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Generation of Minicells from an Endotoxin-Free Gram-Positive Strain Corynebacterium glutamicum
Generation of Minicells from an Endotoxin-Free Gram-Positive Strain Corynebacterium glutamicum
Journal of Microbiology and Biotechnology. 2015. Apr, 25(4): 554-558
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : August 18, 2014
  • Accepted : October 21, 2014
  • Published : April 28, 2015
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About the Authors
Jin-Young Lee
Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea
Hyon E. Choy
Department of Microbiology, Chonnam National University Medical School, Gwangju 500-757, Republic of Korea
Jin-Ho Lee
Department of Food Science and Biotechnology, Kyungsung University, Busan 608-736, Republic of Korea
Geun-Joong Kim
Department of Biological Sciences, College of Natural Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea
gjkim@chonnam.ac.kr

Abstract
Drug delivery systems (DDSs) incorporating bacterial minicells have been evaluated as a very powerful tool in view of biocompatibility. However, limited studies have been carried out on these systems, mainly using minicells from Salmonella sp. and Escherichia coli . Thus, we generated a new minicell-producing strain from an endotoxin-free Corynebacterium glutamicum by the inactivation of genes related to cell division. The two knockout strains, ∆ parA and ∆ ncg l1366, showed distinct abilities to produce minicells. The resulting minicells were purified via sequential antibiotic treatments and centrifugations, which resulted in reproducible yields.
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Acknowledgements
This research was supported by the Basic Science Research Program (Grant No. 20100028752) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology.
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