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New Macrolactone Secondary Metabolites Produced from a Strain of Cytophaga sp.
New Macrolactone Secondary Metabolites Produced from a Strain of Cytophaga sp.
Journal of the Korean Chemical Society. 2013. Jun, 57(3): 416-419
Copyright © 2013, Korea Chemical Society
  • Received : March 11, 2013
  • Accepted : April 04, 2013
  • Published : June 20, 2013
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About the Authors
Nam-Gin Jung
Beom-Tae Kim
Department of Bioactive Material Sciences, College of Natural Science, Research Center of Bioactive Materials, Chonbuk National University, Chonju 561-756, Korea.

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EXPERIMENTAL SECTION
- Instruments
1 H- and 13 C-NMR spectra were taken on a Bruker DMX 600 NMR spectrometer using TMS as an internal standard. FAB mass spectra were recorded on a Krapos mass spectrometer using m -nitrobenzyl alcolhol as a matrix.
- Fermentation and Isolation
The first seed-culture of the organism, prepared by incubation at 30 o C for 2 days on a reciprocal shaker (180 rpm) in Tryptic Soy Broth (TBS, Sigma, 50 mL) was inoculated into the same medium and cultured under the same culture condition for the second seed-culture. The production culture medium (20 liters) was chosen among the three media ( 4 ), which was cultured on a jar fermentor for 4 days at 30 o C with an air flow of 2−3 liters per minute and agitation rate of 175 rpm. The harvested cells were extracted with MeOH, and the extract was chromatographed on a column packed with silica gel which was eluted with CH 2 Cl 2 −MeOH (30:1, v/v) to give a mixture of four macrolactone class of compounds 1−4 . Each of the components was finally purified by reverse-phase HPLC (Inertsil ODS-2, 5 μm, i.d. 4.6×150 mm: solvent system: CH 3 CN−H 2 O = 1:1 (v/v): detection: UV at 275 nm).
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