For various oxidoreductases, the optimum pHs of the enzymes can be calculated using the rule based on proton transfer. Relative probability of a certain amino acid side chain to be in the water, or the relative affinity to the water was calculated using Boltzman distribution. Also, the protonated and deprotonated portions of a certain amino acid side chain were calculated using p$K_R$ of that and the effective protonated and deprotonated protions were the product of relative probability and the protonated and deproteonated protions. Where the total effective protonated portion was equal to the effective deprotonated portion of amino acid side chains, it was expected that oxidoreductases have max-imum activities. The optimum pHs calculated by our rule were compared with the experimental results.
@article{ JCGMDC_2002_v46n5_432}
,title={The Optimum pH of Oxidoreductases: A Comparison Between Experimental and Calculated pH Optimum}
,volume={5}
, url={http://dx.doi.org/10.5012/jkcs.2002.46.5.432}, DOI={10.5012/jkcs.2002.46.5.432}
, number= {5}
, journal={Journal of the Korean Chemical Society}
, publisher={Korean Chemical Society}
, author={Jin Ryeon, Kim
and
Ji Hye, Yang
and
Yeong Je, Yu}
, year={2002}
, month={Oct}
TY - JOUR
T2 - Journal of the Korean Chemical Society
AU - Jin Ryeon, Kim
AU - Ji Hye, Yang
AU - Yeong Je, Yu
SN - 1017-2548
TI - The Optimum pH of Oxidoreductases: A Comparison Between Experimental and Calculated pH Optimum
VL - 46
PB - Korean Chemical Society
DO - 10.5012/jkcs.2002.46.5.432
PY - 2002
UR - http://dx.doi.org/10.5012/jkcs.2002.46.5.432
ER -
Jin Ryeon, K.
,
Ji Hye, Y.
,
&
Yeong Je, Y.
( 2002).
The Optimum pH of Oxidoreductases: A Comparison Between Experimental and Calculated pH Optimum.
Journal of the Korean Chemical Society,
46
(5)
Korean Chemical Society.
doi:10.5012/jkcs.2002.46.5.432
Jin Ryeon, K
,
Ji Hye, Y
,
&
Yeong Je, Y
2002,
The Optimum pH of Oxidoreductases: A Comparison Between Experimental and Calculated pH Optimum,
Journal of the Korean Chemical Society,
vol. 5,
no. 5,
Retrieved from http://dx.doi.org/10.5012/jkcs.2002.46.5.432
[1]
K Jin Ryeon
,
Y Ji Hye
,
and
Y Yeong Je
,
“The Optimum pH of Oxidoreductases: A Comparison Between Experimental and Calculated pH Optimum”,
Journal of the Korean Chemical Society,
vol. 5,
no. 5,
Oct
2002.
Jin Ryeon, Kim
and
,
Ji Hye, Yang
and
,
Yeong Je, Yu
and
,
“The Optimum pH of Oxidoreductases: A Comparison Between Experimental and Calculated pH Optimum”
Journal of the Korean Chemical Society,
5.
5
2002:
Jin Ryeon, K
,
Ji Hye, Y
,
Yeong Je, Y
The Optimum pH of Oxidoreductases: A Comparison Between Experimental and Calculated pH Optimum.
Journal of the Korean Chemical Society
[Internet].
2002.
Oct ;
5
(5)
Available from http://dx.doi.org/10.5012/jkcs.2002.46.5.432
Jin Ryeon, Kim
,
Ji Hye, Yang
,
and
Yeong Je, Yu
,
“The Optimum pH of Oxidoreductases: A Comparison Between Experimental and Calculated pH Optimum.”
Journal of the Korean Chemical Society
5
no.5
()
Oct,
2002):
http://dx.doi.org/10.5012/jkcs.2002.46.5.432