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Activation of Cryptic hop Genes from Streptomyces peucetius ATCC 27952 Involved in Hopanoid BiosynthesisS
Activation of Cryptic hop Genes from Streptomyces peucetius ATCC 27952 Involved in Hopanoid BiosynthesisS
Journal of Microbiology and Biotechnology. 2015. May, 25(5): 658-661
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : August 25, 2014
  • Accepted : November 18, 2014
  • Published : May 28, 2015
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About the Authors
Gopal Prasad Ghimire
Niranjan Koirala
Jae Kyung Sohng
sohng@sunmoon.ac.kr

Abstract
Genes encoding enzymes with sequence similarity to hopanoids biosynthetic enzymes of other organisms were cloned from the hopanoid ( hop ) gene cluster of Streptomyces peucetius ATCC 27952 and transformed into Streptomyces venezuelae YJ028. The cloned fragments contained four genes, all transcribed in one direction. These genes encode polypeptides that resemble polyprenyl diphosphate synthase ( hopD ), squalene-phytoene synthases ( hopAB ), and squalene-hopene cyclase ( hopE ). These enzymes are sufficient for the formation of the pentacyclic triterpenoid lipid, hopene. The formation of hopene was verified by gas chromatography/mass spectrometry.
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Acknowledgements
This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant#: PJ011119012015), Rural Development Administration, Republic of Korea.
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