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Probiotic Mixture KF Attenuates Age-Dependent Memory Deficit and Lipidemia in Fischer 344 Rats
Probiotic Mixture KF Attenuates Age-Dependent Memory Deficit and Lipidemia in Fischer 344 Rats
Journal of Microbiology and Biotechnology. 2015. Sep, 25(9): 1532-1536
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : May 06, 2015
  • Accepted : May 14, 2015
  • Published : September 28, 2015
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About the Authors
Jin-Ju Jeong
Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea
Kyung-Ah Kim
Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea
Young-Tae Ahn
R&BD Center, Korea Yakult Co., Ltd., Yongin 449-901, Republic of Korea
Jae-Hun Sim
R&BD Center, Korea Yakult Co., Ltd., Yongin 449-901, Republic of Korea
Jae-Yeon Woo
Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea
Chul-Sung Huh
Graduate School of International Agricultural Technology/GBST, Seoul National University, Gangwon 232-916, Republic of Korea
Dong-Hyun Kim
Department of Life and Nanopharmaceutical Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea
dhkim@khu.ac.kr

Abstract
To investigate the memory-enhancing effect of lactic acid bacteria, we selected the probiotic mixture KF, which consisted of Lactobacillus plantarum KY1032 and Lactobacillus curvatus HY7601 (1 × 10 11 CFU/g of each strain), and investigated its antilipidemic and memoryenhancing effects in aged Fischer 344 rats. KF (1 × 10 10 CFU/rat/day), which was administered orally once a day (6 days per week) for 8 weeks, significantly inhibited age-dependent increases of blood triglyceride and reductions of HDL cholesterol ( p < 0.05). KF restored agereduced spontaneous alternation in the Y-maze task to 94.4% of that seen in young rats ( p < 0.05). KF treatment slightly, but not significantly, shortened the escape latency daily for 4 days. Oral administration of KF restored age-suppressed doublecortin and brain-derived neurotrophic factor expression in aged rats. Orally administered KF suppressed the expression of p16, p53, and cyclooxygenase-2, the phosphorylation of Akt and mTOR, and the activation of NF-κB in the hippocampus of the brain. These findings suggest that KF may ameliorate age-dependent memory deficit and lipidemia by inhibiting NF-κB activation.
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Acknowledgements
This study was supported by grants from the Bio & Medical Technology Development Program (2013M3A9B6076413) of the National Research Foundation (NRF) funded by the Korean government (MSIP).
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