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An Efficient PEG/CaCl<sub>2</sub>-Mediated Transformation Approach for the Medicinal Fungus Wolfiporia cocos
An Efficient PEG/CaCl2-Mediated Transformation Approach for the Medicinal Fungus Wolfiporia cocos
Journal of Microbiology and Biotechnology. 2015. Sep, 25(9): 1528-1531
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : January 20, 2015
  • Accepted : May 19, 2015
  • Published : September 28, 2015
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About the Authors
Qiao Sun
College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China
Wei Wei
Institute for Interdisciplinary Research, Jianghan University, Wuhan 430056, P.R. China
Juan Zhao
College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China
Jia Song
College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China
Fang Peng
College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China
Shaopeng Zhang
College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China
Yonglian Zheng
College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China
Ping Chen
College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China
Wenjun Zhu
College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P.R. China
zhuwenjun2002@163.com

Abstract
Sclerotia of Wolfiporia cocos are of medicinal and culinary value. The genes and molecular mechanisms involved in W. cocos sclerotial formation are poorly investigated because of the lack of a suitable and reproducible transformation system for W. cocos . In this study, a PEG/CaCl 2 -mediated genetic transformation system for W. cocos was developed. The promoter Pgpd from Ganoderma lucidum effectively drove expression of the hygromycin B phosphotransferase gene in W. cocos , and approximately 30 transformants were obtained per 10 μg DNA when the protoplast suspension density was 10 6 protoplasts/ml. However, no transformants were obtained under the regulation of the PtrpC promoter from Aspergillus nidulans .
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Acknowledgements
We are grateful to Pro Daohong Jiang, College of Plant Science and Technology, Huazhong Agricultural University, for the vectors donation. This research was supported by the State of Traditional Chinese Medicine Industry Research Program (No. 201107009).
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