Effects of Lycium chinense Miller Fruit and its Constituent Betaine on Immunomodulation in Balb/c Mice
Effects of Lycium chinense Miller Fruit and its Constituent Betaine on Immunomodulation in Balb/c Mice
Korean Journal of Environmental Agriculture. 2014. Sep, 33(3): 189-193
Copyright © 2014, The Korean Society of Environmental Agriculture
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • Received : September 15, 2014
  • Accepted : September 25, 2014
  • Published : September 30, 2014
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About the Authors
Na-Hyung, Kim
Hanbang Body-Fluid Research Center & College of Oriental Medicine, Wonkwang University, Shinyong-Dong, Iksan 570-749, Korea
Seung-Hwa, Baek
Department of Biofood Science and Technology, Chungbuk Provincial University, Okcheon, Chungbuk, 373-800, Korea

The aim of this study is to investigate the effects of Lycium chinense Miller fruit and its representative constituent betaine on reduction of immobility time and blood parameters in balb/c mice.
We investigated the immobility time and the changes in aspects of blood biochemical parameters by the administration of L. chinense Miller fruit and its representative constituent betaine, after the forced swimming test. The immobility time was significantly reduced about 41.3% and 53.6%, respectively, in the animal of L. chinense Miller fruit and its representative constituent betaine-administrated group for 7 days, in comparison with that of the control group. The level of blood urea nitrogen was significantly decreased in L. chinense Miller fruit and its representative constituent betaine-treated group compared with the control group ( P < 0.05), respectively. In addition, the interlukin-2 levels of mice in L. chinense Miller fruit and betaine treated group was increased compared with the control group.
These results indicate that L. chinense Miller fruit and betaine might be helpful in the immune function improvement, enhance physical stamina, and fatigue recovery.
Lycium chinense Miller fruit (LF) is distributed in warm and subtropical regions of southeastern Asia and European countries, and it has been used as an agriculture source for anti-aging or hepatoprotective antioxidant ( Ahn ., 2014 ). A representative component of LF, betaine has reported that it is the natural amino acid and involved in the synthesis of methionine from homocysteine in the liver as an oxidative metabolite of choline ( Zhao ., 2013 ; Ahn ., 2014 ). In addition, betaine exerts various physiologic functions such as anti-atherosclerosis, anti-osteoporosis and protective effect against chemical liver injury ( Zhao , 2013 ).
Forced swimming test, which is a behavioral test for rodents to predict the efficacy of antidepressant treatments, is recently used to examine whether certain agents have an anti-fatigue effect ( Porsolt ., 1977 ; Moriura ., 1996 ). Fatigue, defined as a loss of force-generating capacity, may develop for a variety of reasons and involve both central and peripheral factors ( Kim , 2008 ). The feeling of fatigue is also recognized in chronic medical illness including an inmate of a hospital in intensive care unit or enteral/parenteral feeding condition ( Kim ., 2008 ). Forced swimming test causes alterations in cellular and non-cellular immunity, lowers the ratio of lymphocytes, enhances the ratio of neutrophils in rat peripheral blood ( Dubovik and Bogomazov, 1987 ; Delbende ., 1994 ; Connor ., 1997 ; Kim ., 2010 ). In addition, Shin et al. (2004) have demonstrated on a relationship between immune function and immobility time after a forced swimming test by ICR mice. They have been made an effort in the development and research of natural products with those health benefits in many countries. The aim of this study was to investigate the effects of LF and its representative constituent betaine on reduction of immobility time, changes of blood parameters and the release of cytokines such as interferon (IFN)-γ and interlukin (IL)-2 in balb/c mice.
Materials and Methods
LF was purchased from Kyungdong traditional herb market (Seoul, Korea). For the preparation of LF, the 20 g of crude LF were washed and air-dried. An extract of LF was prepared by decocting with boiling distilled water and the duration of decoction was about 3 h. The decoction was filtered, lyophilized and kept at 4C. The yield of dried extract from initial crude materials for the study was about 8.2%. After the samples were dissolved in saline, they were filtered through a 0.45 μm syringe filter and kept at 4C. For LF or betaine treated group, it was dissolved in distilled water and administrated at dose of 10 mg/kg/day and 0.1 mg/kg/day for the study, respectively, once per day for 7 days using a feeding atraumatic needle. Fluoxetine (10 mg/kg/day) was used as a positive control of this study.
Betaine, avidine-peroxidase, and 2′-AZINO-bis(3-ethylbenzithiazoline-sullfonic acid) tablets substrate (ABTS) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Anti-human IFN-γ and IL-2 monoclonal antibodies, biotinylated anti-human IFN-γ and IL-2 and recombinant IFN-γ and IL-2 were purchased from R&D Systems (Minneapolis, MN, USA). Male balb/c mice (5-week-old, 19-21 g) were purchased from the Da-Mul Experimental Animal Center (Daejon, Korea). The animals were housed four to ten per cage in a laminar air-flow room maintained at a temperature of 22 ± 1℃, relative humidity of 55 ± 10%, 12:12 L/D cycle and light on at 07:00 h throughout the study. Food and water were available ad libitum . All manipulations were carried out between 09:00 and 16:00 h, and no animal was used more than once. The experiment was approved by the institutional review board and animal ethics committee (No. wku12-28) and conducted by in accordance with the internationally accepted principles for laboratory animal use and care as found in the US guidelines (NIH publication #85-23, revised in 1985).
During the 6 min of the forced swimming test, the duration of immobility was measured as previously described by Porsolt et al . (1977). The apparatus consisted of two Plexiglas cylinders (height: 25 cm, diameter: 10 cm) placed side by side in a Makrolon cage filled with water (10 cm height) at 23-25℃. Two mice were tested simultaneously for a 6 min period inside vertical Plexiglas cylinders; a nontransparent screen placed between the two cylinders prevented the mice from seeing each other. The total duration of immobility, after a delay of 2 min, was measured during a period of 4 min. Each mouse was considered to be immobile when it ceased struggling and remained floating motionless in the water, making only those movements necessary to keep its head above water. After the last forced swimming test, mice were anesthetized with an intraperitoneal injection of ketamine (80 mg/Kg) and xylazine (4 mg/Kg) and blood (1 mL) was withdrawn from the heart of mice into syringes. Then, blood was prepared by centrifugation at 1500 × g , 4℃ for 10 min. Contents of blood urea nitrogen (BUN), creatine kinase (CK), lactate dehydrogenase (LDH), glucose, total protein, and albumin were determined by an autoanalyzer (Hitachi 747, Hitachi, Japan). Additionally, enzyme-linked immunosorbent assay (ELISA) was carried out for IFN-γ and IL-2 of serum in mice of experimental groups in duplicate using a 96-well format (Nunc, Denmark). After treating each antibodies and washing the wells, avidin-peroxidase was added and plates were incubated for 20 min at 37C. Then, ABTS substrate was added. Color development was measured at 405 nm using an automated microplate ELISA reader. All results were statistically analyzed by SPSS 19.0 analysis program and presented as mean ± standard error of mean (SEM). Data were calculated by using student t-test, one-way analysis of variance and the post hoc test was performed via the Turkey′s test. p -values of less than 0.05 were used as the criterion for statistical significance.
Results and Discussion
The immobility time of mice in control group, fluoxetine group, LF group, and betaine group was 138.5 s, 73.8 s, 81.3 s, and 64.3 s, respectively ( Fig. 1 ). Reduction of the immobility time during forced swimming test traditionally interpreted as a measure of ‘behavioral despair’ or ‘learned helplessness’ ( Gao, 2014 ). Many studies show the possibility that retrenchment the amount of time an animal spends immobile in the forced swimming test by natural compounds have the relation with cytokines and blood parameters ( Shin ., 2004 ; Kim ., 2008 ; Kim ., 2010 ) However, it was not illuminated yet detail mechanism on its relation between immobility time and change of cytokines/ blood parameters. The immobility time of mice in fluoxetine administrated groups, LF administrated groups, and betaine administrated groups reduced significantly at the last day, compared with saline administrated group ( P < 0.05). These results indicated that administration of LF and betain improve the body activity and decrease behavioral despair.
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Effect of LF and betaine on immobility time in mouse forced swimming test. Values are means ± SEM. *P < 0.05 vs. significantly different from CON. CON, saline-administrated control group; Flu, fluoxetine (10 mg/ kg/day, p.o.)-administrated group; LF, Lycium chinense Miller fruit (10 mg/ kg/day, p.o.)-administrated group; Bot, Botaine (0.1 mg/ kg/day, p.o.)-administrated group.
Previous studies demonstrated that the swimming exercise is to induce the biochemical changes in blood ( De‐Mello , 1992 ). It was investigated in the changes of blood parameters such as BUN, CK, LDH, glucose, total protein, and albumin ( Fig. 2 ). The contents of BUN of mice in fluoxetine, LF, and betaine treated groups were 20.9, 21.1, and 20.9 mg/dL, respectively, and decreased significantly in comparison with that in saline treated control group ( P < 0.05). The CK and LDH levels of saline, fluoxetine, LF, and betaine treated group did not represent significant difference compared with a control group, although they had tends to decrease. Additionally, the value of glucose, total protein, and albumin had no effect in LF and betaine treated groups, compared with a control group. The level of BUN is standard metrics used to diagnose and monitor kidney injury ( Ferquson , 2000 ). CK and LDH are generally known accurate indicators of muscle damage ( Coombes and McNaughton, 2000 ). Glucose plays an important role as main substrates for energy during exercise or starvation ( Rose and Sampson, 1982 ). The value of total protein is known as an indicator of nutritional state, kidney disease and chronic liver disease ( Costill and Fink 1974 ). Albumin level indicates also immune or nutrition states in the body ( Whicher and Spence 1987 ). Thus, the reduction of BUN values by LF and betaine indicated that they could have a play in alleviating kidney injury by forced swimming test.
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Concentration of BUN, CK, LDH, glucose, total protein and albumin after forced swimming test. Values are means ± SEM. *P <0.05 vs. significantly different from CON. CON, saline-administrated control group; Flu, fluoxetine (10 mg/ kg/day, p.o.)-administrated group; LF, Lycium chinense Miller fruit (10 mg/ kg/day, p.o.)-administrated group; Bot, Botaine (0.1 mg/ kg/day, p.o.)-administrated group.
Immunoregulatory cytokines play an important role in immune response ( Bernabei ., 2003 ; Kim , 2010 ). Of these immunoregulartory cytokines, they are known that IFN-γ produced by T and natural killer cells is considered the principal effector cytokine of cell-mediated immunity, and IL-2 is a T cell growth factor, can augment natural killer cell cytolytic activity, or promotes immunoglobulin production by B cells ( Kim ., 2006 ; Lenardo, 1991 ). It was evaluated that whether LF and its constituent betaine have enhanced the IFN-γ and IL-2 levels in blood of mice after forced swimming test compared with control groups ( Table 1 ). The levels of IL-2 in betaine treated cells were significantly increased compared to that of control ( P < 0.05), and IFN-γ levels also tends to increase by LF and betaine treatment, respectively. The contents of released IL-2 from mice of each group in the saline, fluoxetine, LF, betaine treated groups was 112.3, 113.5, 134.3, and 143.0 pg/mL, respectively. The oral administration of fluoxetine and LF during 7 days showed no significant results compared with control group, but tended to increase IFN-γ and IL-2 levels by LF. These results show that it regulates IL-2 levels which reduced after forced swimming test, by its constituent betaine than LF itself.
Body weight and the production of IFN-γ and IL-2
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CON, saline-administrated control group; Flu, fluoxetine (10 mg/kg/day, p.o.)-administrated group; LF, Lycium chinense Miller fruit (10 mg/ kg/day, p.o.)-administrated group; Bot, Botaine (0.1 mg/ kg/day, p.o.)-administrated group. Values represent the mean SEM. *P < 0.05 vs. significantly different from CON.
Finally, it is shown that LF and betaine decrease the immobility time of mice in forced swimming test and tend to decrease BUN or enhance the level of IFN-γ and IL-2 levels. From this, it is indicated that LF and betaine could have the possibility as a useful agriculture and herbal source in the immune function improvement, enhance physical stamina, and fatigue recovery. However, it needs the further research on approach more detailed mechanisms of the relationship between the immobility time and cytokine production in mice model.
Present results provide evidences that the administration of LF and its constituent betaine decrease the immobility times after a forced swimming test in mice. Moreover, this treatment tends to improve the levels of several blood biochemical parameters, after a forced swimming test. In addition, the levels of IFN-γ and IL-2 tend to enhance by LF and betaine treatments. These results indicate that LF and betaine might be helpful in the immune function improvement, enhance physical stamina, and fatigue recovery.
Ahn M. , Park J.S. , Chae S. , Kim S. , Moon C. , Hyun J.W. , Shin T 2014 Hepatoprotective effects of Lycium chinense Miller fruit and its constituent betaine in CCl4-induced hepatic damage in rats Acta. Histochem 116 1104 - 1112
Bernabei P. , Bosticardo M. , Losana G. , Di Paola F. , De Angelis S. , Giovarelli M. , Novelli F. 2003 IGF-1 down-regulates IFN-gamma R2 chain surface expression and desensitizes IFN-gamma/STAT-1 signaling in human T lymphocytes Blood 102 2933 - 2939
Connor T.J. , Kelly J.P. , Leonard B.E 1997 Forced swim test-induced neurochemical endocrine, and immune changes in the rat Pharmacol. Biochem. Behav. 58 961 - 967
Coombes J.S. , McNaughton L.R. 2000 Effects of branched-chain amino acid supplementation on serum creatine kinase and lactate dehydrogenase after prolonged exercise J. Sports Med. Phys. Fitness 40 240 - 246
Costill D.L. , Fink W.J. 1974 Plasma volume changes following exercise and thermal dehydration J. Appl. Physiol 37 521 - 525
Delbende C. , Bunel D. , Tarozzo G. , Grino M. , Oliver C. , Mocaer E. , Vaudry H. 1994 Effect of chronic treatment with the antidepressant tianeptine on the hypothalamo-pituitary-adrenal axis Eur. J. Pharmacol 251 245 - 251
De-Mello M.A 1992 Effect of exercise during pregnancy and dam age on maternal blood chemistry and fetal growth Braz. J. Med. Biol. Res. 25 537 - 542
Dubovik B.V. , Bogomazov S.D 1987 Multifactorial method for assessing the physical work capacity of mice Farmakol. Toksikol 50 116 - 121
Ferquson M.A. , Vaidya V.S. , Bonventre J.V 2008 Biomarkers of nephrotoxic acute kidney injury Toxicology 245 182 - 193
Gao V. , Vitaterna M.H. , Turek F.W. 2014 Validation of video motion-detection scoring of forced swim test in mice J. Neurosci. Methods 235 59 - 64
Kim H.P. , Imbert J. , Leonard W.J. 2006 Both integrated and differential regulation of components of the IL-2/IL-2 receptor system Cytokine Growth Factor Rev. 17 349 - 366
Kim N.H. , Kim K.Y. , Jeong H.J. , Kim H.M. , Hong S.H. , Um J.Y. 2010 Effects of hydrolyzed Chlorella vulgaris by malted barley on the immunomodulatory response in ICR mice and in Molt-4 cells J. Sci. Food Agric. 90 1551 - 1556
Kim N.H. , Jeong H.J. , Lee J.Y. , Go H. , Ko S.G. , Hong S.H. , Kim H.M. , Um J.Y. 2008 The effect of hydrolyzed Spirulina by malted barley on forced swimming test in ICR mice Int. J. Neurosci 118 1523 - 1533
Lenardo M.J. 1991 Interleukin-2 programs mouse alpha beta T lymphocytes for apoptosis Nature 353 858 - 861
Moriura T. , Matsuda H. , Kubo M 1996 Pharmacological study on Agkistrodon blomhoffii BOIE.V. antifatigue effect of the 50% ethanol extract in acute weight-loaded forced swimming-treated rats Biol. Pharm. Bull. 19 62 - 66
Porsolt R.D. , Bertin A. , Jalfre M 1977 Behavioral despair in mice: a primary screening test for antidepressants Arch. Int. Pharmacodyn. Ther. 229 327 - 336
Rose R.J. , Sampson D 1982 Changes in certain metabolic parameters in horses associated with food deprivation and endurance exercise Res. Vet. Sci. 32 198 - 202
Shin H.Y. , Shin T.Y. , Seo S.W. , Na H.J. , Kwon Y.T. , Song B.K. , Lee E.J. , Kim Y.K. , Hong M.C. , Shin M.K. , Hong S.H. , Kim H.M. 2004 Decrease of immobility behavior in forced-swimming test and immune system enhancing effect of traditional medicine Gamisipjundaebo-tang Pharmacol. Biochem. Behavior. 79 253 - 259
Whicher J. , Spence C. 1987 When is serum albumin worth measuring? Ann. Clin. Biochem. 24 572 - 580
Zhao B.T. , Jeong S.Y. , Hwangbo K. , Moon D.C. , Seo E.K. , Lee D. , Lee J.H. , Min B.S. , Ma E.S. , Son J.K. , Woo M.H 2013 Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD Arch. Pharm. Res 36 1231 - 1237