Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis

Chiral separation of gemifloxacin, an quinolone antibacterial agent, using (+)-(18-crown-6)-tetracar-boxylic acid $(18C6H_4)$ as a chiral selector was performed by capillary electrophoresis (CE). Direct analysis of quinolone antibacterial agent in body fluid is beneficial in terms of fast analysis time, multicomponent analysis. However, high con-centration of sodium ion in body fluid can prevent gemifloxacin from interacting with $18C6H_4$ since sodium ion has high affinity with $18C6H_4$ due to the strong charge interaction. Ethylenediaminetetraacetic acid (EDTA), as a chelating ligand, was added in the running buffer in order to reduce the interaction between sodium ion and the chiral selector. Increased separation efficiency and reduced migration time were observed while sodium ion exists in the sample solution at the concentration up to 150 mM.

Keywords

Capillary Elecrophoresis ; Chiral Separation ; Ethylenediaminetetraacetic Acid ; (+)-(18-crown-6)-tetracar-boxylic Acid

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@article{ JCGMDC_2002_v46n5_412} ,title={Chiral Separation of Quinolone Antibacterial Agent by Capillary Electrophoresis} ,volume={5} , url={http://dx.doi.org/10.5012/jkcs.2002.46.5.412}, DOI={10.5012/jkcs.2002.46.5.412} , number= {5} , journal={Journal of the Korean Chemical Society} , publisher={Korean Chemical Society} , author={Dae Cheon, Gang and Seung Il, Jo and Du Su, Jeong and Gyu Seong, Choe and Yong Seong, Kim} , year={2002} , month={Oct}