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Synthesis of Hapten for Indirect Competitive Immunoassay for Measuring 3,5,6-trichloro-2-pyridinol
Synthesis of Hapten for Indirect Competitive Immunoassay for Measuring 3,5,6-trichloro-2-pyridinol
Korean Journal of Environmental Agriculture. 2015. Dec, 34(4): 345-349
Copyright © 2015, The Korean Society of Environmental Agriculture
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • Received : September 10, 2015
  • Accepted : October 24, 2015
  • Published : December 31, 2015
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About the Authors
Areumnuri Kim
Chemical Safety Division, National Institute of Agricultural Sciences, Wanju 55365, Korea
Joong-Young Kim
BIONAST Co., Ltd., Seongnam 13216, Korea
Sang-Hee Jeong
Department of Applied Biotoxicology, Hoseo University, Asan 31499, Korea
Myung-Haing Cho
College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea
Kyung-Hun Park
Chemical Safety Division, National Institute of Agricultural Sciences, Wanju 55365, Korea
Namjun Cho
Chemical Safety Division, National Institute of Agricultural Sciences, Wanju 55365, Korea
Min Kyoung Paik
Chemical Safety Division, National Institute of Agricultural Sciences, Wanju 55365, Korea
mink1114@korea.kr

Abstract
BACKGROUND:
In this study, we have attempted to identify a urinary biomarker to assess chlorpyrifos exposure in farmers. The major metabolite and the excretion pathway of chlorpyrifos is 3,5,6-trichloro-2-pyridinol (TCP) in urine. Herein, we describe an adequate synthetic method for TCP hapten for measuring urinary TCP of farmers.
METHODS AND RESULTS:
First, TCP was prepared by spacer attachment through hydrolysis of thiophosphate ester from chlorpyrifos. After reaction with benzyl bromide, the TCP was transformed into 2,3,5-trichloro-6-benzyloxypyridine. Next, the chlorine in the 2 nd position of the pyridyl ring was substituted into 3-mercaptopropanoic acid spacer arm. Finally, the phenyl group attached to the 6 th position in pyridyl ring was removed for producing the targeted product, 3-(3,5-Dichloro-6-hydroxy-2-pyridyl) thiopropanoic acid.
CONCLUSION:
Henceforth, this TCP hapten would be used in developing immunoassay studies for the detection and quantitation of urinary TCP of farmers.
Keywords
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Acknowledgements
This work is supported by “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ00995001)” Rural Development Administration, Republic of Korea.
References
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