GC/MS was used to find an optimum experimental condition for the screening of organic acids in urine. Urinary organic acids were isolated through the liquid-liquid extraction method (LLE) to examine the influence of pH and the effect of including the back extraction and oximation processes respectively on the extraction. When pH was adjusted to 0.5 during the extraction without oximation process, relatively higher recovery rate and the smallest relative standard deviations (0.3-12.4%) were obtained. By removing the interference, the addition of back extraction made possible surer identification of organic acids with retention time of 15-16 minutes. Under this condition, we obtained the content distribution of urinary organic acids in healthy Korean children (n=16) by establishing the calibration curves for 51 standard organic acids.
Citing 'Analysis of Organic Acids in Urine by Gas Chromatography/Mass Spectrometry
'
@article{ JCGMDC_1997_v41n9_471}
,title={Analysis of Organic Acids in Urine by Gas Chromatography/Mass Spectrometry}
,volume={9}
, number= {9}
, journal={Journal of the Korean Chemical Society}
, publisher={Korean Chemical Society}
, author={Eun Ah, Yoo
and
Sun Young, Ko
and
Jong Won, Kim
and
Jeong Uk, Kim
and
Seung Woon, Myung}
, year={1997}
, month={Sep}
TY - JOUR
T2 - Journal of the Korean Chemical Society
AU - Eun Ah, Yoo
AU - Sun Young, Ko
AU - Jong Won, Kim
AU - Jeong Uk, Kim
AU - Seung Woon, Myung
SN - 1017-2548
TI - Analysis of Organic Acids in Urine by Gas Chromatography/Mass Spectrometry
VL - 41
PB - Korean Chemical Society
PY - 1997
ER -
Eun Ah, Y.
,
Sun Young, K.
,
Jong Won, K.
,
Jeong Uk, K.
,
&
Seung Woon, M.
( 1997).
Analysis of Organic Acids in Urine by Gas Chromatography/Mass Spectrometry.
Journal of the Korean Chemical Society,
41
(9)
Korean Chemical Society.
Eun Ah, Y
et al.
1997,
Analysis of Organic Acids in Urine by Gas Chromatography/Mass Spectrometry,
Journal of the Korean Chemical Society,
vol. 9,
no. 9,
[1]
Y Eun Ah
,
K Sun Young
,
K Jong Won
,
K Jeong Uk
,
and
M Seung Woon
,
“Analysis of Organic Acids in Urine by Gas Chromatography/Mass Spectrometry”,
Journal of the Korean Chemical Society,
vol. 9,
no. 9,
Sep
1997.
Eun Ah, Yoo
Sun Young, Ko
Jong Won, Kim
et al.
“Analysis of Organic Acids in Urine by Gas Chromatography/Mass Spectrometry”
Journal of the Korean Chemical Society,
9.
9
1997:
Eun Ah, Y
,
Sun Young, K
,
Jong Won, K
,
Jeong Uk, K
,
Seung Woon, M
Analysis of Organic Acids in Urine by Gas Chromatography/Mass Spectrometry.
Journal of the Korean Chemical Society
[Internet].
1997.
Sep ;
9
(9)
Eun Ah, Yoo
,
Sun Young, Ko
,
Jong Won, Kim
,
Jeong Uk, Kim
,
and
Seung Woon, Myung
,
“Analysis of Organic Acids in Urine by Gas Chromatography/Mass Spectrometry.”
Journal of the Korean Chemical Society
9
no.9
()
Sep,
1997):
