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Development of a Rapid and Accurate Identification Method for Citrobacter Species Isolated from Pork Products Using a Matrix-Assisted Laser-Desorption Ionization Time-of-Flight Mass Spectrometry (MALDITOF MS)S
Development of a Rapid and Accurate Identification Method for Citrobacter Species Isolated from Pork Products Using a Matrix-Assisted Laser-Desorption Ionization Time-of-Flight Mass Spectrometry (MALDITOF MS)S
Journal of Microbiology and Biotechnology. 2015. Sep, 25(9): 1537-1541
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : March 20, 2015
  • Accepted : May 24, 2015
  • Published : September 28, 2015
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About the Authors
Hye-Lim Kwak
Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea
Sun-Kyung Han
Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea
Sunghoon Park
Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea
Si Hong Park
Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA
Jae-Yong Shim
Department of Food & Biotechnology, Hankyong National University, Anseong 456-749, Republic of Korea
Mihwa Oh
National Institute of Animal Science, Rural Development Administration, Suwon 441-706, Republic of Korea
Steven C. Ricke
Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville, AR 72704, USA
Hae-Yeong Kim
Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea
hykim@khu.ac.kr

Abstract
Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDITOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.
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Acknowledgements
This work was supported by a grant from the Agenda Program (PJ009237) of the Rural Development Administration in the Republic of Korea.
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