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Identification of the σ70-Dependent Promoter Controlling Expression of the ansPAB Operon of the Nitrogen-Fixing Bacterium Rhizobium etliS
Identification of the σ70-Dependent Promoter Controlling Expression of the ansPAB Operon of the Nitrogen-Fixing Bacterium Rhizobium etliS
Journal of Microbiology and Biotechnology. 2015. Aug, 25(8): 1241-1245
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : March 04, 2015
  • Accepted : April 02, 2015
  • Published : August 28, 2015
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About the Authors
Angélica Moreno-Enríquez
Escuela de Medicina, Universidad Marista de Mérida, Yucatán, México
Zahaed Evangelista-Martínez
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco AC, Unidad Sureste, Parque Científico de Yucatán, Yucatán, México
Luis Servín-González
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, D.F., México
María Elena Flores-Carrasco
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, D.F., México
mflores@biomedicas.unam.mx

Abstract
The aim of the present work was to examine the putative promoter region of the operon ansPAB and to determine the general elements required for the regulation of transcriptional activity. The transcriptional start site of the ansPAB promoter was determined by using highresolution S1-nuclease mapping. Sequence analysis of this region showed -10 and -35 elements, which were consistent with consensus sequences for R. etli promoters that are recognized by the major form of RNA polymerase containing the σ 70 transcription factor. Mutation studies affecting several regions located upstream of the transcriptional start site confirmed the importance of these elements on transcriptional expression.
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Acknowledgements
We are grateful to Dr. María de Lourdes Girard for the gift of pBBMCS53 and Dr. Miguel A. Ramírez-Romero for technical help. A.M-E. was supported by a M.Sc. scholarship (168696) obtained from the Consejo Nacional de Ciencia y Tecnología, México.
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