γ-Aminobutyric Acid (GABA) Production and Angiotensin-I Converting Enzyme (ACE) Inhibitory Activity of Fermented Soybean Containing Sea Tangle by the Co-Culture of Lactobacillus brevis with Aspergillus oryzae
γ-Aminobutyric Acid (GABA) Production and Angiotensin-I Converting Enzyme (ACE) Inhibitory Activity of Fermented Soybean Containing Sea Tangle by the Co-Culture of Lactobacillus brevis with Aspergillus oryzae
Journal of Microbiology and Biotechnology. 2015. Sep, 25(8): 1315-1320
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : December 16, 2014
  • Accepted : April 04, 2015
  • Published : September 28, 2015
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About the Authors
Eun Kyeong, Jang
Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 151-742, Republic of Korea
Nam Yeun, Kim
Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 151-742, Republic of Korea
Hyung Jin, Ahn
Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 151-742, Republic of Korea
Geun Eog, Ji
Research Institute, Bifido Co., Ltd., Hongchun 250-804, Republic of Korea

To enhance the γ -aminobutyric acid (GABA) content, the optimized fermentation of soybean with added sea tangle extract was evaluated at 30℃ and pH 5.0. The medium was first inoculated with Aspergillus oryzae strain FMB S46471 and fermented for 3 days, followed by the subsequent inoculation with Lactobacillus brevis GABA 100. After fermentation for 7 days, the fermented soybean showed approximately 1.9 g/kg GABA and exhibited higher ACE inhibitory activity than the traditional soybean product. Furthermore, several peptides in the fraction containing the highest ACE inhibitory activity were identified. The novel fermented soybean enriched with GABA and ACE inhibitory components has great pharmaceutical and functional food values.
γ -Aminobutyric acid (GABA), which is known as a neurotransmitter in the central nervous system, has various physiological functions in animals and humans, including antihypertensive activity [3 , 4] . Moreover, angiontensin-I converting enzyme (ACE)-inhibitory activity can be also related to enzymatic proteolysis and can regulate the pressure of blood by binding with ACE [15] . The aim of this study was to develop a novel functional fermented soybean with both high GABA and ACE inhibition characteristics. To increase the GABA content and ACE inhibitory activity in the fermented soybeans, optimization of the soybean fermentation process was conducted for several fermentation conditions. Sea tangle ( Saccharina japonica ), known as a seaweed that has plentiful glutamic acid, was used to provide a natural source of glutamate [12] . ACE inhibitory activities were analyzed at each stage, from the fermentation to the digestion of the fermented soybeans, and the molecular masses and amino acid sequences of the peptides that were present in the ACE inhibitory fraction were identified.
Optimization of GABA Production in Fermented Soybean
Basal steamed soybeans were prepared as follows. Soybeans crushed by a blender were added to sea tangle extract (STE). STE was prepared by autoclaving the mixture with sea tangle powder and distilled water, and filtered through filter paper. Next, soybeans containing STE were sterilized by autoclaving at 121℃ for 15 min. After fermentation, the amino acids and GABA were assessed by TLC and HPLC [6 , 8] . Three microorganisms were used for the fermentation; A. oryzae FMB S46471 and B. subtilis natto , which were derived from meju , and L. brevis GABA 100, which was isolated from kimchi , as previously reported [7 , 9] . A. oryzae FMB S46471 was inoculated at 5 × 10 4 spores/ml at the beginning of the fermentation, whereas B. subtilis natto and L. brevis GABA 100 were inoculated at the 1% (v/v, 10 8 CFU/ml) level at the beginning of fermentation, or after 3days of fermentation. GABA production was observed when L. brevis GABA 100 was cultured. The fermented soybean cultured with B. subtilis natto or co-cultured with B. subtilis natto consumed the glutamate; thus, GABA was minimally produced even in the presence of L. brevis GABA 100 (data not shown). These results were similar to the usual fermentation process with A. oryzae and B. subtilis [5] .
Various combinations of the soybean, sea tangle, and water were composed according to Table 1 . Groups G1 to G9 comprised the various compositions of soybean and sea tangle in 5 g quantities; Groups G10 and G11 were compared with G1, and then the optimal fermentative conditions for GABA production were selected based on TLC analysis. As a result, GABA was produced in greater quantities by increasing the soybean content compared with sea tangle ( Fig. 1 A). The fermented soybean added with sea tangle extract showed lower levels of GABA than that added with sea tangle powder. The sea tangle component present in the powder but excluded in the extract may have contributed to the elevation of GABA production. For instance, group G5 (composed only with STP) contained GABA after the fermentation, whereas in group G9 (composed only with STE), the pattern of GABA was not detected. In Fig. 1 B, the pattern of glutamate in groups G10 and G11 by the third day declined compared with the beginning of the fermentation period. In other words, the glutamate from the sea tangle was not used to produce GABA during fermentation by the third day, which was partially consumed by the microbes. Furthermore, the effects of temperature (25℃, 30℃, and 37℃), pH (4.0 to 6.0), inoculum age, and several carbohydrates were investigated. The optimal fermentative conditions were found to be 30℃, pH 5.0, with no added carbohydrates based on the TLC (data not shown). A. oryzae FMB S46471, which was inoculated at the beginning of fermentation, might have extended the period of protein hydrolysis. L. brevis GABA 100, which has GABA-producing activity, showed the highest GABA content when inoculated on the third day. Moreover, as shown in Fig. 1 C, adding the sea tangle to the soybean had a beneficial function in regulating the pH of the soybean medium during fermentation. Groups G10 and G11 were able to maintain a pH of approximately 6.0 or below, whereas the pH of G1 increased. In this study, GAD of L. brevis played an important role in converting glutamate to GABA. Therefore, the sea tangle was able to lower and maintain the pH to the nearly optimal condition for GAD activity and GABA production.
The various medium compositions with different ratios of soybean, sea tangle, and water, used for the fermentative production of GABA.
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SB, soybean; STP, dried sea tangle powder; STE, sea tangle extract; DW, distilled water. STE was prepared by autoclaving a mixture of sea tangle powder and distilled water, and was filtered through filter paper. a Groups G1, G10, and G11 were selected to measure glutamate and GABA, both of which were assessed by HPLC. Other groups were not evaluated, because they showed lower GABA than G1, G10, and G11 analyzed by the TLC method (data not shown).
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Effects of various media compositions on the production of GABA. The pattern of amino acid production depending on medium composition (A and B) and pH (C) (circles, G1; diamonds, G10; squares, G11). M, MSG; G, GABA.
The selected fermentation conditions based on TLC analysis were those found in group G1 (as a control group), and then G10 and G11, which contained the added sea tangle and the highest soybean composition. The fermented soybean–containing STP cultured under optimal conditions produced 1.9 g/kg of GABA.
Measurement of the ACE Inhibitory Activity of Fermented Soybean
The ACE-inhibition effect of fermented soybeans was determined in vitro using a previously described method [10] . As shown in Fig. 2 A, ACE inhibitory activities were increased in the soybeans fermented by A. oryzae FMB S46471 compared with those fermented by L. brevis GABA 100 or B. subtilis natto . Only group A0G3 showed a high level of GABA and ACE inhibition, which was fermented with A. oryzae FMB S46471 at the beginning of fermentation and then inoculated with L. brevis GABA 100 at 3 days after fermentation. Thus, subsequent experiments were conducted by using the inoculum combination of A0G3 to increase GABA production and ACE inhibitory activity simultaneously.
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ACE inhibitory activities of fermented soybeans depending on the inoculation combination (A), the addition of sea tangle (B), and the stage of fermentation and digestion (C). (A) A, A. oryzae FMB S46471; G, L. brevis GABA 100; B, B. subtilis natto. The inoculation time of each microorganism is written on the right of the microorganism abbreviation in the fermented groups. (B), (C) Refer to Table 1 for the details of each fermentation group; data are expressed as averages in triplicate.
When L. brevis was inoculated into the soybean, the pH typically decreased to approximately 4.3 within 24 h, whereas A. oryzae and B. subtilis increased the pH by hydrolyzing the soybean protein. The change of pH until the third day and the pattern of amino acid production in group A0G0 were similar to that of G0 (data not shown). Production of amino acids and GABA was affected by a pH below 5.0, which was able to inactivate or decrease the activities of enzymes, such as protease and GAD. Release of amino acids by A. oryzae was observed at the third day after inoculation, and the pH failed to increase, as presented in Fig. 1 C. This means that A. oryzae needed 3days to grow and produce proteases after inoculation. Thus, the ACE inhibitory activity of A. oryzae could be decreased by the lower pH when L. brevis was inoculated at the beginning of fermentation.
In group A0G3, the pH ranged from 5.5 to 6.5 for 3 days, which was when A. oryzae was growing during the fermentation. Then, L. brevis lowered the pH to 5.0-6.0, at which time GAD efficiently converted glutamate into GABA. Likewise, this pH range might not affect the inhibition of the growth and the protease activity of A. oryzae .
In the A. oryzae FMB S46471 and L. brevis GABA 100 cocultured fermented soybean, the combination of soybean and sea tangle in the fermented soybeans also affected ACE inhibition, and the results showed that the ACE inhibitory activities were positively proportional to the level of GABA production. The diluted groups of G10 and G11 (at the ratio of 1:100 (v/v)) were above 50% ( Fig. 2 B). In comparison, the diluted Korean traditional fermented soybeans (at the ratio of 1:100 (v/v)) showed 22–31% ACE inhibitory activity in this study. The addition of STE (groups G6-G9) was less effective on the ACE inhibitory activity than STP addition (groups G2-G5). ACE inhibition was correlated with a high degree of hydrolysis [2] , and the amount of GABA was influenced by the amount of glutamate produced from protein sources. The in vitro ACE inhibitory activity of the fermented soybean (at a dilution ratio of 1:100) was evaluated by its in vitro treatment with various digestive enzymes (pepsin, trypsin, and α-chymotrypsin) and the subsequent estimation of the ACE inhibition activities.
In all tested samples of fermented soybean with various combinations of soybean and sea tangle, ACE was increased by fermentation, but it tended to be decreased by digestive enzymes. This suggests that ACE inhibitors generated from fermented soybeans might be hydrolyzed by gastrointestinal digestive enzymes, such as pepsin, trypsin, and α-chymotrypsin. From the results of these comparisons, the fermentation of soybeans was shown to be a beneficial process that improved bioactivity, such as GABA production and ACE inhibitors, although fermented soybean hydrolysate decreased ACE inhibition compared with prior digestion. Several studies reported that fermented soybeans, such as soy milk and Korean soybean paste, lowered the blood pressure in in vivo models, although they were fermented under slightly different conditions [13 , 14] .
Purification and Identification of Peptides in the High ACE Inhibitory Fraction
The fermented soybean G10 hydrolysate (FSH) was fractionated using UF membrane filters. The ACE inhibitory activity of the fraction below the 3kDa MW cut-off showed the lowest IC 50 (11.69 µg/ml). Then, the identification of peptides in the ACE inhibitory fraction having the highest activity was assessed by nano-LC-ESI-MS/MS. The MS/ MS spectra of the peptide fractions below 3kDa indicated nine novel peptides derived from soybeans that had not been previously reported ( Table 2 ).
Identified peptides in fermented soybean hydrolysate (FSHa) fractionated below 3 kDa.
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a Fermented soybean hydrolysate was treated with pepsin, trypsin, and α-chymotrypsin. Pepsin was used at a ratio of 1:100 (w/w) (enzyme:substrate), and pepsin hydrolysis was proceeded at pH 2.0 for 5 h. Trypsin and α-chymotrypsin were each used at a ratio of 1:200 (w/w) (enzyme:substrate). Trypsin and α-chymotrypsin were added into previous pepsin hydrolysate, and hydrolysis was proceeded at pH 7.5–8.0 for 5 h.
A peptide, WAMLGALGCVFPELLARNGVKFGEASWFK, contains hydrophobic-rich amino acids at the N-terminus and Trp and Phe at the C-terminus as a potent inhibitor, which may easily bind on the structure of ACE [1] . In addition, the peptides VFDGELQEGR and LQESVIVEISKK were mentioned in a patent indicating that several polypeptides or polypeptide fragments that included these sequences could be isolated from soybean oil body– associated proteins; they may be useful for the treatment or prevention of cardiovascular disease because of their suppression of cholesterol uptake by Caco-2 cells in a dose-dependent manner [11] . Therefore, the fermented soybean samples manufactured in this study are expected to regulate blood pressure based on the enriched GABA content and the increased ACE inhibitory activity.
This work was supported by the Next Generation BioGreen 21 Program (No. PJ0112302015), Rural Development Administration Republic of Korea.
Balti R , Nedjar-Arroume N , Bougatef A , Guillochon D , Nasri M 2010 Three novel angiotensin I-converting enzyme (ACE) inhibitory peptides from cuttlefish (Sepia officinalis) using digestive proteases. Food Res. Int. 43 1136 - 1143    DOI : 10.1016/j.foodres.2010.02.013
Gonalea-Gonzalea CR , uohy KM , Jauregi P 2011 Production of angiotensin-I-converting enzyme (ACE) inhibitory activity in milk fermented with probiotic strains: effects of calcium, pH and peptides on the ACE-inhibitory activity. Int. Dairy J. 21 615 - 622    DOI : 10.1016/j.idairyj.2011.04.001
Hayakaw K , Kimura M , Kasaha K , Matsumoto K , Sansawa H , Yamori Y 2004 Effect of aγ-aminobutyric acid-enriched dairy product on the blood pressure of spontaneously hypertensive and normotensive Wistar–Kyoto rats. Br. J. Nutr. 92 411 - 417    DOI : 10.1079/BJN20041221
Inoue K , Shirai T , Ochiai H , Kasao M , Hayakawa K , Kimura M , Sansawa H 2003 Blood-pressure-lowering effect of a novel fermented milk containingγ-aminobutyric acid (GABA) in mild hypertensives. Eur. J. Clin. Nutr. 57 490 - 495    DOI : 10.1038/sj.ejcn.1601555
Jo SJ , Hong CO , Yang SY , Choi KK , Kim HK , Yang H , Lee KW 2011 Changes in contents ofγ-aminobutyric acid (GABA) and isoflavones in traditional Koreandoenjangby ripening periods. J. Korean Soc. Food Sci. Nutr. 40 557 - 564    DOI : 10.3746/jkfn.2011.40.4.557
Kim JA , Park MS , Kang SA , Ji GE 2014 Production ofγ-aminobutyric acid during fermentation ofGastrodia elataBl by co-culture ofLactobacillus brevisGABA 100 withBifidobacterium bifidumBGN4. Food Sci. Biotechnol. 23 459 - 466    DOI : 10.1007/s10068-014-0063-y
Kim JY , Lee MY , Ji GE , Lee YS , Hwang KT 2009 Production ofγ-aminobutyric acid in black raspberry juice during fermentation byLactobacillus brevisGABA100. Int. J. Food Microbiol. 130 12 - 16    DOI : 10.1016/j.ijfoodmicro.2008.12.028
Kim NY , Ji GE 2014 Characterization of soybean fermented by aflatoxin non-producingAspergillus oryzaeandγ-aminobutyric acid producingLactobacillus brevis. J. Korean Soc. Appl. Biol. Chem. 57 703 - 708    DOI : 10.1007/s13765-014-4227-5
Kim NY , Lee JH , Lee IH , Ji GE 2014 An evaluation of aflatoxin and cyclopiazonic acid production inAspergillus oryzae. J. Food Prot. 77 1010 - 1016    DOI : 10.4315/0362-028X.JFP-13-448
Liu CF , Tung YT , Wu CL , Lee BH , Hsu WH , Pan TM 2011 Antihypertensive effects ofLactobacillus-fermented milk orally administered to spontaneously hypertensive rats. J. Agric. Food Chem. 59 4537 - 4543    DOI : 10.1021/jf104985v
Monsanto Technology Lic 2003 Oil body associated protein compositions and methods of use thereof for reducing the risk of cardiovascular disease. US 10/511,669 2003
Mouritsen OG , Williams L , Bjerregaard R , Duelund L 2012 Seaweeds forumamiflavour in the New Nordic Cuisine. Flavour 1 4 -    DOI : 10.1186/2044-7248-1-4
Shin ZI , Yu R , Park SA , Chung DK , Ahn CW , Nam HS 2001 His-His-Leu, an angiotensin I converting enzyme inhibitory peptide derived from Korean soybean paste, exerts antihypertensive activityin vivo J. Agric. Food Chem. 49 3004 - 3009    DOI : 10.1021/jf001135r
Tsai JS , Lin YS , Pan BS , Chen TJ 2006 Antihypertensive peptides and aminobutyric acid from prozyme 6 facilitated lactic acid bacteria fermentation of soymilk. Process Biochem. 41 1282 - 1288    DOI : 10.1016/j.procbio.2005.12.026
Weber KT , Brilla CG 1991 Pathological hypertrophy and cardiac interstitium fibrosis and renin-angiotensin-aldosterone system. Circulation 83 6 -    DOI : 10.1161/01.CIR.83.6.1849