Enzyme electrodes for amperometric measurement of glucose were prepared by immobilization of glucose oxidase on an Immobilon-AV Affinity membrane and attachement to a Pt electrodes. The electrochemical oxidation of Hz02 was monitored at +0.8V vs. Ag/AgCl. Response was linear from 0.2 mM to 20mM. The detection limit was 10m3 mM. Response time, the optimum pH and life time of enzyme immobilized membrane was 12 seconds, pH 5.5(CH3COONaJCH3COOH) and about 27 days, respectively. When the enzyme electrode was applied for the determinaion of glucose with amperometric method, other physiolosical materials have not interfered. Also, we compared the result with that from AOAC(Association of Offical Analytical Chemists) method, measuring the glucose in sweet potato. The relative error was 0.1%.???????????????6?????K????????????
Citing 'Determination of Glucose Using Enzyme Immobilized Membrane
'
@article{ JCGMDC_1999_v43n3_264}
,title={Determination of Glucose Using Enzyme Immobilized Membrane}
,volume={3}
, number= {3}
, journal={Journal of the Korean Chemical Society}
, publisher={Korean Chemical Society}
, author={Im Ok, Kim
and
Gyeong Do, Kwak
and
Yun Sik, Ha
and
Hyo Sik, Kwon
and
Mu Ryong, Seo}
, year={1999}
, month={Jun}
TY - JOUR
T2 - Journal of the Korean Chemical Society
AU - Im Ok, Kim
AU - Gyeong Do, Kwak
AU - Yun Sik, Ha
AU - Hyo Sik, Kwon
AU - Mu Ryong, Seo
SN - 1017-2548
TI - Determination of Glucose Using Enzyme Immobilized Membrane
VL - 43
PB - Korean Chemical Society
PY - 1999
ER -
Im Ok, K.
,
Gyeong Do, K.
,
Yun Sik, H.
,
Hyo Sik, K.
,
&
Mu Ryong, S.
( 1999).
Determination of Glucose Using Enzyme Immobilized Membrane.
Journal of the Korean Chemical Society,
43
(3)
Korean Chemical Society.
Im Ok, K
et al.
1999,
Determination of Glucose Using Enzyme Immobilized Membrane,
Journal of the Korean Chemical Society,
vol. 3,
no. 3,
[1]
K Im Ok
,
K Gyeong Do
,
H Yun Sik
,
K Hyo Sik
,
and
S Mu Ryong
,
“Determination of Glucose Using Enzyme Immobilized Membrane”,
Journal of the Korean Chemical Society,
vol. 3,
no. 3,
Jun
1999.
Im Ok, Kim
Gyeong Do, Kwak
Yun Sik, Ha
et al.
“Determination of Glucose Using Enzyme Immobilized Membrane”
Journal of the Korean Chemical Society,
3.
3
1999:
Im Ok, K
,
Gyeong Do, K
,
Yun Sik, H
,
Hyo Sik, K
,
Mu Ryong, S
Determination of Glucose Using Enzyme Immobilized Membrane.
Journal of the Korean Chemical Society
[Internet].
1999.
Jun ;
3
(3)
Im Ok, Kim
,
Gyeong Do, Kwak
,
Yun Sik, Ha
,
Hyo Sik, Kwon
,
and
Mu Ryong, Seo
,
“Determination of Glucose Using Enzyme Immobilized Membrane.”
Journal of the Korean Chemical Society
3
no.3
()
Jun,
1999):
