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Identification and Characterization of the Antifungal Substances of a Novel Streptomyces cavourensis NA4
Identification and Characterization of the Antifungal Substances of a Novel Streptomyces cavourensis NA4
Journal of Microbiology and Biotechnology. 2015. Mar, 25(3): 353-357
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : July 09, 2014
  • Accepted : September 29, 2015
  • Published : March 28, 2015
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About the Authors
Hua-Qi Pan
Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, P.R. China
Su-Ya Yu
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China
Chun-Feng Song
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China
Nan Wang
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China
Hui-Ming Hua
Department of Natural Products Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, P.R. China
hujc@iae.ac.cn
Jiang-Chun Hu
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China
hujc@iae.ac.cn
Shu-Jin Wang
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China

Abstract
A new actinomycete strain NA4 was isolated from a deep-sea sediment collected from the South China Sea and showed promising antifungal activities against soilborne fungal pathogens. It was identified as Streptomyces cavourensis by morphological, physiological, and phylogenetic analyses based on its 16S rRNA gene sequence. The main antifungal components were isolated and identified from the fermentation culture as bafilomycins B1 and C1. These compounds exhibited significant antifungal activities and a broad antifungal spectrum. The results suggest that the Streptomyces cavourensis NA4 and bafilomycins B1 and C1 could be used as potential biocontrol agents for soilborne fungal diseases of plants.
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Acknowledgements
This work was supported by grants from the National High Technology Research and Development Program of China (863 Program) (No. 2012AA092104, 2011AA09070404), National Science Foundation for Young Scientists of China (41006088), and the National Science & Technology Pillar Program (No. 2011BAE06B04). We are grateful to Ms. Wen Li, Ms. Ying Peng, and Mr. Yi Sha of the Shenyang Pharmaceutical University for recording NMR and MS spectra..
References
Anees M , Edel-Hermann V , Steinberg C 2010 Build up of patches caused byRhizoctonia solani. Soil Biol. Biochem. 42 1661 - 1672    DOI : 10.1016/j.soilbio.2010.05.013
Carr G , Williams DE , Diìaz-Marrero AR , Patrick BO , Bottriell H , Balgi AD 2009 Bafilomycins produced in culture byStreptomycesspp. isolated from marine habitats are potent inhibitors of autophagy. J. Nat. Prod. 73 422 - 427    DOI : 10.1021/np900632r
Chen L , Wang N , Wang X , Hu J , Wang S 2010 Characterization of two anti-fungal lipopeptides produced byBacillus amyloliquefaciensSH-B10. Bioresour. Technol. 101 8822 - 8827    DOI : 10.1016/j.biortech.2010.06.054
Frändberg E , Petersson C , Lundgren LN , Schnürer J 2000 Streptomyces halstediiK122 produces the antifungal compounds bafilomycin B1 and C1. Can. J. Microbiol. 46 753 - 758    DOI : 10.1139/w00-050
Fravel D , Olivain C , Alabouvette C 2003 Fusarium oxysporumand its biocontrol. New Phytol. 157 493 - 502    DOI : 10.1046/j.1469-8137.2003.00700.x
Kim B , Hwang B 2007 Microbial fungicides in the control of plant diseases. J. Phytopathol. 155 641 - 653    DOI : 10.1111/j.1439-0434.2007.01314.x
Li J , Lu C , Shen Y 2010 Macrolides of the bafilomycin family produced byStreptomycessp. CS. J. Antibiot. 63 595 - 599    DOI : 10.1038/ja.2010.95
Peng JN , Shen XY , El Sayed KA , Dunbar DC , Perry TL , Wilkins SP , Hamann MT 2003 Marine natural produc ts as prototype agrochemical agents. J. Agric. Food Chem. 51 2246 - 2252    DOI : 10.1021/jf0207880
Poli A , Gilardi G , Spadaro D , Gullino M , Garibaldi A 2012 Molecular characterization ofFusarium oxysporumf.sp.cichoriipathogenic on chicory (Cichorium intybus). Phytoparasitica 40 383 - 391    DOI : 10.1007/s12600-012-0239-z
Shanzhao J , Ling L , Zhaopu L , Xiaohua L , Hongbo S , Jiayao C 2013 Characterization of marinePseudomonasspp. antagonist towards three tuber-rotting fungi from Jerusalem artichoke, a new industrial crop. Ind. Crops Prod. 43 556 - 561    DOI : 10.1016/j.indcrop.2012.07.038
Silva L , Taketani R , Melo I , Goodfellow M , Zucchi T 2013 Streptomyces araujoniaesp. nov.: an actinomycete isolated from a potato tubercle. Antonie Van Leeuwenhoek 103 1235 - 1244    DOI : 10.1007/s10482-013-9901-9
Tareq FS , Kim JH , Lee MA , Lee HS , Lee JS , Lee YJ , Shin HJ 2013 Antimicrobial gageomacrolactins characterized from the fermentation of the marine-derived bacteriumBacillus subtilisunder optimum growth conditions. J. Agric. Food Chem. 61 3428 - 3434    DOI : 10.1021/jf4009229
Uyeda M , Kondo K , Ito A , Yokomizo K , Kido Y 1995 A new antiherpetic agent produced byStreptomycessp. strain no. 758. J. Antibiot. 48 1234 -    DOI : 10.7164/antibiotics.48.1234
Wang X , Radwan MM , Taraìwneh AH , Gao J , Wedge DE , Rosa LH 2013 Antifungal activity against plant pathogens of metabolites from the endophytic fungusCladosporium cladosporioides. J. Agric. Food Chem. 61 4551 - 4555    DOI : 10.1021/jf400212y
Werner G , Hagenmaier H , Drautz H , Baumgartner A , Zahner H 1984 Metabolic products of microorganisms. 224. Bafilomycins, a new group of macrolide antibiotics. Production, isolation, chemical structure and biological activity. J. Antibiot. 37 110 - 117    DOI : 10.7164/antibiotics.37.110
Xie L , Jiang S , Zhu H , Sun W , Ouyang Y , Dai S , Li X 2008 Potential inhibitors againstSclerotinia sclerotiorum, produced by the fungusMyrotheciumsp. assoc iated with the marine spongeAxinellasp. Eur. J. Plant Pathol. 122 571 - 578    DOI : 10.1007/s10658-008-9326-x
Xu WP , Zhang DJ , Si CC , Tao LM 2013 Antifungal macrolides fromStreptomyces cavourensisYY01-17. Chem. Nat. Compounds 49 988 - 989    DOI : 10.1007/s10600-013-0804-9
Yoon MY , Cha B , Kim JC 2013 Recent trends in studies on botanical fungicides in agriculture. Plant Pathol. J. 29 1 - 9    DOI : 10.5423/PPJ.RW.05.2012.0072
Zhang DJ , Wei G , Wang Y , Si CC , Tian L , Tao LM , Li YG 2011 Bafilomycin K, a new antifungal macrolide fromStreptomyces flavotriciniY12-26. J. Antibiot. 64 391 - 393    DOI : 10.1038/ja.2011.12