Ml RNA, the RNA component of RNase P from Escherichia coli, is produced by 3' processing of pre-Ml RNA, a major primary transcript of the rnpB gene. The enzyme fraction containing the processing activity was partially purified and characterized. Since exposure of the active fraction to the high salt condition results in the inactivation of the processing activity, the processing enzyme seems to be an enzyme complex composed of multiple enzymes. The enzyme fraction loses the processing activity when treated with the chemical nuclease lead(II) ion, but regains its activity by the addition of RNA isolated from the enzyme fraction itself, suggesting that an RNA molecule(s) may be essential for the processing activity. Analysis of cleavage sites produced by the partially purified enzyme fraction also implies that the 3' processing occurs by multiple enzymes and at least in two distinct pathways.
Citing 'Partial Purification and Characterization of Enzymes Involved in the Processing of Pre-M1 RNA at the 3' End in Escherichia coli
'
@article{ JCGMDC_1999_v43n3_307}
,title={Partial Purification and Characterization of Enzymes Involved in the Processing of Pre-M1 RNA at the 3' End in Escherichia coli}
,volume={3}
, number= {3}
, journal={Journal of the Korean Chemical Society}
, publisher={Korean Chemical Society}
, author={Ha Dong, Kim
and
Jae Hyeong, Ko
and
Bong Rae, Cho
and
Young Hoon, Lee
and
In Won, Park}
, year={1999}
, month={Jun}
TY - JOUR
T2 - Journal of the Korean Chemical Society
AU - Ha Dong, Kim
AU - Jae Hyeong, Ko
AU - Bong Rae, Cho
AU - Young Hoon, Lee
AU - In Won, Park
SN - 1017-2548
TI - Partial Purification and Characterization of Enzymes Involved in the Processing of Pre-M1 RNA at the 3' End in Escherichia coli
VL - 43
PB - Korean Chemical Society
PY - 1999
ER -
Ha Dong, K.
,
Jae Hyeong, K.
,
Bong Rae, C.
,
Young Hoon, L.
,
&
In Won, P.
( 1999).
Partial Purification and Characterization of Enzymes Involved in the Processing of Pre-M1 RNA at the 3' End in Escherichia coli.
Journal of the Korean Chemical Society,
43
(3)
Korean Chemical Society.
Ha Dong, K
et al.
1999,
Partial Purification and Characterization of Enzymes Involved in the Processing of Pre-M1 RNA at the 3' End in Escherichia coli,
Journal of the Korean Chemical Society,
vol. 3,
no. 3,
[1]
K Ha Dong
,
K Jae Hyeong
,
C Bong Rae
,
L Young Hoon
,
and
P In Won
,
“Partial Purification and Characterization of Enzymes Involved in the Processing of Pre-M1 RNA at the 3' End in Escherichia coli”,
Journal of the Korean Chemical Society,
vol. 3,
no. 3,
Jun
1999.
Ha Dong, Kim
Jae Hyeong, Ko
Bong Rae, Cho
et al.
“Partial Purification and Characterization of Enzymes Involved in the Processing of Pre-M1 RNA at the 3' End in Escherichia coli”
Journal of the Korean Chemical Society,
3.
3
1999:
Ha Dong, K
,
Jae Hyeong, K
,
Bong Rae, C
,
Young Hoon, L
,
In Won, P
Partial Purification and Characterization of Enzymes Involved in the Processing of Pre-M1 RNA at the 3' End in Escherichia coli.
Journal of the Korean Chemical Society
[Internet].
1999.
Jun ;
3
(3)
Ha Dong, Kim
,
Jae Hyeong, Ko
,
Bong Rae, Cho
,
Young Hoon, Lee
,
and
In Won, Park
,
“Partial Purification and Characterization of Enzymes Involved in the Processing of Pre-M1 RNA at the 3' End in Escherichia coli.”
Journal of the Korean Chemical Society
3
no.3
()
Jun,
1999):
