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Mouse models of polycystic kidney disease induced by defects of ciliary proteins
Mouse models of polycystic kidney disease induced by defects of ciliary proteins
BMB Reports. 2013. Feb, 46(2): 73-79
Copyright © 2013, Korean Society for Biochemistry and Molecular Biology
This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • Received : January 24, 2013
  • Published : February 27, 2013
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About the Authors
Je Yeong Ko
Jong Hoon Park
parkjh@sookmyung.ac.kr

Abstract
Polycystic kidney disease (PKD) is a common hereditary disorder which is characterized by fluid-filled cysts in the kidney. Mutation in either PKD1 , encoding polycystin-1 (PC1), or PKD2 , encoding polycystin-2 (PC2), are causative genes of PKD. Recent studies indicate that renal cilia, known as mechanosensors, detecting flow stimulation through renal tubules, have a critical function in maintaining homeostasis of renal epithelial cells. Because most proteins related to PKD are localized to renal cilia or have a function in ciliogenesis. PC1/PC2 heterodimer is localized to the cilia, playing a role in calcium channels. Also, disruptions of ciliary proteins, except for PC1 and PC2, could be involved in the induction of polycystic kidney disease. Based on these findings, various PKD mice models were produced to understand the roles of primary cilia defects in renal cyst formation. In this review, we will describe the general role of cilia in renal epithelial cells, and the relationship between ciliary defects and PKD. We also discuss mouse models of PKD related to ciliary defects based on recent studies. [BMB Reports 2013; 46(2): 73-79]
Keywords
INTRODUCTION
Polycystic kidney disease (PKD) is a common genetic disorder and is manifested by defects in renal ithelial cells, resulting in an increase of cell proliferation and cyst formation (1) . PKD is a systemic disorder which is characterized by cysts in thekidney, liver and pancreas (2) .
ADPKD is caused by mutations of either PKD1 (85%) or PKD2 (15%) genes, which encode polycystin-1 (PC1) or polycystin-2 (PC2) respectively (3) . PC1 is a large integral membrane protein, which includes a long N-terminal extracellular domain, a short C-terminal intracellular domain, and eleven transmembrane domains, and acts as a G-protein-coupled receptor (4 - 6) . PC1 is localized to primary cilia, lateral domain of the plasma membrane (7) , and regions of cell-matrix interaction (8) . The C-terminal, cytoplasmic tail, of PC1 is cleaved to generate products, which interact with transcription factors. PC2 is a membrane protein with six transmembrane domains, and is considered to be a cation channel with specificity for calcium (9 , 10) . This protein is localized to not only primary cilia (11) , but also to subcellular compartments, including the plasma membrane (12) and endoplasmic reticulum. PC1 and PC2 interact via their C-terminus (13) to form transmembrane receptor-ion channel complex in the primary cilia of renal epithelial cells (1 , 14) . This protein complex regulates intracellular calcium levels through its channel, which is stimulated by fluid flow in the renal tubule, and affects calcium-related pathways. Recent studies have shown that dysfunctions of PC1 and PC2 result in decreased intracellular calcium in renal epithelial cells of PKD (15) . Some aberrant signaling pathways have been identified in PKD. Among these, the signaling pathway related to calcium, cyclic AMP (cAMP) and mammalian target of rapamycin (mTOR) is well-known as a major signaling in PKD (16 - 19) . In addition to these pathways, dysregulation of cell cycle, JAK-STAT pathway, canonical and non-canonical Wnt pathway are observed in PKD.
Recent studies suggest that defects of primary cilia are also related to the formation of renal cystic kidneys (20 , 21) . The cilium acts as a mechanosensor in kidney tubules, in regulating homeostasis of renal epithelial cells. Many signaling components are localized to the membrane of renal primary cilia. Accumulating results show that proteins related to PKD are localized to the primary cilia, and disruptions of ciliary proteins, including intraflagellar transport (IFT), induce PKD (3) . These findings indicate that defects of cilia act as a driving force to induce PKD (22) .
Consistent with these recent findings, malfunctions of primary cilia, induced by mutations of ciliary proteins, is correlated with PKD. Therefore, it is important to identify the cyst formation mechanism associated with ciliary malfunctions using in vivo studies, to develop therapeutic targets for PKD. Here, we will focus on recent advances in understanding the ciliary roles in polycystic kidney and PKD mouse models accompanied by cilia defects.
PRIMARY CILIA AND IFT
The cilium is commonly divided into two groups, motile and non-motile cilia. Motile cilia that have a 9+2 microtubule configuration (3) are mainly located on epithelial cells, and promote the movement of substances. On the other hand, non-motile cilia, known as primary cilia, have a 9+0 microtubule arrangement. Primary cilia projected from the cell membrane act as mechanosensor to sense mechanical forces, growth factors and chemical compounds from extracellular environments (23) . Primary cilia have important roles for regulating intracellular signaling, such as Hedgehog (Hh), Wnt, platelet- derived growth factor receptor (PDGFR) α, and mechanosignaling related to PKD1/2 complex (24) .
Regulation of ciliogenesis is critical to maintain functional cilia. The key factor related to ciliogenesis is Intraflagellar Transport (IFT), which is a bi-directional movement multi-protein complex (25) . IFT particles are divided into two complexes, A and B. The complex A, known as retrograde transport, moves from ciliary tip to base with dynein, while the complex B, known as anterograde transport, moves from base to ciliary tip with kinesin-2 (26) . IFT particles are regarded as vehicles for transporting cargos to regulate cilia assembly, maintenance, and function (27) . In addition, these particles have a function in carrying cilia membrane proteins. Consistent with these roles for cilia, cilia defects or mutations of IFT genes are related to human disease, such as PKD, left-right asymmetry defects, cancer, and Bardet-Biedl syndrome (BBS) (24 , 28) .
RELATIONSHIP BETWEEN CILIA DEFECTS AND POLYCYSTIC KIDNEY DISEASE
In renal epithelial cells, primary cilia protrude into lumen of the renal tubules to sense fluid flow (29) . Polycystin-1 (PC1) and polycystin-2 (PC2) are localized to the membrane of cilium, and form a PC1/PC2 complex as a calcium channel. PC1 acts as mechanosensor to detect the bending of cilium induced by flow stimulation that transmits extracellular signals to PC2, which, in turn, induces Ca 2+ influx to cytosol (11 , 30) , hence this complex and primary cilia in renal cells regulates intracellular signaling associated with calcium concentration such as the Raf/MEK/ERK pathway, which are increased in PKD (31) . Moreover, various signaling molecules related to Hh, PDGFRα, and calcium signaling are localized to the cilia membrane (32) . Therefore, defects of cilia or ciliary proteins are sufficient to induce pathological disorders.
Among various diseases, PKD is one which is related to cilia defects. Many proteins whose functions are disrupted in PKD are localized to cilium membrane or to the ciliary basal body (24 , 33) . Also, disruption of cilia proteins are involved in calcium signaling related to PKD, implicating a relationship between ciliary signaling and cyst formation (34) . Consistent with this, the intracellular calcium level is decreased in PKD epithelial cells compared to normal renal epithelial cells (35) . Recent studies have demonstrated that malfunction of renal cilia induces intracellular cAMP, leading to an increase of renal cell proliferation through the Raf/MEK/ERK pathway, known as a hallmark of cystic kidney (36 , 37) . Therefore, to elucidate the functions of ciliary genes in vivo is important to identify the disease inducing mechanisms and therapeutic targets of PKD.
MOUSE MODELS OF POLYCYSTIC KIDNEY INDUCED BY MUTATIONS OF CILIARY PROTEINS
- Kidney specific inactivation of Pkd1
As we described above, PC1 localized to primary cilia is a major causative gene for the development of polycystic kidneys. In the case of complete knockout of Pkd1 in the mouse, a complex embryonic lethality is observed: defects in formation of the kidney, pancreas, heart and capillary blood vessels (38 - 40) . However, Pkd1flox/-:Ksp-Cre mice generated by deletion of exons 2-4 by Ksp-Cre are born, but consistently develop cystic kidneys (14) . Consistent with this, components related to the MAPK/ERK pathway, blood urea nitrogen (BUN), and cell proliferation, are dramatically increased in the cystic kidney of this mouse (14) .
- Pkd2ws25/-mice
The WS25 allele is generated by the integration of an exon 1 disrupted by introduction of a neor cassette into intron 1 of Pkd2 , without replacement of the wild-type exon 1 (41 , 42) . This mutation causes an unstable allele, which increases the somatic mutation rate of Pkd2 (42 , 43) . The knockout of Pkd2 is embryonically lethal between embryonic day 13.5 and parturition, whereas the Pkd2 ws25/- mouse is born (44) . The Pkd2 ws25/- mouse is an animal model of ADPKD with hepatic cysts and cardiac defects, and exhibits renal failure (44) . Studies using this mouse model have shown that renal cilia in the cystic kidney of Pkd2 ws25/- mouse have normal phenotype (45) , but cilia of cystic cholangiocytes are short and malformed (46) .
- Oak ridge polycystic kidney mice
The oak ridge polycystic kidney (ORPK) mouse resembles human ARPKD with respect to cystic kidneys and hepatic disease (47 , 48) . This mouse is the first model showing a relationship between polycystic kidney and ciliary malfunction (48 , 49) . The orpk allele was driven by insertion mutation on the intron near the 3’end of the gene, partially disrupting the function and expression of Ift88 ( Tg737 , polaris) (50) . Increased levels of PC2 in primary cilia is observed in the kidneys of ORPK mice (49) . The Tg737 orpk mutant mice have polycystic kidneys with shortened cilia on renal epithelial cells (47 , 48) . In addition to renal disorder, hepatic and pancreatic defects, skeletal patterning abnormalities and hydrocephalus are observed in the ORPK mouse (51 - 53) .
- Deletion of Ift20 in the mouse kidney
Inactivation of Ift20 in the mouse kidney using Cre-loxP system promotes postnatal cystic kidneys lacking cilia (54) . The conditional allele of Ift20 was generated by targeting exon 2 and 3 (54) . Deletion of Ift20 in kidneys collecting duct cells induces mis-orientation of the mitotic spindle and increases of canonical Wnt signaling, leading to increased renal cell proliferation (54) . In addition to this in vivo study, there are some evidences showing that Ift20 is a candidate gene related to PKD. Ift20 is associated with Golgi complex to regulate cilia assembly and is involved in the localization of PC2 to cilia (55) .
- Mice with a conditional allele for Ift140
Deletion of Ift140 , subunit of IFT complex A, in renal collecting duct cells leads to renal cyst formation with disruption of the cilia assembly, but this mutation does not have a function on mitotic spindle orientation (56) . Several genes which are hallmarks of polycystic kidney and are associated with the canonical Wnt pathway, Hedgehog, Hippo and fibrosis were significantly increased in Ift140 -deleted kidneys (56) .
- Kif3α-targeted mouse
Constitutive knockout of Kif3α , a subunit of kinesin-II that is critical for ciliogenesis, results in an absence of cilia in the embryonic node, situs inversus, and abnormalities of the neural tube, pericardium and smites (57 , 58) . This constitutive Kif3α knockout mouse shows embryonic lethality before renal organogenesis (59) . In contrast, in the case of specific targeting of Kif3α in renal tubular epithelial cells, viable offspring were born and developed cystic kidneys (59) . These cystic lining epithelial cells have no cilia due to Kif3α inactivation, and show increased proliferation, apoptosis, expression of β-catenin, c-Myc, and decreased p21 cip1 (59) .
- Congenital polycystic kidney mouse
The Cystin encoded by Cys1 is a 145-amino acid cilium-associated protein (60) . The congenital polycystic kidney ( cpk ) mice have mutations of Cystin localized to the primary cilia (61 - 64) . This mutant develops renal cysts and biliary dysgenesis and is considered as ARPKD (61) . The kidneys of cpk/cpk mice have multiple cellular and extracellular abnormalities (63) , including increased expression of proto-oncogenes, such as c-myc, c-fos (65 , 66) , elevated expression of growth factors (67) , abnormal expression of genes related to cell adhesion (68) , and overexpression of matrix metalloproteinases (MMPs) (69) . The renal cystic epithelial cells of cpk mice have different length and morphology of cilia (70) .
- Inversin-targeted mice
The Inversin protein localizes to the primary cilia, cell junctions and nucleus, and acts as a regulator of β-catenin activity and canonical Wnt pathway (71) . The inversion of embryonic turning ( inv ) mutation is generated by random insertion of tyrosinase minigene in the OVE210 transgenic line (72 , 73) . This random insertion disrupts Inversin encoded by Invs , which localizes to primary apical cilia (74) . Phillips et al . (75) have demonstrated that normal looking monocilia were observed in cystic kidneys of inv/inv mice. However, the inv mutant shows a reversal of left-right asymmetry, and renal cyst known as diseases induced by ciliary defects (76) .
- Bbs2-deficient mice
The proteins encoded by Bardet-Biedl syndrome (BBS) genes, except for BBS6, are localized to ciliated organisms (77 , 78) . BBS2 is one of the proteins that form the complex BBSome, which is localized to the membrane of the cilium and is required for ciliogenesis. Several evidences suggest that BBS4 and BBS6 are expressed in basal bodies of primary cilia (79 , 80) . The ciliogenic function of the BBS complex is related to the Rab8 GDP/GTP exchange factor, which localizes to basal body and contacts the BBSome (81) .
Bbs2-deficient mice are generated by deletion of exons 5-14, and show the phenotype of cystic kidneys (82) . Interestingly, renal cilia are observed in Bbs2-deficient mice, but their shape is abnormal compared to cilia of wild-type kidneys (82) . In addition to polycystic kidney, this mutant mouse develops retinal degeneration, obesity and olfactory dysfunction (82) .
- Juvenile cystic kidney mouse
The jck allele has a missense mutation in the NIMA (never in mitosis A)-related kinase 8 encoded by Nek8 (83) . NIMA is a serine/threonine kinase in Aspergillus nidulans , and has a function in regulating mitosis (84) . Published results suggest that Nek8 is overexpressed in human breast cancers, indicating that Nek8 has a role in cell proliferation (84 , 85) .
In wild-type kidney, Nek8 is found in the proximal region of renal cilia, whereas kidneys of the jck mutant show that Nek8 is localized to the entire length of renal cilia (84) . The kidneys of jck mice have cysts in multiple nephron segments like ADPKD, and increased cAMP levels, which promote renal cell proliferation and fluid secretion (86) . Also, the lengthened cilia with overexpression of polycystins are observed in kidneys of jck mice (86) .
CONCLUSION
The role of the renal primary cilia is under the spotlight, because the disruption of ciliary structures and proteins is associated with polycystic kidney. To determine the mechanism between defects of renal cilia and polycystic kidneys, various cystic kidney mouse models have been generated. Based on many studies, mutation of some proteins localized to primary cilia, such as IFT, leads to cyst formation accompanied by ciliary defects. This mechanism of cyst formation seems to occur via increased renal cell proliferation. Consistent with this, mutations of some proteins localized to ciliary membrane, such as PC1 and PC2, result in the development of cystic kidneys. However, these ciliary membrane proteins, non-IFT family proteins, which act as receptor and signaling molecules, seem to have no effect on the regulation of ciliogenesis. Instead of functioning in ciliogenesis, these proteins are localized to ciliary membranes to regulate the cilia function as mechanosensors rather than regulating ciliogenesis. Taken together, dysfunctions of proteins associated with renal primary cilia have an effect on cyst formation of the kidneys ( Fig. 1 ). To identify the exact mechanism of cyst formation induced by ciliary defects, further in vivo studies are needed. It is important to reveal the mechanism of cystic kidney development, because a successful treatment for PKD has not yet been developed. Therefore, we expect that these mechanism studies might be used for the selection of novel therapeutic targets of PKD.
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Lager Image
Localization of ciliary proteins related to polycystic kidney. Many proteins localized to renal cilia are associated with PKD. Disruptions of these proteins induce cystic kidneys through various signaling pathway.
Acknowledgements
This Research was supported by the Sookmyung Women's University Research Grants 2010.
References
Fedeles S. , Gallagher A. R. (2012) Cell polarity and cystic kidney disease. Pediatr. Nephrol. 2012 Nov 16 [Epub ahead of print] PubMed PMID: 23161205
Wilson, P. D. , Goilav, B. Cystic disease of the kidney. Ann. Rev. Pathol. 2 341 - 368    DOI : 10.1146/annurev.pathol.2.010506.091850
Nauli, S. M. , Zhou, J. (2004) Polycystins and mechanosensation in renal and nodal cilia. Bioessays 26 844 - 856    DOI : 10.1002/bies.20069
Nims N. , Vassmer D. , Maser R. L. (2003) Transmembrane domain analysis of polycystin-1, the product of the polycystic kidney disease-1 (PKD1) gene: evidence for 11 membrane- spanning domains. Biochemistry 42 13035 - 13048    DOI : 10.1021/bi035074c
Li X. (2011) Phosphorylation, protein kinases and ADPKD. Biochim. Biophys. Acta 1812 1219 - 1224    DOI : 10.1016/j.bbadis.2011.03.001
Delmas P. , Nomura H , Li X. , Lakkis M. , Luo Y. , Segal Y. , Fernandez-Fernandez J. M. , Harris P. , Frischauf A. M. , Brown D. A. , Zhou J. (2002) Constitutive activation of G-proteins by polycystin-1 is antagonized by polycystin-2. J. Biol. Chem. 277 11276 - 11283    DOI : 10.1074/jbc.M110483200
Chapin H. C. , Caplan M. J. (2010) The cell biology of polycystic kidney disease J. Cell Biol. 191 701 - 710    DOI : 10.1083/jcb.201006173
Wilson P. D. , Geng L. , Li X. , Burrow C. R. (1999) The PKD1 gene product, "polycystin-1," is a tyrosine-phosphorylated protein that colocalizes with alpha2beta1- integrin in focal clusters in adherent renal epithelia. Lab. Invest. 79 1311 - 1323
Ibraghimov-Beskrovnaya O , Bukanov N. (2008) Polycystic kidney diseases: from molecular discoveries to targeted therapeutic strategies. Cell. Mol. Life Sci. 65 605 - 619    DOI : 10.1007/s00018-007-7362-x
Gonzalez-Perrett S. , Kim K. , Ibarra C. , Damiano A. E. , Zotta E. , Batelli M. , Harris P. C. , Reisin I. L. , Arnaout M. A. , Cantiello H. F. (2001) Polycystin-2, the protein mutated in autosomal dominant polycystic kidney disease (ADPKD), is a Ca2+-permeable nonselective cation channel Proc. Natl. Acad. Sci. U.S.A. 98 1182 - 1187    DOI : 10.1073/pnas.98.3.1182
Nauli S. M. , Alenghat F. J. , Luo Y. , Williams E. , Vassilev P. , Li X. , Elia A. E. , Lu W. , Brown E. M. , Quinn S. J. , Ingber D. E. , Zho J. (2003) Polycystins 1 and 2 mediate mechanosensation in the primary cilium of kidney cells. Nat. Genet. 33 129 - 137    DOI : 10.1038/ng1076
Hanaoka K. , Qian F. , Boletta A. , Bhuni A. K. , Pionte K. , Tsiokas L. , Sukhatme V. P. , Guggino W. B. , Germino G. G. (2000) Co-assembly of polycystin-1 and -2 produces unique cation-permeable currents. Nature 408 990 - 994    DOI : 10.1038/35050128
Qian F. , Germino F. J. , Cai Y. , Zhang X. , Somlo S. , Germino G. G. (1997) PKD1 interacts with PKD2 through a probable coiled-coil domain. Nat. Genet. 16 179 - 183    DOI : 10.1038/ng0697-179
Shibazaki S. , Yu Z. , Nishio S. , Tian X. , Thomson R. B. , Mitobe M. , Louvi A. , Velazquez H. , Ishibe S. , Cantley L. G. , Igarashi P. , Somlo S. (2008) Cyst formation and activation of the extracellular regulated kinase pathway after kidney specific inactivation of Pkd1. Hum. Mol. Genet. 17 1505 - 1516    DOI : 10.1093/hmg/ddn039
Kip S. N. , Hunter L. W. , Ren Q. , Harris P. C. , Somlo S. , Torres V. E. , Sieck G. C. , Qian Q. (2005) [Ca2+]i reduction increases cellular proliferation and apoptosis in vascular smooth muscle cells: relevance to the ADPKD phenotype. Circ. Res. 96 873 - 880    DOI : 10.1161/01.RES.0000163278.68142.8a
Tao Y. , Kim J. , Faubel S. , Wu J. C. , Falk S. A. , Schrier R. W. , Edelstein C. L. (2005) Caspase inhibition reduces tubular apoptosis and proliferation and slows disease progression in polycystic kidney disease. Proc. Natl. Acad. Sci. U.S.A. 102 6954 - 6959    DOI : 10.1073/pnas.0408518102
Torres V. E. , Bankir L. , Grantham J. J. (2009) A case for water in the treatment of polycystic kidney disease. Clin. J. Am. Soc. Nephrol. 4 1140 - 1150    DOI : 10.2215/CJN.00790209
Chang M. Y. , Ong A. C. (2012) Mechanism-based therapeutics for autosomal dominant polycystic kidney disease: recent progress and future prospects. Nephron. Clin. Pract. 120 25 - 35    DOI : 10.1159/000334166
Park E. Y. , Woo Y. M. , Park J. H. (2011) Polycystic kidney disease and therapeutic approaches. BMB Rep. 44 359 - 368    DOI : 10.5483/BMBRep.2011.44.6.359
Ibraghimov-Beskrovnaya O. (2007) Targeting dysregulated cell cycle and apoptosis for polycystic kidney disease therapy. Cell Cycle 6 776 - 779    DOI : 10.4161/cc.6.7.4047
Pan J. , Wang Q. , Snell W. J. (2005) Cilium-generatedsignaling and cilia-related disorders. Lab. Invest. 85 452 - 463    DOI : 10.1038/labinvest.3700253
Yoder B. K. , Tousson A. , Millican L. , Wu J. H. , Bugg C. E., Jr., , Schafer J. A. , Balkovetz D. F. (2002) Polaris, a protein disrupted in orpk mutant mice, is required for assembly of renal cilium. Am. J. Physiol. Renal Physiol. 282 F541 - 552
Mizuno N. , Taschner M. , Engel B. D. , Lorentzen E. (2012) Structural studies of ciliary components. J. Mol. Biol. 422 163 - 180    DOI : 10.1016/j.jmb.2012.05.040
Veland I. R. , Awan A. , Pedersen L. B. , Yoder B. K. , Christensen S. T. (2009) Primary cilia and signaling pathways in mammalian development, health and disease. Nephron Physiol. 111 39 - 53    DOI : 10.1159/000208212
Taschner M. , Bhogaraju S. , Lorentzen E. (2012) Architecture and function of IFT complex proteins in ciliogenesis. Differentiation 83 S12 - 22    DOI : 10.1016/j.diff.2011.11.001
Ishikawa H. , Marshall W. F. (2011) Ciliogenesis: building the cell's antenna. Nat. Rev. Mol. Cell Biol. 12 222 - 234    DOI : 10.1038/nrm3085
Cao Y. , Park A. , Sun Z. (2010) Intraflagellar transport proteins are essential for cilia formation and for planar cell polarity. J. Am. Soc. Nephrol. 21 1326 - 1333    DOI : 10.1681/ASN.2009091001
Pedersen L. B. , Veland I. R. , Schroder J. M. , Christensen S. T. (2008) Assembly of primary cilia. Dev. Dyn. 237 1993 - 2006    DOI : 10.1002/dvdy.21521
Hurd T. W. , Margolis B. (2009) Cystin, cilia, and cysts: unraveling trafficking determinants. J. Am. Soc. Nephrol. 20 2485 - 2486    DOI : 10.1681/ASN.2009090996
Praetorius H. A. , Spring K. R. (2001) Bending the MDCK cell primary cilium increases intracellular calcium. J. Membr. Biol. 184 71 - 79    DOI : 10.1007/s00232-001-0075-4
Yamaguchi T. , Nagao S. , Wallace D. P. , Belibi F. A. , Cowley B. D. , Pelling J. C. , Grantham J. J. (2003) Cyclic AMP activates B-Raf and ERK in cyst epithelial cells from autosomal-dominant polycystic kidneys. Kidney Int. 63 1983 - 1994    DOI : 10.1046/j.1523-1755.2003.00023.x
Lancaster M. A. , Gleeson J. G. (2009) The primary cilium as a cellular signaling center: lessons from disease. Curr. Opin. Genet. Dev. 19 220 - 229    DOI : 10.1016/j.gde.2009.04.008
Mahjoub M. R. , Trapp M. L. , Quarmby L. M. (2005) NIMA-related kinases defective in murine models of polycystic kidney diseases localize to primary cilia and centrosomes. J. Am. Soc. Nephrol. 16 3485 - 3489    DOI : 10.1681/ASN.2005080824
Rydholm S. , Zwartz G. , Kowalewski J. M. , Kamali-Zare P. , Frisk T. , Brismar H. (2010) Mechanical properties of primary cilia regulate the response to fluid flow. Am. J. Physiol. Renal Physiol. 298 F1096 - 1102    DOI : 10.1152/ajprenal.00657.2009
Cowley B. D., Jr. (2008) Calcium, cyclic AMP, and MAP kinases: dysregulation in polycystic kidney disease. Kidney Int 73 251 - 253    DOI : 10.1038/sj.ki.5002695
Choi Y. H. , Suzuki A. , Hajarnis S. , Ma Z. , Chapin H. C. , Caplan M. J. , Pontoglio M. , Somlo S. , Igarashi P. (2011) Polycystin-2 and phosphodiesterase 4C are components of a ciliary A-kinase anchoring protein complex that is disrupted in cystic kidney diseases. Proc. Natl. Acad. Sci. U.S.A. 108 10679 - 10684    DOI : 10.1073/pnas.1016214108
Pan J. , Seeger-Nukpezah T. , Golemis E. A. 2012 The role of the cilium in normal and abnormal cell cycles: emphasis on renal cystic pathologies. Cell Cell Mol. Life Sci. 2012 Jul 11 [Epub ahead of print] PubMed PMID: 22782110.
Boulter C. , Mulroy S. , Webb S. , Fleming S. , Brindle K. , Sandford R. (2001) Cardiovascular, skeletal, and renal defects in mice with a targeted disruption of the Pkd1 gene. Proc. Natl. Acad. Sci. U.S.A. 98 12174 - 12179    DOI : 10.1073/pnas.211191098
Lu W. , Peissel B. , Babakhanlou H. , Pavlova A. , Geng L. , Fan X. , Larson C. , Brent G. , Zhou J. (1997) Perinatal lethality with kidney and pancreas defects in mice with a targetted Pkd1 mutation. Nat. Genet. 17 179 - 181    DOI : 10.1038/ng1097-179
Kim K. , Drummond I. , Ibraghimov-Beskrovnaya O. , Klinger K. , Arnaout M. A. (2000) Polycystin 1 is required for the structural integrity of blood vessels. Proc. Natl. Acad. Sci. U.S.A. 97 1731 - 1736    DOI : 10.1073/pnas.040550097
Wu G. , D'Agati V. , Cai Y. , Markowitz G. , Park J. H. , Reynolds D. M. , Maeda Y. , Le T. C. , Hou H., Jr. , Kucherlapati R. , Edelmann W. , Somlo S. (1998) Somatic inactivation of Pkd2 results in polycystic kidney disease. Cell 93 177 - 188    DOI : 10.1016/S0092-8674(00)81570-6
Wang X. , Harris P. C. , Somlo S. , Batlle D. , Torres V. E. (2009) Effect of calcium-sensing receptor activation in models of autosomal recessive or dominant polycystic kidney disease, Nephrol. Dial. Transplant. 24 526 - 534    DOI : 10.1093/ndt/gfn527
Wu G. , Somlo S. (2000) Molecular genetics and mechanism of autosomal dominant polycystic kidney disease. Mol. Genet. Metab. 69 1 - 15    DOI : 10.1006/mgme.1999.2943
Wu G. , Markowitz G. S. , Li L. , D'Agati V. D. , Factor S. M. , Geng L. , Tibara S. , Tuchman J. , Cai Y. , Park J. H. , van Adelsberg J. , Hou H., Jr. , Kucherlapati R. , Edelmann W. , Somlo S (2000) Cardiac defects and renal failure in mice with targeted mutations in Pkd2. Nat. Genet. 24 75 - 78    DOI : 10.1038/71724
Thomson R. B. , Mentone S. , Kim R. , Earle K. , Delpire E. , Somlo S. , Aronson P. S. (2003) Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD. Am. J. Physiol. Renal Physiol. 285 F870 - 880
Stroope A. , Radtke B. , Huang B. , Masyuk T. , Torres V. , Ritman E. , LaRusso N. (2010) Hepato-renal pathology in pkd2ws25/- mice, an animal model of autosomal dominant polycystic kidney disease. Am. J. Pathol. 176 1282 - 1291    DOI : 10.2353/ajpath.2010.090658
Moyer J. H. , Lee-Tischler M. J. , Kwon H. Y. , Schrick J. J. , Avner E. D. , Sweeney W. E. , Godfrey V. L. , Cacheiro N. L. , Wilkinson J. E. , Woychik R. P. (1994) Candidate gene associated with a mutation causing recessive polycystic kidney disease in mice. Science 264 1329 - 1333    DOI : 10.1126/science.8191288
Pazour G. J. , Dickert B. L. , Vucica Y. , Seeley E. S. , Rosenbaum J. L. , Witman G. B. , Cole D. G. (2000) Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella. J. Cell Biol. 151 709 - 718    DOI : 10.1083/jcb.151.3.709
Pazour G. J. , San Agustin J. T. , Follit J. A. , Rosenbaum J. L. , Witman G. B. (2002) Polycystin-2 localizes to kidney cilia and the ciliary level is elevated in orpk mice with polycystic kidney disease. Curr. Biol. 12 R378 - 380    DOI : 10.1016/S0960-9822(02)00877-1
Lehman J. M. , Michaud E. J. , Schoeb T. R. , Aydin-Son Y. , Miller M. , Yoder B. K. (2008) The Oak Ridge Polycystic Kidney mouse: modeling ciliopathies of mice and men. Dev. Dyn. 237 1960 - 1971    DOI : 10.1002/dvdy.21515
Zhang Q. , Davenport J. R. , Croyle M. J. , Haycraft C. J. , Yoder B. K. (2005) Disruption of IFT results in both exocrine and endocrine abnormalities in the pancreas of Tg737(orpk) mutant mice. Lab. Invest. 85 45 - 64    DOI : 10.1038/labinvest.3700207
Banizs B. , Pike M. M. , Millican C. L. , Ferguson W. B. , Komlosi P. , Sheetz J. , Bell P. D. , Schwiebert E. M. , Yoder B. K. (2005) Dysfunctional cilia lead to altered ependyma and choroid plexus function, and result in the formation of hydrocephalus. Development 132 5329 - 5339    DOI : 10.1242/dev.02153
Cano D. A. , Murcia N. S. , Pazour G. J. , Hebrok M. (2004) Orpk mouse model of polycystic kidney disease reveals essential role of primary cilia in pancreatic tissue organization. Development 131 3457 - 3467    DOI : 10.1242/dev.01189
Jonassen J. A. , San Agustin J. , Follit J. A. , Pazour G. J. (2008) Deletion of IFT20 in the mouse kidney causes misorientation of the mitotic spindle and cystic kidney disease. J. Cell Biol. 183 377 - 384    DOI : 10.1083/jcb.200808137
Follit J. A. , Tuft R. A. , Fogarty K. E. , Pazour G. J. (2006) The intraflagellar transport protein IFT20 is associated with the Golgi complex and is required for cilia assembly. Mol. Biol. Cell 17 3781 - 3792    DOI : 10.1091/mbc.E06-02-0133
Jonassen J. A. , SanAgustin J. , Baker S. P. , Pazour G. J. (2012) Disruption of IFT complex A causes cystic kidneys without mitotic spindle misorientation. J. Am. Soc. Nephrol. 23 641 - 651    DOI : 10.1681/ASN.2011080829
Marszalek J. R. , Ruiz-Lozano P. , Roberts E. , Chien K. R. , Goldstein L. S. (1999) Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II. Proc. Natl. Acad. Sci. U.S.A. 96 5043 - 5048    DOI : 10.1073/pnas.96.9.5043
Takeda S. , Yonekawa Y. , Tanaka Y. , Okada Y. , Nonaka S. , Hirokawa N. (1999) Left-right asymmetry and kinesin superfamily protein KIF3A: new insights in determination of laterality and mesoderm induction by kif3A-/- mice analysis. J. Cell Biol. 145 825 - 836    DOI : 10.1083/jcb.145.4.825
Lin F. , Hiesberger T. , Cordes K. , Sinclair A. M. , Goldstein L. S. , Somlo S. , Igarashi P. (2003) Kidney-specific inactivation of the KIF3A subunit of kinesin-II inhibits renal ciliogenesis and produces polycystic kidney disease. Proc. Natl. Acad. Sci. U.S.A. 100 5286 - 5291    DOI : 10.1073/pnas.0836980100
Tao B. , Bu S. , Yang Z. , Siroky B. , Kappes J. C. , Kispert A. , Guay-Woodford L. M. (2009) Cystin localizes to primary cilia via membrane microdomains and a targeting motif. J. Am. Soc. Nephrol. 20 2570 - 2580    DOI : 10.1681/ASN.2009020188
Yoder B. K. , Hou X. , Guay-Woodford L. M. (2002) The polycystic kidney disease proteins, polycystin-1, polycystin-2, polaris, and cystin, are co-localized in renal cilia. J. Am. Soc. Nephrol. 13 2508 - 2516    DOI : 10.1097/01.ASN.0000029587.47950.25
Chiu M. G. , Johnson T. M. , Woolf A. S. , Dahm-Vicker E. M. , Long D. A. , Guay-Woodford L. , Hillman K. A. , Bawumia S. , Venner K. , Hughes R. C. , Poirier F. , Winyard P. J (2006) Galectin-3 associates with the primary cilium and modulates cyst growth in congenital polycystic kidney disease. Am. J. Pathol. 169 1925 - 1938    DOI : 10.2353/ajpath.2006.060245
Hou X. , Mrug M. , Yoder B. K. , Lefkowitz E. J. , Kremmidiotis G. , D'Eustachio P. , Beier D. R. , Guay-Woodford L. M. (2002) Cystin, a novel cilia-associated protein, is disrupted in the cpk mouse model of polycystic kidney disease. J. Clin. Invest. 109 533 - 540    DOI : 10.1172/JCI0214099
Preminger G. M. , Koch W. E. , Fried F. A. , McFarland E. , Murphy E. D. , Mandell J. (1982) Murine congenital polycystic kidney disease: a model for studying development of cystic disease. J. Urol. 127 556 - 560
Cowley B. D., Jr. , Smardo F. L., Jr. , Grantham J. J. , Calvet J. P. (1987) Elevated c-myc protooncogene expression in autosomal recessive polycystic kidney disease. Proc. Natl. Acad. Sci. U.S.A. 84 8394 - 8398    DOI : 10.1073/pnas.84.23.8394
Cowley B. D., Jr. , Chadwick L. J. , Grantham J. J. , Calvet J. P. (1991) Elevated proto-oncogene expression in polycystic kidneys of the C57BL/6J (cpk) mouse. J. Am. Soc. Nephrol. 1 1048 - 1053
Nakamura T. , Ebihara I. , Nagaoka I. , Tomino Y. , Nagao S. , Takahashi H. , Koide H. (1993) Growth factor gene expression in kidney of murine polycystic kidney disease. J. Am. Soc. Nephrol. 3 1378 - 1386
Rocco M. V. , Neilson E. G. , Hoyer J. R. , Ziyadeh F. N. (1992) Attenuated expression of epithelial cell adhesion molecules in murine polycystic kidney disease. Am. J. Physiol. 262 F679 - 686
Rankin C. A. , Suzuki K. , Itoh Y. , Ziemer D. M. , Grantham J. J. , Calvet J. P. , Nagase H. (1996) Matrix metalloproteinases and TIMPS in cultured C57BL/6J-cpk kidney tubules. Kidney Int. 50 835 - 844    DOI : 10.1038/ki.1996.383
Winyard P. , Jenkins D. (2011) Putative roles of cilia in polycystic kidney disease. Biochim. Biophys. Acta. 1812 1256 - 1262    DOI : 10.1016/j.bbadis.2011.04.012
Gerdes J. M. , Davis E. E. , Katsanis N. (2009) The vertebrate primary cilium in development, homeostasis, and disease. Cell 137 32 - 45    DOI : 10.1016/j.cell.2009.03.023
Yokoyama T. , Copeland N. G. , Jenkins N. A. , Montgomery C. A. , Elder F. F. , Overbeek P. A. (1993) Reversal of left-right asymmetry: a situs inversus mutation. Science 260 679 - 682    DOI : 10.1126/science.8480178
Guay-Woodford L. M. (2003) Murine models of polycystic kidney disease: molecular and therapeutic insights. Am. J. Physiol. Renal Physiol. 285 F1034 - 1049
Morgan D. , Eley L. , Sayer J. , Strachan T. , Yates L. M. , Craighead A. S. , Goodship J. A. (2002) Expression analyses and interaction with the anaphase promoting complex protein Apc2 suggest a role for inversin in primary cilia and involvement in the cell cycle. Human Mol. Genet. 11 3345 - 3350    DOI : 10.1093/hmg/11.26.3345
Phillips C. L. , Miller K. J. , Filson A. J. , Nurnberger J. , Clendenon J. L. , Cook G. W. , Dunn K. W. , Overbeek P. A. , Gattone V. H. 2nd , Bacallao R. L. (2004) Renal cysts of inv/inv mice resemble early infantile nephronophthisis. J. Am. Soc. Nephrol. 15 1744 - 1755    DOI : 10.1097/01.ASN.0000131520.07008.B3
Sugiyama N. , Tsukiyama T. , Yamaguchi T. P. , Yokoyama T. (2011) The canonical Wnt signaling pathway is not involved in renal cyst development in the kidneys of inv mutant mice. Kidney Int. 79 957 - 965    DOI : 10.1038/ki.2010.534
Mykytyn K. , Mullins R. F. , Andrews M. , Chiang A. P. , Swiderski R. E. , Yang B. , Braun T. , Casavant T. , Stone E. M. , Sheffield V. C. (2004) Bardet-Biedl syndrome type 4 (BBS4)-null mice implicate Bbs4 in flagella formation but not global cilia assembly. Proc. Natl. Acad. Sci. U.S.A. 101 8664 - 8669    DOI : 10.1073/pnas.0402354101
Li J. B. , Gerdes J. M. , Haycraft C. J. , Fan Y. , Teslovich T. M. , May-Simera H. , Li H. , Blacque O. E. , Li L. , Leitch C. C. , Lewis R. A. , Green J. S. , Parfrey P. S. , Leroux M. R. , Davidson W. S. , Beales P. L. , Guay-Woodford L. M. , Yoder B. K. , Stormo G. D. , Katsanis N. , Dutcher S. K. (2004) Comparative genomics identifies a flagellar and basal body proteome that includes the BBS5 human disease gene. Cell 117 541 - 552    DOI : 10.1016/S0092-8674(04)00450-7
Ansley S. J. , Badano J. L. , Blacque O. E. , Hill J. , Hoskins B. E. , Leitch C. C. , Kim J. C. , Ross A. J. , Eichers E. R. , Teslovich T. M. , Mah A. K. , Johnsen R. C. , Cavender J. C. , Lewis R. A. , Leroux M. R. , Beales P. L. , Katsanis N. (2003) Basal body dysfunction is a likely cause of pleiotropic Bardet-Biedl syndrome. Nature 425 628 - 633    DOI : 10.1038/nature02030
Kim J. C. , Badano J. L. , Sibold S. , Esmail M. A. , Hill J. , Hoskins B. E. , Leitch C. C. , Venner K. , Ansley S. J. , Ross A. J. , Leroux M. R. , Katsanis N. , Beales P. L. (2004) The Bardet-Biedl protein BBS4 targets cargo to the pericentriolar region and is required for microtubule anchoring and cell cycle progression. Nat. Genet. 36 462 - 470    DOI : 10.1038/ng1352
Nachury M. V. , Loktev A. V. , Zhang Q. , Westlake C. J. , Peranen J. , Merdes A. , Slusarski D. C. , Scheller R. H. , Bazan J. F. , Sheffield V. C. , Jackson P. K. (2007) A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis. Cell 129 1201 - 1213    DOI : 10.1016/j.cell.2007.03.053
Nishimura D. Y. , Fath M. , Mullins R. F. , Searby C. , Andrews M. , Davis R. , Andorf J. L. , Mykytyn K. , Swiderski R. E. , Yang B. , Carmi R. , Stone E. M. , Sheffield V. C (2004) Bbs2-null mice have neurosensory deficits, a defect in social dominance, and retinopathy associated with mislocalization of rhodopsin. Proc. Natl. Acad. Sci. U.S.A. 101 16588 - 16593    DOI : 10.1073/pnas.0405496101
Liu S. , Lu W. , Obara T. , Kuida S. , Lehoczky J. , Dewar K. , Drummond I. A. , Beier D. R. (2002) A defect in a novel Nek-family kinase causes cystic kidney disease in the mouse and in zebrafish. Development 129 5839 - 5846    DOI : 10.1242/dev.00173
Sohara E. , Luo Y. , Zhang J. , Manning D. K. , Beier D. R. , Zhou J. (2008) Nek8 regulates the expression and localization of polycystin-1 and polycystin-2. J. Am. Soc. Nephrol. 19 469 - 476    DOI : 10.1681/ASN.2006090985
Bowers A. J. , Boylan J. F. (2004) Nek8, a NIMA family kinase member, is overexpressed in primary human breast tumors. Gene 328 135 - 142    DOI : 10.1016/j.gene.2003.12.002
Smith L. A. , Bukanov N. O. , Husson H. , Russo R. J. , Barry T. C. , Taylor A. L. , Beier D. R. , Ibraghimov-Beskrovnaya O. (2006) Development of polycystic kidney disease in juvenile cystic kidney mice: insights into pathogenesis, ciliary abnormalities, and common features with human disease. J. Am. Soc. Nephrol. 17 2821 - 2831    DOI : 10.1681/ASN.2006020136