann) Wehrmeyer] are merged as one,
R. violaceum
(Kornmann) Wehrmeyer.
Rhodella violacea
is used because it is the basionym
Porphyridium viola-ceum
Kornmann (1965), and the name with priority.
Acknowledgements
Laboratory culture investigations were partially sup-ported by grants to JAW from the Australian Research Council, Australian Biological Resources Study and Her-mon Slade Foundation. This project was also partially supported by the National Science Foundation (DEB-0937975, 0934176), USA and the Marine Biotechnology Program funded by Ministry of Land, Transport, and Mar-itime Affairs, Korea to HSY, and by the Japan Society for the Promotion of Science (Grants-in-Aid for Scientific Re-search 20570081) to AY. We appreciate the valuable com-ments from the reviewer that improved the manuscript immensely.
View Fulltext
Billard C
,
Fresnel J
1986
Rhodella cyanea nov. sp. une nouvelle Rhodophyceae unicellulaire.
C. R. Acad. Sci. Serie IIII Sci. Vie
302
271 -
276
Broadwater S. T
,
Scott J. L
,
Goss S. P. A
,
Saunders B. D
1995
Ultrastructure of vegetative organization and cell division in Glaucosphaera vacuolata Korshikov (Porphy-ridiales Rhodophyta).
Phycologia
34
351 -
361
DOI : 10.2216/i0031-8884-34-5-351.1
Evans L. V
1970
Electron microscopical observations on a new red algal unicell Rhodella maculata gen. nov. sp. nov.
Br. Phycol. J.
5
1 -
13
DOI : 10.1080/00071617000650011
Fresnel J
,
Billard C
,
Hindák F
,
Pekárková B
1989
New observations on Porphyridium griseum Geitler = Rho-della grisea (Geitler) comb. nova (Porphyridiales Rho-dophyceae).
Plant Syst. Evol.
164
253 -
262
DOI : 10.1007/BF00940441
Giraud G
1962
Les infrastructures de quelques algues et leur physiologie.
J. Microsc.
1
251 -
274
Gouy M
,
Guindon S
,
Gascuel O
2010
SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building.
Mol. Biol. Evol.
27
221 -
224
DOI : 10.1093/molbev/msp259
Karsten U
,
West J. A
,
Zuccarello G. C
,
Engbrodt R
,
Yo-koyama A
,
Hara Y
,
Brodie J
2003
Low molecular weight carbohydrates of the Bangiophycidae (Rho-dophyta).
J. Phycol.
39
584 -
589
DOI : 10.1046/j.1529-8817.2003.02192.x
Karsten U
,
West J. A
,
Zuccarello G. C
,
Nixdorf O
,
Barrow K. D
,
King R. J
1999
Low molecular weight carbohy-drate patterns in the Bangiophyceae (Rhodophyta).
J. Phycol.
35
967 -
976
DOI : 10.1046/j.1529-8817.1999.3550967.x
Keane T. M
,
Creevey C. J
,
Pentony M. M
,
Naughton T. J
,
McInerney J. O
2006
Assessment of methods for amino acid matrix selection and their use on empirical data shows that ad hoc assumptions for choice of matrix are not justified.
BMC Evol. Biol.
6
29 -
DOI : 10.1186/1471-2148-6-29
Kornmann P
1965
Porphyridium violaceum eine marine neue
Art. Helgol. Wiss. Meeresunters.
12
420 -
423
DOI : 10.1007/BF01612563
Korshikov A. A
1930
Glaucosphaera vacuolata a new mem-ber of the Glaucophyceae.
Arch. Protistenkunde
70
217 -
222
Le S. Q
,
Gascuel O
2008
An improved general amino acid replacement matrix.
Mol. Biol. Evol.
25
1307 -
1320
DOI : 10.1093/molbev/msn067
Nitschke U
,
Boedeker C
,
Karsten U
,
Hepperle D
,
Eg-gert A
2010
Does the lack of mannitol accumulation in an isolate of Rhodella maculata (Rhodellophyceae Rhodophyta) from the brackish Baltic Sea indicate a stressed population at the distribution limit?
Eur. J. Phy-col.
45
436 -
449
DOI : 10.1080/09670262.2010.501908
Parfrey L. W
,
Grant J
,
Tekle Y. I
,
Lasek-Nesselquist E
,
Mor-rison H. G
,
Sogin M. L
,
Patterson D. J
,
Katz L. A
2010
Broadly sampled multigene analyses yield a well-resolved eukaryotic tree of life.
Syst. Biol.
59
518 -
533
DOI : 10.1093/sysbio/syq037
Patrone L. M
,
Broadwater S. T
,
Scott J. L
1991
Ultra-structure of vegetative and dividing cells of the unicellu-lar red algae Rhodella violacea and Rhodella maculata.
J. Phycol.
27
742 -
753
DOI : 10.1111/j.0022-3646.1991.00742.x
Pickett-Heaps J. D
,
West J. A
,
Wilson S. M
,
McBride D. L
2001
Time-lapse video microscopy of cell (spore) move-ment in red algae.
Eur. J. Phycol.
36
9 -
22
DOI : 10.1080/09670260110001735148
Pueschel C
1990
Cell structure. In Cole K. M. & Sheath R. G. (Eds.) Biology of the Red Algae.
Cambridge University Press
New York
7 -
41
Rokas A
,
Carroll S. B
2005
More genes or more taxa? The relative contribution of gene number and taxon number to phylogenetic accuracy.
Mol. Biol. Evol.
22
1337 -
1344
DOI : 10.1093/molbev/msi121
Scott J
,
Yokoyama A
,
Billard C
,
Fresnel J
,
Hara Y
,
West K. A
,
West J. A
2008
Neorhodella cyanea a new ge-nus in the Rhodellophyceae (Rhodophyta).
Phycologia
47
560 -
572
DOI : 10.2216/08-27.1
Scott J. L
,
Broadwater S. T
,
Saunders B. D
,
Thomas J. P
,
Gabrielson P. W
1992
Ultrastructure of vegetative or-ganization and cell division in the unicellular red alga Dixoniella grisea gen. nov. (Rhodophyta) and a consid-eration of the genus Rhodella.
J. Phycol.
28
649 -
660
DOI : 10.1111/j.0022-3646.1992.00649.x
Skuja H
1954
Abteilung: Glaucophyta. In Melchior H. & Werdermann E. (Eds.) A. Englers Syllabus der Pflanzen-familien 12. Aufl. 1. Band.
Borntraeger
Berlin
56 -
57
Stamatakis A
2006
RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models.
Bioinformatics
22
2688 -
2690
DOI : 10.1093/bioinformatics/btl446
Waller R. F
,
McFadden G. I
1995
Morphological and cy-tochemical analysis of an unusual nucleus-pyrenoid as-sociation in a unicellular red alga.
Protoplasma
186
131 -
141
DOI : 10.1007/BF01281323
Wehrmeyer W
1971
Elektronmikroskopische Untersuc-hung zur Feinstruktur von Porphyridium violaceum Kornmann mit Bemerkungen über seine taxonomische Stellung.
Arch. Mikrobiol.
75
121 -
139
DOI : 10.1007/BF00408000
West J. A
2005
Long term macroalgal culture maintenance. In Anderson R. (Ed.) Algal Culturing Techniques.
Aca-demic Press
New York
157 -
163
West J. A
,
Scott J. L
,
West K. A
,
Karsten U
,
Clayden S. L
,
Saunders G. W
2008
Rhodachlya madagascarensis gen. et sp. nov.: a distinct acrochaetioid represents a new order and family (Rhodachlyales ord. nov. Rhodachly-aceae fam. nov.) of the Florideophyceae (Rhodophyta).
Phycologia
47
203 -
212
Wilson S. M
,
Pickett-Heaps J. D
,
West J. A
2006
Vesicle transport and the cytoskeleton in the unicellular red alga Glaucosphaera vacuolata.
Phycol. Res.
54
15 -
20
DOI : 10.1111/j.1440-1835.2006.00404.x
Wynne M. J
,
Schneider C. W
2010
Addendum to the syn-optic review of red algal genera.
Bot. Mar.
53
291 -
299
DOI : 10.1515/bot.2010.039
Yang E. C
,
Boo S. M
2004
Evidence for two independent lineages of Griffithsia (Ceramiaceae Rhodophyta) based on plastid protein-coding psaA psbA and rbcL gene se-quences.
Mol. Phylogenet. Evol.
31
680 -
688
DOI : 10.1016/j.ympev.2003.08.014
Yang E. C
,
Scott J
,
West J. A
,
Orlova E
,
Gauthier D
,
Küp-per F. C
,
Yoon H. S
,
Karsten U
2010
New taxa of the Porphyridiophyceae (Rhodophyta): Timspurckia oligo-pyrenoides gen. et sp. nov. and Erythrolobus madagasca-rensis sp. nov.
Phycologia
49
604 -
616
DOI : 10.2216/09-105.1
Yang E. C
,
Scott J
,
West J. A
,
Yoon H. S
,
Yokoyama A
,
Karsten U
,
Loiseaux de Goër S
,
Orlova E
2011
Erythrolobus australicus sp. nov. (Porphyridiophyceae Rhodophyta): a description based on several approach-es.
Algae
26
167 -
180
DOI : 10.4490/algae.2011.26.2.167
Yokoyama A
,
Sato K
,
Hara Y
2004
The generic delimi-tation of Rhodella (Porphyridiales Rhodophyta) with emphasis on ultrastructure and molecular phylogeny.
Hydrobiologia
512
177 -
183
DOI : 10.1023/B:HYDR.0000020325.08825.14
Yokoyama A
,
Scott J. L
,
Zuccarello G. C
,
Kajikawa M
,
Hara Y
,
West J. A
2009
Corynoplastis japonica gen. et sp. nov. and Dixoniellales ord. nov. (Rhodellophyceae Rhodophyta) based on morphological and molecular evidence.
Phycol. Res.
57
278 -
289
DOI : 10.1111/j.1440-1835.2009.00547.x
Yoon H. S
,
Hackett J. D
,
Bhattacharya D
2002
A single origin of the peridinin- and fucoxanthin-containing plastids in dinoflagellates through tertiary endosymbio-sis.
Proc. Natl. Acad. Sci. U. S. A.
99
11724 -
11729
DOI : 10.1073/pnas.172234799
Yoon H. S
,
Müller K. M
,
Sheath R. G
,
Ott F. D
,
Bhat-tacharya D
2006
Defining the major lineages of red al-gae (Rhodophyta).
J. Phycol.
42
482 -
492
DOI : 10.1111/j.1529-8817.2006.00210.x
Yoon H. S
,
Zuccarello G. C
,
Bhattacharya D
2010
Evolu-tionary history and taxonomy of red algae. In Seckbach J. & Chapman D. J. (Eds.) Red Algae in the Genomic Age: Cellular Origin Life in Extreme Habitats and Astrobiol-ogy.
Springer
Heidelberg
25 -
42