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Cloning, Overexpression, and Characterization of a Metagenome-Derived Phytase with Optimal Activity at Low pH S
Cloning, Overexpression, and Characterization of a Metagenome-Derived Phytase with Optimal Activity at Low pH S
Journal of Microbiology and Biotechnology. 2015. Jun, 25(6): 930-935
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : November 07, 2014
  • Accepted : January 08, 2015
  • Published : June 28, 2015
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About the Authors
Hao Tan
R&D Department, JINDI Agricultural Biotechnology Inc., Chengdu, P.R. China
Xiang Wu
R&D Department, JINDI Agricultural Biotechnology Inc., Chengdu, P.R. China
Liyuan Xie
R&D Department, JINDI Agricultural Biotechnology Inc., Chengdu, P.R. China
Zhongqian Huang
R&D Department, JINDI Agricultural Biotechnology Inc., Chengdu, P.R. China
Bingcheng Gan
R&D Department, JINDI Agricultural Biotechnology Inc., Chengdu, P.R. China
Weihong Peng
R&D Department, JINDI Agricultural Biotechnology Inc., Chengdu, P.R. China
pwh424@163.com

Abstract
A phytase gene was identified in a publicly available metagenome derived from subsurface groundwater, which was deduced to encode for a protein of the histidine acid phosphatase (HAP) family. The nucleotide sequence of the phytase gene was chemically synthesized and cloned, in order to further overexpress the phytase in Escherichia coli. Purified protein of the recombinant phytase demonstrated an activity for phytic acid of 298 ± 17 µmol P/min/mg, at the pH optimum of 2.0 with the temperature of 37℃. Interestingly, the pH optimum of this phytase is much lower in comparison with most HAP phytases known to date. It suggests that the phytase could possess improved adaptability to the low pH condition caused by the gastric acid in livestock and poultry stomachs.
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Acknowledgements
This research was supported in part by grants awarded by The Special Fund for Strategic Emerging Industry Development of Sichuan Province (SC2013510104007), and SAAS Young Scientists Fund (2015QNJJ-015).
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