Chiral separation of gemifloxacin, an quinolone antibacterial agent, using (+)-(18-crown-6)-tetracar-boxylic acid $(18C6H_4)$ as a chiral selector was performed by capillary electrophoresis (CE). Direct analysis of quinolone antibacterial agent in body fluid is beneficial in terms of fast analysis time, multicomponent analysis. However, high con-centration of sodium ion in body fluid can prevent gemifloxacin from interacting with $18C6H_4$ since sodium ion has high affinity with $18C6H_4$ due to the strong charge interaction. Ethylenediaminetetraacetic acid (EDTA), as a chelating ligand, was added in the running buffer in order to reduce the interaction between sodium ion and the chiral selector. Increased separation efficiency and reduced migration time were observed while sodium ion exists in the sample solution at the concentration up to 150 mM.
@article{ JCGMDC_2002_v46n5_412}
,title={Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis}
,volume={5}
, url={http://dx.doi.org/10.5012/jkcs.2002.46.5.412}, DOI={10.5012/jkcs.2002.46.5.412}
, number= {5}
, journal={Journal of the Korean Chemical Society}
, publisher={Korean Chemical Society}
, author={Dae Cheon, Gang
and
Seung Il, Jo
and
Du Su, Jeong
and
Gyu Seong, Choe
and
Yong Seong, Kim}
, year={2002}
, month={Oct}
TY - JOUR
T2 - Journal of the Korean Chemical Society
AU - Dae Cheon, Gang
AU - Seung Il, Jo
AU - Du Su, Jeong
AU - Gyu Seong, Choe
AU - Yong Seong, Kim
SN - 1017-2548
TI - Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis
VL - 46
PB - Korean Chemical Society
DO - 10.5012/jkcs.2002.46.5.412
PY - 2002
UR - http://dx.doi.org/10.5012/jkcs.2002.46.5.412
ER -
Dae Cheon, G.
,
Seung Il, J.
,
Du Su, J.
,
Gyu Seong, C.
,
&
Yong Seong, K.
( 2002).
Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis.
Journal of the Korean Chemical Society,
46
(5)
Korean Chemical Society.
doi:10.5012/jkcs.2002.46.5.412
Dae Cheon, G
et al.
2002,
Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis,
Journal of the Korean Chemical Society,
vol. 5,
no. 5,
Retrieved from http://dx.doi.org/10.5012/jkcs.2002.46.5.412
[1]
G Dae Cheon
,
J Seung Il
,
J Du Su
,
C Gyu Seong
,
and
K Yong Seong
,
“Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis”,
Journal of the Korean Chemical Society,
vol. 5,
no. 5,
Oct
2002.
Dae Cheon, Gang
Seung Il, Jo
Du Su, Jeong
et al.
“Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis”
Journal of the Korean Chemical Society,
5.
5
2002:
Dae Cheon, G
,
Seung Il, J
,
Du Su, J
,
Gyu Seong, C
,
Yong Seong, K
Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis.
Journal of the Korean Chemical Society
[Internet].
2002.
Oct ;
5
(5)
Available from http://dx.doi.org/10.5012/jkcs.2002.46.5.412
Dae Cheon, Gang
,
Seung Il, Jo
,
Du Su, Jeong
,
Gyu Seong, Choe
,
and
Yong Seong, Kim
,
“Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis.”
Journal of the Korean Chemical Society
5
no.5
()
Oct,
2002):
http://dx.doi.org/10.5012/jkcs.2002.46.5.412