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Expression of the mexA Gene Requires the DNA Helicase RecG in Pseudomonas aeruginosa PAO1
Expression of the mexA Gene Requires the DNA Helicase RecG in Pseudomonas aeruginosa PAO1
Journal of Microbiology and Biotechnology. 2015. Apr, 25(4): 492-495
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : October 06, 2014
  • Accepted : November 10, 2014
  • Published : April 28, 2015
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About the Authors
Aram Heo
Woojun Park
wpark@korea.ac.kr

Abstract
This study provides evidence that RecG regulates the expression of the OxyR-independent gene mexA in Pseudomonas aeruginosa PAO1. A reduction in mexA expression was observed in the absence of RecG, but not OxyR, by northern blot and quantitative real-time PCR analyses. The canonical palindromic RecG binding sequence was present upstream of the mexA promoter, and bound purified RecG and single strand-binding protein. These data reveal a novel mechanism of OxyR-independent gene transcription by RecG.
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Acknowledgements
This work was supported by the Mid-career Researcher Program through an NRF grant (2014R1A2A2A05007010) funded by the Ministry of Science, ICT, & Future Planning.
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