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Study on the Structure of 5S rRNA from Pseudomonas alcaligenes by Metallotripeptides
Study on the Structure of 5S rRNA from Pseudomonas alcaligenes by Metallotripeptides
Journal of the Korean Chemical Society. 2002. Feb, 46(1): 46-51
  • Published : February 20, 2002
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Kim, Hee-Joung
Kim, Si-Wouk
Koh, Moon-Joo

Abstract
The recognition and cleavage of 5S rRNA from P. alcaligenes by metallopeptides to the form $Ni(II){\cdot}Gly$-Gly-His(Arg)COOH and $Cu(II){\cdot}Gly$-Gly-His(Arg)COOH were investigated. The results of RNA cleavage analyses suggest that metallopeptides selectively target the unpaired or unstably paired bases of stem-loop structure of 5S rRNA. The selectivity of metallopeptides was little affected by the species of metal ion, Ni(II) or Cu(II). When the result of cleavage by metallopeptides was compared with that of by metal complexes M(II)CR, the recognition by metallopeptides was more selective and structure specific. The cleavage data by metallopeptides and other metal complexes were used to probe the secondary structure of 5S rRNA from P. alcaligenes.
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