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MALDI Mass Spectrometric Analysis of Nonderivatized Steroids Using Cyclodextrin-supported 2,5-Dihydroxybenzoic Acid as Matrix
MALDI Mass Spectrometric Analysis of Nonderivatized Steroids Using Cyclodextrin-supported 2,5-Dihydroxybenzoic Acid as Matrix
Bulletin of the Korean Chemical Society. 2014. May, 35(5): 1409-1412
Copyright © 2014, Korea Chemical Society
  • Received : December 04, 2013
  • Accepted : January 20, 2014
  • Published : May 20, 2014
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About the Authors
Jeongjin Son
Sangwon Cha

Abstract
Sex hormones are important metabolites in vertebrates’ development and reproduction. For rapid screening sex hormones, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is one of the promising analytical platforms, but MALDI MS faces many challenges in detecting steroids such as low ionization efficiency and matrix background interference. One potential strategy to overcome matrix interference in the low m/z region is using a cyclodextrin (CD)-supported matrix for steroid analysis since CD-supported matrixes are known to effectively suppress matrix-related ion signals. In this study, we aimed to find the optimal CD-supported matrix for the analysis of the nonderivatized sex steroids. Our results showed that the αCD-supported 2,5-dihydroxybenzoic acid (DHB) matrix efficiently ionized all three major classes of sex hormones, estrogens, androgens, and progestagens, with low or no matrix background and also with high sensitivity. In addition, the αCD-supported DHB matrix mainly generated molecular ions or protonated ions of sex hormones, and this enabled us to obtain information-rich tandem mass spectra which potentially lead to unambiguous identification of steroid species from complex metabolite mixtures.
Keywords
Introduction
Sex hormones are essential biomolecules in vertebrates and mainly involved in the maintenance of pregnancy, the development of secondary sex characteristics, and many diseases such as osteoporosis and breast cancer. There are three major classes of sex steroids, estrogens, androgens, and progestagens which have 18, 19, and 21 carbon atoms in their core structures, respectively.
In the analysis of sex hormones, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is a very attractive method because of its ability to analyze complex mixtures with a simple sample preparation protocol and also because of its potential as a high throughput screen-ing platform. However, MALDI MS of neutral steroids is challenging with following reasons. First, ion yields of steroids in MALDI MS are usually very low since neutral steroids lack acidic or basic groups in their structures and this leads to poor sensitivity. Second, intense MALDI matrix ion signals in the low m/z region often interfere with or suppress steroid signals.
In order to overcome these issues, many research efforts have been made so far. First, steroids can be derivatized to improve ion yields of them. For example, 3-oxo-sterols were derivatized with Girad P hydrazine to give charged Girad P hydrazones, and these derivatized steroids were easily detected as molecular ions ([M] + ) by conventional MALDI MS. 1 In addition, these derivatized oxysterols also generated information-rich high energy collision induced dissociation (CID) tandem mass spectra.
Second, a high molecular weight matrix can be used to avoid the overwhelmed matrix ion signals in the low m/z region. For example, a meso-tetrakis(pentafluorophenyl)-porphyrin (F20TPP, MW 974.57) matrix was used for the analysis of the five neutral steroids from human urine. 2 Third, a surface-assisted laser desorption/ionization (SALDI) MS platform or an alternative matrix such as nanomaterials can be employed to both improve ion yield and avoid matrix interference. For example, an activated carbon surface and C 70 fullerene was used for the analysis of the nonderivatized steroids. 3 4 Interestingly, with a carbon-based matrix, estro-gens were more effectively detected in the negative ion mode while testosterone and progesterone were preferential-ly ionized in the positive ion mode. 3 Gold nanoparticles were also used as a matrix for the detection of testosterone and progesterone. 5
A cyclodextrin (CD)-supported matrix, originally develop-ed by Korenaga and co-workers, is another effective way to reduce matrix interference. 6 - 8 A CD-supported matrix is a complex of CD and an organic MALDI matrix prepared by simple heated sonication, and their complex formation was confirmed by UV-VIS and 1 H NMR spectroscopy. 6 7 With a CD-supported matrix, matrix-related ions were significantly suppressed and therefore much cleaner mass spectra could be obtained for various small molecules from forensic samples. 8
In present study, we developed a MALDI MS method for the analysis of the nonderivatized sex steroids by optimizing a CD-supported matrix system. We found that all major classes of sex hormones (Figure 1) were detected by MALDI with a clean background when using the αCD-supported 2,5-dihydroxybenzoic acid (DHB) matrix. In addition, we also confirmed that the αCD-supported DHB is a suitable matrix for producing information-rich product ion spectra of neutral steroids.
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Chemical structures of sex hormones analyzed in this study: (a) estrone, (b) β−estradiol, (c) testosterone, and (d) pro-gesterone.
Experimental
Materials. Estrone (MW 270.37), β-estradiol (MW 272.38), testosterone (MW 288.42), progesterone (MW 314.46), tri-fluoroacetic acid (TFA, 99% purity), 2,4,6-trihydroxyaceto-phenone (THAP), and cyclodextrins (α-, β-, and 𝛾- forms) were obtained from Sigma-Aldrich (St. Louise, MO, USA). A recrystallized DHB was purchased from Protea Biosci-ences (Morgantown, WV, USA). Aqueous solution of citrate-capped gold nanoparticles (0.05 mg/mL, 12.0 ± 1.3 nm in diameter) was purchased from CNVision Co. (Seoul, Korea). All solvents except deionized water were obtained from Fisher Scientific (Fairlawn, NJ, USA).
Sample Preparation for MALDI MS Analysis. The standard solutions of steroids were prepared in ethanol with the concentration of 1.0 mg/mL. The αCD-supported DHB matrix solution was prepared based on a previous protocol 8 with some modification. Briefly, 10 mM αCD solution in water and DHB solution (20 mg/mL) in 50/50 acetonitrile/ water were prepared separately. Then, 20 µL of DHB solution was mixed with 120 µL of αCD solution, and the mixture was sonicated for 10 min at 50 ℃. In case of the gold nanoparticle matrix, the solution obtained from CNVision Co. was directly used without further treatment.
For MALDI MS analysis, a steroid sample solution (0.5 µL) was first spotted onto a 384-well MALDI target plate (ASTA Inc., Suwon, Korea), followed by 0.5 µL matrix solution. After spotting, spotted samples were dried under a moderate vacuum (~50 Torr) at room temperature.
MALDI Mass Spectrometry. ABI 4800 Plus MALDI-TOF/ TOF analyzer (Applied Biosystems, Foster City, CA) controlled by the 4000 Series Explorer TM software was used for MALDI MS and MS/MS analyses of steroids. The mass spectrometer was operated in the positive reflectron mode with a 20 kV acceleration voltage. For a given sample spot, sub-spectra were collected from 20 different locations in the spot and then averaged to generate a final mass spectrum (800 laser shots in total per sample spot). In case of MS/MS experiments, high energy CID was performed using air as the collision gas at 1 kV of collision energy. A DataExplorer V4.8 (Applied Biosystems) and a mMass software (http://www.mmass.org) 9 were used for further processing mass spectra.
Results and Discussion
Optimization of a CD-Supported Matrix for Detection of Nonderivatized Steroids. We initially tested the previ-ously reported αCD-supported THAP matrix for the steroid analysis. 7
Among steroids investigated, testosterone and proge-sterone were efficiently ionized by the αCD-supported THAP matrix with minimal matrix interference similar to the previous study. 8
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MALDI mass spectra of estrone (a) with the DHB-only matrix, (b) with the αCD-supported DHB matrix prepared by heated sonication for 10 min, and (c) with the DHB plus αCD matrix prepared by 5 min vortexing. The peak at m/z 287 in (a) is an unknown peak coming from either a DHB matrix or a MALDI target plate. BPI stands for absolute base peak intensity.
However, 18–carbon sex hormones, estrogens (estrone and β–estradiol) were not ionized by the αCD-supported THAP matrix or by the THAP-only matrix. Therefore, we tested other organic MALDI matrixes in order to find a suitable matrix for ionization of sex hormones.
Among matrixes tested, DHB showed an acceptable per-formance in detecting estrogens as shown in Figure 2(a) . With a DHB-only matrix, estrone was detected as M + at m/z 270, [M + H] + at m/z 271, [M + Na] + at m/z 293, and [M + K] + at m/z 309, but the mass spectrum was dominated by ion signals originated from a DHB matrix. In order to achieve suppression of matrix-related signals, various sizes of CDs (α-, β-, and 𝛾- forms) were incorporated into the DHB matrix and tested.
Among CD-DHB complexes investigated, only αCD-DHB showed a matrix suppression effect with detectible steroid ion signals, and therefore further optimization in matrix preparation was carried out with αCD only. In the previous study, the optimized molar ratio of THAP and αCD was 1:1 and the concentration was ~8.8 mM each in the final matrix solution. 6 7 However, the αCD-supported DHB matrix pre-pared from the equimolar mixture of DHB and αCD (~8.8 mM, respectively) did not ionize steroids efficiently although this matrix showed good matrix suppression. Therefore, we generated matrix solutions with various molar ratios of DHB and αCD ranging from 10:1 to 1:100 (DHB: αCD), and tested these solutions against steroids. From this investi-gation, we found that a molar ratio of near 2:1 (DHB:αCD, 18.5 mM:8.6 mM) produced the best result. Further investi-gation is needed to clearly understand complex formation or host-guest interaction between DHB and αCD.
Figure 2(b) shows the MALDI mass spectrum of estrone with the optimized αCD-supported DHB matrix. Compared to Figure 2(a) , ion signals from DHB were clearly suppress-ed while estrone ion signals were enhanced. It should be also noted that the αCD-supported DHB matrix generated mainly molecular ions or protonated ions which is preferred in structural analysis of steroids by MS/MS. This will be discussed in more detail in the next chapter.
In addition, we examined whether heated sonication is also required for the preparation of the αCD-supported DHB matrix, and we confirmed that the αCD-supported DHB matrix prepared by heated sonication gave the better sup-pression of matrix ion signals as well as the more enhance-ment of steroid signals than one prepared by the simple vigorous mixing as shown in Figure 2(b) and 2(c) . At least 5 min sonication at 50 ℃ was required for the effective formation of αCD-DHB complex.
Figure 3 shows MALDI mass spectra of other sex steroids with the optimized αCD-supported DHB matrix. All mass spectra showed molecular or protonated ions of steroids as a major species with low or no matrix-related ion signals. The limits of detection (S/N > 5) of estrone and β–estradiol were 50 pmol per sample spot and the limits of detection (S/N > 5) of progesterone and testosterone were 2 pmol per sample spot. These results were comparable or better than those obtained by carbon material- or gold nanoparticle-based LDI MS methods. 3 - 5
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MALDI mass spectra of nonderivatized sex hormones with the αCD-supported DHB matrix: (a) β–estradiol, (b) testo-sterone, and (c) progesterone. BPI stands for absolute base peak intensity.
Structural Analysis of Nonderivatized Steroids by αCD-supported Matrix-based MALDI MS/MS. In previous reports, laser desorption/ionization (LDI) MS methods using an activated carbon surface or gold nanoparticle as a matrix mainly generated sodium adduct ions of sex steroids in the positive ion mode. 4 5 This alkali cationization of steroids is not a problem when it is possible to identify steroids only by their m/z values. However, in case of analyzing a complex steroid mixture, identification of steroid species only by their m/z values is not feasible since many isobaric steroids or other small metabolites can be present in a mixture. There-fore, structural analysis by MS/MS is usually required and performed. In such case, alkali cationized ions are potenti-ally problematic because these ions do not often provide information-rich MS/MS due to their less covalent nature in ion formation.
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(a) LDI mass spectrum of estrone with an aqueous solution of gold nanoparticles as a matrix. (b) MALDI TOF/TOF mass spectrum of [estrone + Na]+ ion detected at m/z 293 in (a). (c) MALDI TOF/TOF mass spectrum of [estrone]+ and [estrone + H]+ ions detected at m/z 270 and 271 in the MALDI mass spectrum of estrone with the αCD-supported DHB matrix (Figure 2(b)). Chemical structures in the spectrum are possible fragment ions from [estrone + H]+ based on previous studies.110-12
Figure 4(a) shows the MALDI mass spectrum of estrone with an aqueous solution of gold nanoparticles as a matrix. Similar to the previous report, 5 estrone was exclusively and easily detected as a [M + Na] + form. In MS/MS of this ion, however, almost no fragment ion except sodium ion was observed ( Figure 4(b) ). In contrast, the product ion spectrum of the molecular ion and the protonated ion appeared in the MALDI mass spectrum of estrone with the αCD-supported DHB matrix ( Figure 2(b) ) provided detailed structural information as illustrated in Figure 4(c) . Chemical structures presented in Figure 4(c) are possible product ions of pro-tonated estrone ions. 1 10 - 12 This suggests that αCD-supported DHB MALDI MS has a potential advantage over other SALDI MS methods in structural analysis of neutral steroids and also in complex steroid mixture analysis.
Conclusion
In this study, we optimized a CD-supported matrix for the detection of the nonderivatized steroids by MALDI MS. By using the αCD-supported DHB matrix, all three major classes of sex steroids were detected with high sensitivity while background ions originated from a DHB matrix were effec-tively suppressed. In addition, since major ion forms of steroids produced by the αCD-supported DHB matrix were molecular or protonated ions, informative fragmentation patterns could be obtained by high energy CID MS/MS which is a potential benefit in complex steroid mixture analysis. Future studies will include the application of the method to the steroid analysis of complex biological fluids or tissues, the investigation of the αCD-DHB interaction properties, and the further optimization of the method for improving sensitivity.
Acknowledgements
This work was supported by Hankuk University of Foreign Studies Research Fund of 2013.
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