EXPERIMENTAL SECTION
- Instruments
1
H- and
13
C-NMR spectra were taken on a Bruker DMX 600 NMR spectrometer using TMS as an internal standard. FAB mass spectra were recorded on a Krapos mass spectrometer using
m
-nitrobenzyl alcolhol as a matrix.
- Fermentation and Isolation
The first seed-culture of the organism, prepared by incubation at 30
o
C for 2 days on a reciprocal shaker (180 rpm) in Tryptic Soy Broth (TBS, Sigma, 50 mL) was inoculated into the same medium and cultured under the same culture condition for the second seed-culture. The production culture medium (20 liters) was chosen among the three media (
4
), which was cultured on a jar fermentor for 4 days at 30
o
C with an air flow of 2−3 liters per minute and agitation rate of 175 rpm. The harvested cells were extracted with MeOH, and the extract was chromatographed on a column packed with silica gel which was eluted with CH
2
Cl
2
−MeOH (30:1, v/v) to give a mixture of four macrolactone class of compounds
1−4
. Each of the components was finally purified by reverse-phase HPLC (Inertsil ODS-2, 5 μm, i.d. 4.6×150 mm: solvent system: CH
3
CN−H
2
O = 1:1 (v/v): detection: UV at 275 nm).
Mulligan M. E.
,
Murray-Leisure K. A.
,
Ribner B. S.
,
Standford H. C.
,
John J. F.
,
Korvick J. A.
,
Kauffman C. A.
,
Yu V. L.
1993
Am. J. Med.
94
313 -
DOI : 10.1016/0002-9343(93)90063-U
Crum N. F.
,
Lee R. U.
,
Thornton S. A.
,
Stine O. C.
,
Wallace M. R.
,
Barrozo C.
,
Keefer-Norris A.
,
Judd S.
,
Russell K. L.
2006
Am. J. Med.
119
943 -
DOI : 10.1016/j.amjmed.2006.01.004
Graninger W.
,
Weinisch C.
,
Hasenhundl M.
1995
Curr. Opin. Infect. Dis.
8
(Suppl. 1)
520 -
Low D. E.
,
Nadler H. L.
1997
J. Antimicrob. Chemother.
39
(Suppl. A)
53 -
Hiramatus K.
,
Hanaki H.
,
Ino T.
,
Yabuta K.
,
Oguri, T. Tenover F. C.
1997
J. Antimicrob. Chemother.
40
135 -
DOI : 10.1093/jac/40.1.135
Berdy J.
1980
CRC Handbook of Antibiotic Compounds
CRC Press
Boca Raton, Florida, U.S.A.
Omura S.
1984
In Macrolide Antibiotics: Chemistry, Biology, and Practice; Omura, S.; Tanaka, H, Ed.
Academic Press
London, U. K.