We investigated the self-association and protonation state of norfloxacin, a member of quinokme antibiotics, using electric absorption and potentiom,etric titration. Both nitrogen at the piperazine ring and carboxylic acid were protonated at a low pH (cationic norfloxacin), and deprotonated at a high pH (anionic norfloxacin). In the intermediate pH range, a neutral species was dominant with the possibility of forming a zwitter ion. We also observed that nortloxacin molecules can be sracked to form a dimer at an intermediate pH, The equilibrium constant of the norfloxacin-DNA complex formation, which was measured by Stem-Volmermethod, increases as the pH of the system is lowered. This observation indicates that it is the cationic nortloxacin that forms a complex with DNA among various norfloxacin species in aqueous solution.
Citing 'Protonation State of Norfloxacin and Their Interaction with DNA
'
@article{ JCGMDC_2000_v44n1_4}
,title={Protonation State of Norfloxacin and Their Interaction with DNA}
,volume={1}
, number= {1}
, journal={Journal of the Korean Chemical Society}
, publisher={Korean Chemical Society}
, author={Jeong-Ah, Yeo
and
Gwan-Su, Son
and
Jong-Moon, Kim
and
Hyung-Rang, Moon
and
Eun-Duk, Jun
and
Tae-Sub, Cho}
, year={2000}
, month={Feb}
TY - JOUR
T2 - Journal of the Korean Chemical Society
AU - Jeong-Ah, Yeo
AU - Gwan-Su, Son
AU - Jong-Moon, Kim
AU - Hyung-Rang, Moon
AU - Eun-Duk, Jun
AU - Tae-Sub, Cho
SN - 1017-2548
TI - Protonation State of Norfloxacin and Their Interaction with DNA
VL - 44
PB - Korean Chemical Society
PY - 2000
ER -
Jeong-Ah, Y.
,
Gwan-Su, S.
,
Jong-Moon, K.
,
Hyung-Rang, M.
,
Eun-Duk, J.
,
&
Tae-Sub, C.
( 2000).
Protonation State of Norfloxacin and Their Interaction with DNA.
Journal of the Korean Chemical Society,
44
(1)
Korean Chemical Society.
Jeong-Ah, Y
et al.
2000,
Protonation State of Norfloxacin and Their Interaction with DNA,
Journal of the Korean Chemical Society,
vol. 1,
no. 1,
[1]
Y Jeong-Ah
,
S Gwan-Su
,
K Jong-Moon
,
M Hyung-Rang
,
J Eun-Duk
,
and
C Tae-Sub
,
“Protonation State of Norfloxacin and Their Interaction with DNA”,
Journal of the Korean Chemical Society,
vol. 1,
no. 1,
Feb
2000.
Jeong-Ah, Yeo
Gwan-Su, Son
Jong-Moon, Kim
et al.
“Protonation State of Norfloxacin and Their Interaction with DNA”
Journal of the Korean Chemical Society,
1.
1
2000:
Jeong-Ah, Y
,
Gwan-Su, S
,
Jong-Moon, K
,
Hyung-Rang, M
,
Eun-Duk, J
,
Tae-Sub, C
Protonation State of Norfloxacin and Their Interaction with DNA.
Journal of the Korean Chemical Society
[Internet].
2000.
Feb ;
1
(1)
Jeong-Ah, Yeo
,
Gwan-Su, Son
,
Jong-Moon, Kim
,
Hyung-Rang, Moon
,
Eun-Duk, Jun
,
and
Tae-Sub, Cho
,
“Protonation State of Norfloxacin and Their Interaction with DNA.”
Journal of the Korean Chemical Society
1
no.1
()
Feb,
2000):