To identify RNA motifs interacting with $tRNA^{Val}$, a SELEX(Systematic Evolution of Ligands by Exponential Enrichment) was applied. Random DNA library which contains a region of ran-domized 48-mer oligonucleotide flanked by conserved sequ ence primers was transcribed into RNA pool using T7 RNA polymerase and RNA aptamers were selected with $tRNA^{Val}$ -immobilized affinity column through 14 rounds of SELEX. Some of the resulting aptamers contained a consensus sequence similar to the sequence in the loop regions of three rRNAs; C43GAAC47 sequence of 5S rRNA, G1491AAGU1495, G1379UUCC1383 sequence of 16S rRNA and C1064UUAG1068, G2110UGUA2114, C2480GACGG2485, A2600CAGU2604 sequence of 23S rRNA. These results suggest that $tRNA^{Val}$ can interact with 5S rRNA, 16S rRNA and 23S rRNA with variety in ribosome.
@article{ JCGMDC_2002_v46n2_157}
,title={In vitro Selection of RNA Aptamers which Bind to Escherichia coli tRNAVal}
,volume={2}
, url={http://dx.doi.org/10.5012/jkcs.2002.46.2.157}, DOI={10.5012/jkcs.2002.46.2.157}
, number= {2}
, journal={Journal of the Korean Chemical Society}
, publisher={Korean Chemical Society}
, author={Bong Rae, Jo}
, year={2002}
, month={Apr}
TY - JOUR
T2 - Journal of the Korean Chemical Society
AU - Bong Rae, Jo
SN - 1017-2548
TI - In vitro Selection of RNA Aptamers which Bind to Escherichia coli tRNAVal
VL - 46
PB - Korean Chemical Society
DO - 10.5012/jkcs.2002.46.2.157
PY - 2002
UR - http://dx.doi.org/10.5012/jkcs.2002.46.2.157
ER -
Bong Rae, J.
( 2002).
In vitro Selection of RNA Aptamers which Bind to Escherichia coli tRNAVal.
Journal of the Korean Chemical Society,
46
(2)
Korean Chemical Society.
doi:10.5012/jkcs.2002.46.2.157
&
Bong Rae, J
2002,
In vitro Selection of RNA Aptamers which Bind to Escherichia coli tRNAVal,
Journal of the Korean Chemical Society,
vol. 2,
no. 2,
Retrieved from http://dx.doi.org/10.5012/jkcs.2002.46.2.157
[1]
and
J Bong Rae
,
“In vitro Selection of RNA Aptamers which Bind to Escherichia coli tRNAVal”,
Journal of the Korean Chemical Society,
vol. 2,
no. 2,
Apr
2002.
Bong Rae, Jo
and
.
“In vitro Selection of RNA Aptamers which Bind to Escherichia coli tRNAVal”
Journal of the Korean Chemical Society,
2.
2
2002:
Bong Rae, J
In vitro Selection of RNA Aptamers which Bind to Escherichia coli tRNAVal.
Journal of the Korean Chemical Society
[Internet].
2002.
Apr ;
2
(2)
Available from http://dx.doi.org/10.5012/jkcs.2002.46.2.157
and
Bong Rae, Jo
.
“In vitro Selection of RNA Aptamers which Bind to Escherichia coli tRNAVal.”
Journal of the Korean Chemical Society
2
no.2
()
Apr,
2002):
http://dx.doi.org/10.5012/jkcs.2002.46.2.157
