Cellular ubiquitin pool dynamics and homeostasis
Cellular ubiquitin pool dynamics and homeostasis
BMB Reports. 2014. Sep, 47(9): 475-482
Copyright © 2014, Korean Society for Biochemistry and Molecular Biology
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • Received : June 12, 2014
  • Published : September 30, 2014
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Chul-Woo, Park
Kwon-Yul, Ryu

Ubiquitin (Ub) is a versatile signaling molecule that plays important roles in a variety of cellular processes. Cellular Ub pools, which are composed of free Ub and Ub conjugates, are in dynamic equilibrium inside cells. In particular, increasing evidence suggests that Ub homeostasis, or the maintenance of free Ub above certain threshold levels, is important for cellular function and survival under normal or stress conditions. Accurate determination of various Ub species, including levels of free Ub and specific Ub chain linkages, have become possible in biological specimens as a result of the introduction of the proteomic approach using mass spectrometry. This technology has facilitated research on dynamic properties of cellular Ub pools and has provided tools for in-depth investigation of Ub homeostasis. In this review, we have also discussed the consequences of the disruption of Ub pool dynamics and homeostasis via deletion of polyubiquitin genes or mutations of deubiquitinating enzymes. The common consequence was a reduced availability of free Ub and a significant impact on the function and viability of cells. These observations further indicate that the levels of free Ub are important determinants for cellular protection. [BMB Reports 2014; 47(9): 475-482]
Ubiquitin (Ub) is a highly conserved eukaryotic protein composed of 76 amino acids that induces post-translational modification of target substrates by forming isopeptide linkage via actions of Ub-activating enzymes (E1), Ub-conjugating enzymes (E2), and Ub ligases (E3) (1) . In general, Ub conjugation results in either monoubiquitination or polyubiquitination of target substrates. Monoubiquitination has been well known to regulate receptor endocytosis and histone modification (2) , and polyubiquitination plays diverse functions that are dependent on the type of Ub chain linkages, including degradation of the substrate by the 26S proteasome, DNA repair, and activation of signal transduction pathways (3) . Ub is removed from its target substrates by deubiquitinating enzymes (DUBs) after its functions are completed, and it is recycled back to the free Ub pool (4 , 5) . The free Ub pool is also maintained through the expression of two different classes of ubiquitin genes in mammals: monomeric Ub-ribosomal fusion genes, Uba52 and Uba80 (also known as Rps27 a); and stress-inducible polyubiquitin genes, Ubb and Ubc , composed of 3-4 and 9-10 tandem Ub coding units, respectively (6 - 8) .
Ub inside cells is in dynamic equilibrium between “free” Ub that is readily available for ubiquitination and “conjugated” Ub that is covalently attached to target substrates. Recently, there has been a growing interest into how the free Ub pool is maintained under normal or stress conditions and how disruption of Ub pool dynamics and homeostasis affect the function and viability of cells. In this review, we briefly discuss recent advances in the field of Ub biology, focusing particularly on Ub homoeostasis, dynamic properties of cellular Ub pools, and the consequence of Ub deficiency or depletion in cells and metazoans.
Ub plays diverse functions inside cells, and it is an abundant protein that comprises up to 5% of total proteins (9 , 10) . The substrate proteins of Ub are also abundant; therefore, the maintenance of cellular levels of Ub, or Ub homeostasis, is important for timely ubiquitination of substrate proteins. One way to achieve Ub homeostasis is to regulate monomeric Ub-ribosomal fusion gene and polyubiquitin gene expression ( Fig. 1 ).
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Dynamic properties of cellular Ub pools. Conjugation of protein substrates (S) with Ub by a series of enzymes (E1-E3) and deconjugation by DUBs are in dynamic equilibrium. Ub conjugation results in monoubiquitination or polyubiquitination with different types of chain linkages. Ub conjugates or polyubiquitinated protein substrates with specific chain linkages (e.g., K48-linked chains) are targeted to the 26S proteasome for degradation. During degradation of substrates, Ub chains are removed en bloc by a specific DUB (e.g., POH1) and are further processed to free Ub. The free Ub pool is maintained by de novo Ub synthesis via expression of four different ubiquitin genes and by recycling or removal of Ub from Ub conjugates via actions of various DUBs (e.g., UCH37, USP14). Under certain conditions, Ub itself can also be degraded by the proteasome. See the text for details.
Polyubiquitin genes are known to be upregulated under stress conditions, such as heat shock due to the presence of heat-shock response elements in promoter regions (11 , 12) . Another way to achieve Ub homeostasis is through regulation of DUBs that convert Ub conjugates into their monomeric free forms and increase levels of the free Ub pool ( Fig. 1 ). There are nearly 100 DUBs in humans (13) , and among these, POH1 (or Rpn11 in yeast) - the proteasome lid subunit in the 19S regulatory particle with DUB activity that detaches the polyubiquitin chain en bloc from target substrates during proteasomal degradation (14 , 15) - generates unanchored free Ub chains.
In yeast, Doa4 was shown to be a DUB necessary for recycling Ub from polyubiquitinated proteins targeted to the proteasome for degradation (16) . Doa4 was also shown to cleave unanchored free Ub chains to generate monomeric free Ub (17) . The loss of Doa4 or overexpression of its inhibitor Rfu1 (regulator of free ubiquitin chains 1) resulted in reduced levels of monomeric free Ub and disrupted Ub homeostasis, which rendered cells more susceptible to stress conditions.
The levels of free Ub can also be regulated by degradation of Ub itself ( Fig. 1 ). In fact, Ub is a quite stable protein with a compact and globular structure resulting from significant intramolecular hydrogen bonding, with a short C-terminal tail that may serve as an inefficient target for the proteasome (18) . However, Ub can also be degraded under certain conditions (19) . First, although it may be a slow process, Ub itself can be polyubiquitinated by K48-linked chains and targeted to the proteasome. Second, Ub with a C-terminal tail longer than 20 amino acids with fused or conjugated peptides can be efficiently degraded by the proteasome in a polyubiquitination-independent manner. Third and most importantly, Ub, especially in the basal or proximal part of the polyubiquitin chain, can be degraded with its conjugated substrates.
The degradation of Ub itself is closely related to the activities of DUBs associated with the proteasome since the loss of USP14 (or Ubp6 in yeast) or UCH37 has been shown to trigger degradation of Ub along with its target substrates, leading to depletion of the free Ub pool (20 - 22) . Intriguingly, Ub deficiency induced the upregulation of USP14 in mammals (Ryu KY, unpublished data) and Ubp6 in yeast (23) , thereby sparing Ub from proteasomal degradation and increasing levels of free Ub in an attempt to reach Ub homeostasis.
Cellular Ub pools are composed of Ub conjugated to target substrates via actions of enzymes E1-E3 and of unanchored free Ub synthesized de novo or recycled by DUBs. The dynamic properties of cellular Ub pools were first confirmed by microinjection of radiolabeled Ub into HeLa cells. Upon heat shock, levels of free Ub and ubiquitinated histones decreased and levels of high MW Ub conjugates increased (24) . The increase of high MW Ub conjugates did not require synthesis of Ub de novo , but was rather due to redistribution of the existing free Ub pool. In fact, upon removal of heat shock, the levels of free Ub, ubiquitinated histones, and high MW Ub conjugates returned to those of normal conditions. Furthermore, when proteotoxic stress was induced by the presence of a proteasome inhibitor in cell lines stably expressing GFP-tagged Ub, nuclear Ub conjugated to histone 2A (uH2A) was redistributed to cytoplasmic Ub conjugates, demonstrating the dynamic nature of Ub pools (25) .
Various strategies have been employed to monitor the status of Ub in cells since cellular Ub pools are known to be dynamic rather than static. Immunoblot analysis via anti-Ub antibodies is a traditional method that detects free Ub and Ub conjugates. However, due to the nature of antibodies that prefer, but do not bind exclusively to, specific forms of Ub, an accurate determination of total Ub levels or of the ratio between free Ub and Ub conjugates has not been easy to achieve. To overcome this problem, a simple and reliable method was developed that quantifies the total Ub levels using indirect competitive ELISA (Ub-ELISA) after converting all Ub conjugates to monomeric free Ub using the Ub-specific protease Usp2-cc (10) . However, Ub-ELISA has a limitation in that only the levels of total Ub can be determined accurately, whereas the mass spectrometry-based approach enables Ub researchers to quantify diverse cellular Ub species, as described below.
A proteomic approach was implemented to study protein ubiquitination at large scale. Trypsin digestion of ubiquitinated proteins or ubiquitinome yielded a signature peptide containing K-ε-GG since isopeptide-linked or modified Lys cannot be cleaved by trypsin (26) . This Gly-Gly modification on Lys of the fragmented peptide can be detected via tandem mass spectrometry (MS/MS) due to a mass shift of 114 Da. Using this technique, more than 1,000 ubiquitinated proteins were identified in cell lysates from yeast expressing His-tagged Ub. Furthermore, 110 ubiquitination sites from 72 different proteins were identified (26) . In particular, Ub itself was found to harbor 7 ubiquitination sites, suggesting a diverse nature of polyubiquitin chain assembly.
Since then, absolute quantification (AQUA), another proteomic strategy, has been developed to quantify the amount of polyubiquitin chains in biological specimens using liquid chromatography-tandem mass spectrometry (LC-MS/MS) (27 , 28) . In this strategy, ubiquitinated proteins are enriched, and then a known amount of stable isotope-labeled synthetic peptide standard is added, and the amount of branched peptide fragments generated by trypsin digestion is compared to that of the peptide standard. Through this method, the exact amount of specific polyubiquitin chains can be determined ( Fig. 2 ). This strategy also facilitated the investigation of the dynamic nature of Ub pools in neurodegenerative diseases (29) . Recently, antibodies that recognize the K-ε-GG peptide have been developed (30) , facilitating pull-down and enriching K-ε-GG peptides from ubiquitinated proteins in cell lysates, and the dynamic changes in Ub pools and ubiquitination of proteins were observed by enriching K-ε-GG peptides in stable isotope labeling with amino acids in cell culture (SILAC)-encoded cells under proteasome or DUB inhibition (31) .
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Mass spectrometry-based approach to quantify cellular Ub pools. (Left) The amount of protein substrates (S) with specific Ub chains can be determined accurately via AQUA. After enriching ubiquitinated substrates, branched signature peptide fragments are generated by trypsin digestion since trypsin cannot cleave modified Lys. A known amount of stable isotope-labeled synthetic peptide standard (AQUA standard) is added into samples during trypsin digestion. Then, by comparing ion intensities, the signature peptide fragments are quantified after LC-MS analysis. For simplicity, only the K48-linked chains are shown. (Right) The amount of free Ub, total Ub, and protein substrates (S) with Ub chains can be quantified via Ub-PSAQ. Samples are mixed with a known amount of stable isotope-labeled protein recovery standard. Half of the sample is affinity-captured with a BUZ domain that specifically binds free Ub, followed by a UBA domain that specifically binds polyUb chains. The other half of the sample is deubiquitinated by treatment with Usp2-cc and is affinity-captured with the BUZ domain to quantify the total amount of Ub. After elution, the AQUA standard is added during trypsin digestion, and LC-MS analysis is carried out. Ion intensities of endogenous peptide fragments are compared to both the AQUA standard (for quantification of peptide fragments in the eluate) and protein standard (for determination of recovery from the affinity column), and the peptide fragments are quantified.
In addition to the quantification of polyubiquitin chains, protein standard absolute quantification (Ub-PSAQ) - a method for accurate measurement of diverse cellular Ub pools - was also developed (32) . In this method, free Ub species are affinity- captured with Ub-binding Zn finger (BUZ) domain, and then polyubiquitin chains are affinity-captured with Ub-associated (UBA) domain ( Fig. 2 ). To determine the amount of total Ub, Usp2-cc is treated to convert all forms of Ub conjugates to monomeric free Ub, and free Ub is then captured with the BUZ domain. After elution and trypsin digestion, peptide fragments are analyzed via liquid chromatography-electrospray ionization time-of-flight mass spectrometry (LC-ESI TOF MS) and are quantified by comparing to stable isotope-labeled peptides recovered from free Ub and Ub conjugate standards that are spiked into the samples before affinity capture. The concentration of monoubiquitinated species is determined by subtracting the amount of free Ub and polyubiquitin chains from that of the total Ub.
According to the results of Ub-PSAQ, more than 20% of total Ub pools were composed of free Ub, and more than 60% were composed of monoubiquitinated proteins in HEK293 cells and mouse embryonic fibroblasts (MEFs), suggesting that proteins with the polyubiquitin chain are less than 20% of the total Ub pools. Surprisingly, Ub pool distribution was different for brain tissues when compared to HEK293 cells and MEFs. In mouse brains, 60% of the total Ub pools were free Ub and proteins with polyubiquitin chains were only 5% of the composition. In the human frontal cortex, free Ub content was even higher, up to 80% (32) . The immunoblot analysis was consistent with the results of Ub-PSAQ data and also revealed that levels of free Ub in mouse brain and cultured neurons were much higher than those in HEK293 cells and MEFs (Ryu KY, unpublished data). Therefore, cellular contents of different Ub species may vary depending on the type of cells, and high levels of free Ub in neurons suggest an important role of Ub for neuronal function and survival.
Although polyubiquitinated proteins may not be abundant in any cell types under normal conditions, they constitute a significant portion of total Ub pools under stress conditions, and the effect of polyubiquitination on target proteins are different depending on the type of Ub chain linkages. To investigate the role of Ub chain linkages, polyubiquitin chain-specific antibodies were developed which recognize specific types of chains such as K48- or K63-linked (33) . These facilitated research investigating mechanisms of polyubiquitin chain assembly ( Fig. 3 ).
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Various types of ubiquitination and the specific role of Ub chain linkages. Ub is composed of 76 amino acids and forms isopeptide linkages between the carboxyl group of Gly76 residue and ε-amino group of Lys residue in substrate or in another Ub molecule. The substrate can be monoubiquitinated, multi-monoubiquitinated, or polyubiquitinated with different types of chain linkages. The 1st Met and seven Lys residues in Ub (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63) can all form specific chain linkages with distinct conformations and can exert specific (or unknown) functions. Although it is rare, heterogeneous and branched/forked chain linkages are also possible.
However, the dynamic nature of chain elongation and shortening, the formation of branched or forked Ub chains, and even the formation of heterogeneous chains that contain a mixture of different types of linkages have hampered Ub researchers from obtaining a complete understanding of the link between chain formations and their biological consequences (34 - 36) . Nevertheless, K48- or K63-linked polyubiquitin chains are well known for their respective roles in proteasomal degradation or in other non-proteasomal cellular processes.
The roles of other unconventional polyubiquitin chains (K6, K11, K27, K29, or K33) have also been investigated. For example, K48-linked polyubiquitin chains as well as an accumulation of K11-linked polyubiquitin chains were observed in yeast upon inhibition of the proteasome, and Ubc6 was identified via quantitative proteomic analysis using LC-MS/MS as a target substrate for polyubiquitination with K11 linkages. Interestingly, Ubc6 also served as an E2 enzyme in conjunction with an E3 ligase Doa10 to form K11-linked polyubiquitin chains on misfolded ER proteins and for targeting them to the proteasome for degradation. This is called the endoplasmic reticulum-associated degradation (ERAD) pathway and K11R mutant, which cannot form K11 linkages, were shown to be more sensitive to ER stress (37) . A quadruple mutation (K6R, K11R, K27R, and K63R) in yeast increased the levels of K33-linked polyubiquitin chains by more than 20-fold, suggesting the redundant role of different chain linkages. However, this was not sufficient to fully protect cells from reduced proliferation and viability in quadruple mutants (37) . Therefore, although all non-K63 linkages may serve as a signal to target proteins for proteasomal degradation, specific target proteins may need to be polyubiquitinated with different chain linkages.
Alterations in Ub pools and on its phenotypic consequences were investigated through the deletion of polyubiquitin genes in yeast and in mammals. The deletion of the stress-inducible polyubiquitin gene UBI4 did not affect the viability of yeast under normal vegetative growth conditions but did cause the yeast to exhibit an increased sensitivity against heat shock, starvation, and other stress conditions (38) .
In addition, the depletion of free Ub via cycloheximide treatment caused toxicity with reduced viability in yeast (39) . Mutations in the proteasome that spare Ub from degradation or overexpression of Ub in yeast conferred resistance against cycloheximide-induced toxicity. Although it was expected for the steady-state levels of many proteins to be affected by the inhibition of de novo protein synthesis by cycloheximide treatment, it is quite surprising that perturbations in Ub homeostasis are most critical for the viability of yeast. These results indicate that maintaining adequate levels of free Ub, which could be rate limiting, is essential for cellular survival.
In mice, there are two polyubiquitin genes, Ubb and Ubc , and they were shown to be required even under normal growth conditions. Homozygous deletion of Ubc caused mid-gestation embryonic lethality due to defective proliferation of hepatocytes and bipotent fetal liver epithelial progenitor cells (40 , 41) . This result was surprising because, based on previous studies on yeast, it had been believed that the polyubiquitin gene is required under stress conditions, but is dispensable under normal growth conditions (38) . On the other hand, homozygous deletion of Ubb caused infertility and adult-onset hypothalamic neurodegeneration with metabolic and sleep abnormalities (42 - 44) . These phenotypes in Ubb knockout mice are closely associated with the expression profiles of Ubb since Ubb is highly expressed in germ cells and in hypothalamic neurons. Therefore, the disruption of Ubb reduced the levels of Ub most dramatically for these specific cell types. Intriguingly, Ubb was also highly expressed in the locus coeruleus region of the brain. However, locus coeruleus neurons were well-protected, even when Ubb was disrupted (45) . Although total Ub levels were reduced by 30% in both the hypothalamus and in the locus coeruleus of Ubb knockout mice, free Ub levels were reduced by 40% only in the hypothalamus of Ubb knockout mice but not in the locus coeruleus. These observations suggest that the levels of free Ub in neurons or in neighboring cells are important for neuronal function and survival.
In Ubc knockout MEFs, the total Ub levels were reduced by 40%, reflecting the significance of Ubc contribution toward total Ub levels in MEFs. The deletion of Ubc in MEFs caused reduced proliferation, premature senescence, and delayed entry into mitosis (40) . Therefore, the relative contributions of Ubb and Ubc toward the total Ub levels, resulting from their transcriptional activities, are different depending on the types of cells or tissues. Due to different tissue spectrum of Ubb and Ubc expression, the deletion of one polyubiquitin gene may not necessarily be fully compensated for by the upregulation of another polyubiquitin gene. As cell cycle progression can be regulated by the expression levels of polyubiquitin genes and increased levels of Ub are closely associated with the proliferation of cancer cells, knockdown of Ubb has been shown to induce apoptosis in cancer cells (46) . Interestingly, knockdown of Ubb dramatically reduced the levels of free Ub, but not of Ub conjugates. Therefore, levels of free Ub are closely associated with cell viability.
Ub pools can also be regulated by DUBs. DUBs are classified into five families based on their domains, and these are known as Ub C-terminal hydrolase (UCH), Ub-specific protease (USP), ovarian tumor (OTU), Josephin (MJD), and JAB1/MPN/Mov34 metalloenzyme (JAMM) (13) . Among these, Ub C-terminal hydrolase L1 (UCH-L1) is one of the most abundant proteins in the brain, comprising up to 5% of the total of soluble proteins (47) . Mutations in Uchl1 are closely linked to the susceptibility of humans to Parkinson’s disease (PD) (48) . In mice, deletion or loss-of-function mutations in Uchl1 caused gracile axonal dystrophy ( gad ), which is an autosomal recessive disorder with sensory and motor ataxia (49) .
Due to dysfunction of UCH-L1 in gad mice, the free Ub pool was disturbed, leading to an accumulation of Ub-positive abnormal protein deposits in the nervous system. The importance of UCH-L1 has also been implicated in the pathogenesis of Alzheimer’s disease (AD) (50) . Soluble UCH-L1 protein levels, but not mRNA levels, were reduced in AD model APP/PS1 mice, and the exogenous expression of UCH-L1 improved synaptic function and contextual learning in APP/PS1 mice. In gad mice, the levels of free Ub in the brain were significantly reduced, but levels of Ub conjugates or ubiquitin gene expression were not affected (51) . Therefore, UCH-L1 exerts an essential role in maintaining free Ub levels.
Mutations in Usp14 , which encodes Ub-specific protease, are also known to cause neurological disorders, including synaptic dysfunction (52) . Since the ataxia (ax) gene encodes USP14, the expression of intact USP14 protein was not observed in axJ mice. Free Ub, but not Ub conjugate, levels were reduced in the brain of axJ mice, but neuron-specific expression of the Usp14 transgene was sufficient to restore free Ub levels in the spinal cord and motor neuron axons and could rescue ataxia phenotypes with defective neuromuscular junctions in axJ mice (53 , 54) . In axJ mice, the levels of free Ub were reduced by 30%, not only in the brain, but also in other tissues such as the liver, heart, and spleen (20) . In synaptosomal fractions of axJ mice, however, the levels of free Ub were further reduced by 60%, with a concomitant reduction in Ub conjugates by 40%, suggesting there to be a significant impairment in Ub homeostasis at the synapse (53) . Since free Ub needs to be transported along the axon down to the synapse, and this axonal transport of free Ub is a quite slow process (55) , the levels of synaptic Ub could be further reduced due to the dysfunction of USP14. Synaptic dysfunction in axJ mice was also rescued by neuron-specific expression of ubiquitin transgene, which restored levels of free Ub (56) .
Although mutations in two different deubiquitinating enzymes UCH-L1 and USP14 lead to neurological abnormalities in mice with reduced levels of free Ub, their effects on the viability of mice and on the onset of symptoms were much more severe, and the altered synaptic transmission in the hippocampus was only observed when Usp14 was mutated or lost (57) . Since loss-of-function mutations in Usp14 upregulate the expression of proteasomal subunits, and mutations in Uchl1 upregulate the expression of lysosomal proteases, each DUB may be involved in the regulation of Ub pools in different subcellular compartments.
As briefly summarized in this review, numerous research has been carried out during the past three decades to investigate cellular Ub pools, revealing the importance of polyubiquitin genes and of DUBs in maintaining threshold levels of the free Ub pool. Since cells may not distinguish polyubiquitin gene-encoded Ub from monoubiquitin gene-encoded Ub after the deubiquitination of Ub adduct or Ub-ribosomal fusion proteins, the outcome of polyubiquitin gene deletion is very simple in that the availability of cellular Ub is reduced. It is interesting to note that the reduced availability of Ub is sufficient to cause adverse effects on cellular function and survival. In particular, a reduced viability of cells due to disruption of Ub homeostasis accompanied a concomitant reduction of free Ub levels. Although it has yet to be determined whether or not disruption of Ub homeostasis is the cause or the effect of neuropathological abnormalities observed in polyubiquitin gene-deficient models, it is quite reasonable to speculate that levels of free Ub are an important determinant of cellular vulnerability. Furthermore, as a result of the dynamic properties of Ub pools, free Ub can be converted to Ub conjugates, which sometimes form polyubiquitinated protein deposits under stress conditions. Abnormal protein deposits tend to be clustered with reduced solubility. Therefore, sequestration of free Ub into insoluble fractions can also reduce the availability of free Ub and can disrupt Ub homeostasis, potentially affecting various cellular processes that require protein ubiquitination or degradation. The development of a strategy to maintain Ub homeostasis (above the threshold levels) under stress conditions is required to protect cells from various extracellular insults.
This work was supported by the 2012 Research Fund of the University of Seoul to K. Y. R.
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