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Biosynthesis of Novel Glucosides Geldanamycin Analogs by Enzymatic Synthesis
Biosynthesis of Novel Glucosides Geldanamycin Analogs by Enzymatic Synthesis
Qiang Huo , Hong-Mei Li , Jae Kyoung Lee , Jing Li , Tao Ma , Xinyu Zhang , Yiqun Dai , Young-Soo Hong , Cheng-Zhu Wu
Journal of Microbiology and Biotechnology. 2016. Jan, 26(1): 56-60
DOI : http://dx.doi.org/10.4014/jmb.1508.08069
Copyright © 2016, The Korean Society For Microbiology And Biotechnology
  • Received : August 24, 2015
  • Accepted : October 09, 2015
  • Published : January 28, 2016
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Qiang Huo
Hong-Mei Li
Jae Kyoung Lee
Jing Li
Tao Ma
Xinyu Zhang
Yiqun Dai
Young-Soo Hong
Cheng-Zhu Wu
About the Authors
Qiang Huo
Faculty of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
Hong-Mei Li
Faculty of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
Jae Kyoung Lee
Chemical Biology Research Center, KRIBB, Cheongju 28116, Republic of Korea
Jing Li
Faculty of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
Tao Ma
Faculty of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
Xinyu Zhang
Faculty of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
Yiqun Dai
Faculty of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
Young-Soo Hong
Chemical Biology Research Center, KRIBB, Cheongju 28116, Republic of Korea
hongsoo@kribb.re.kr
Cheng-Zhu Wu
Faculty of Pharmacy, Bengbu Medical College, Bengbu, Anhui 233030, P.R. China
hongsoo@kribb.re.kr
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Abstract
Keywords

Abstract
Two new glucosides ( 1 and 2 ) of geldanamycin (GA) analogs were obtained from in vitro glycosylation by UDP-glycosyltransferase (YjiC). Based on spectroscopic (HR-ESI-MS, 1D, and 2D-NMR) analyses, the glucosides were elucidated as 4,5-dihydro-7- O -descarbamoyl-7-hydroxyl GA-7- O -β-D-glucoside ( 1 ) and ACDL3172-18- O -β-D-glucoside ( 2 ). Furthermore, the water solubility of compounds 1 and 2 was about 215.2 and 90.7 times higher respectively, than that of the substrates. Among compounds 1 - 4 , only 3 showed weak antiproliferative activity against four human tumor cell lines: MDA-MB-231, SMMC7721, HepG2, and SW480 (IC 50 : 13.6, 15.1, 31.8, and 22.7 μM, respectively).
Keywords
Geldanamycin analogs ;  glycosyltransferase ;  water solubility ;  cytotoxicity
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Acknowledgements
This work was financially supported by the National Natural Science Foundation of China (Grant No. 81302671); the Natural Science Foundation of Anhui Province (Grant No. 1408085QH162); and the Education Department of Anhui Natural Science Research Project China (Grant No. KJ2015A274). This work was also supported in part by a grant from the KRIBB Research Initiative Program.
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Citing 'Biosynthesis of Novel Glucosides Geldanamycin Analogs by Enzymatic Synthesis '
@article{ E1MBA4_2016_v26n1_56} ,title={Biosynthesis of Novel Glucosides Geldanamycin Analogs by Enzymatic Synthesis} ,volume={1} , url={http://dx.doi.org/10.4014/jmb.1508.08069}, DOI={10.4014/jmb.1508.08069} , number= {1} , journal={Journal of Microbiology and Biotechnology} , publisher={The Korean Society for Applied Microbiology and Biotechnology} , author={Huo, Qiang and Li, Hong-Mei and Lee, Jae Kyoung and Li, Jing and Ma, Tao and Zhang, Xinyu and Dai, Yiqun and Hong, Young-Soo and Wu, Cheng-Zhu} , year={2016} , month={Jan}