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Simultaneous Determination of Tryptophan and Tyrosine by Spectrofluorimetry Using Multivariate Calibration Method
Simultaneous Determination of Tryptophan and Tyrosine by Spectrofluorimetry Using Multivariate Calibration Method
Journal of the Korean Chemical Society. 2002. Aug, 46(4): 309-317
  • Published : August 20, 2002
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Lee, Sang-Hak
Park, Ju-Eun
Son, Beom-Mok

Abstract
A spectrofluorimetric method for the simultaneous determination of amino acids (tryptophan and tyrosine) based on the application of multivariate calibration method such as principal component regression and partial least squares (PLS) to luminescence measurements has been studied. Emission spectra of synthetic mixtures of two amino acids were obtained at excitation wavelength of 257 ��. The calibration model in PCR and PLS was obtained from the spectral data in the range of 280-500 �� for each standard of a calibration set of 32 standards, each containing different amounts of two amino acids. The relative standard error of prediction ($RSEP_a$) was obtained to assess the model goodness in quantifying each analyte in a validation set. The overall relative standard error of prediction ($RSEP_m$) for the mixture obtained from the results of a validation set, formed by 6 independent mixtures was also used to validate the present method.
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