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VvpM Induces Human Cell Death via Multifarious Modes Including Necroptosis and Autophagy
VvpM Induces Human Cell Death via Multifarious Modes Including Necroptosis and Autophagy
Journal of Microbiology and Biotechnology. 2015. Feb, 25(2): 302-306
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : January 06, 2015
  • Accepted : January 14, 2015
  • Published : February 28, 2015
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About the Authors
Mi-Ae Lee
Department of Life Science, Sogang University, Seoul 121-742, Republic of Korea
Jeong-A Kim
Department of Life Science, Sogang University, Seoul 121-742, Republic of Korea
Mee-Young Shin
Department of Environmental Medical Biology, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea
Jeong K. Lee
Department of Life Science, Sogang University, Seoul 121-742, Republic of Korea
Soon-Jung Park
Department of Environmental Medical Biology, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea
Kyu-Ho Lee
Department of Life Science, Sogang University, Seoul 121-742, Republic of Korea
kyuholee@sogang.ac.kr

Abstract
VvpM, one of the extracellular metalloproteases produced by Vibrio vulnificus , induces apoptotic cell death via a pathway consisting of ERK activation, cytochrome c release, and activation of caspases-9 and -3. VvpM-treated cells also showed necrotic cell death as stained by propidium iodide (PI). The percentage of PI-stained cells was decreased by pretreatment with Necrostatin-1, indicating that VvpM-mediated cell death occurs through necroptosis. The appearance of autophagic vesicles and lipidated form of light-chain-3B in rVvpM-treated cells suggests an involvement of autophagy in this process. Therefore, the multifarious action of VvpM might be one of the factors responsible for V. vulnificus pathogenesis.
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Acknowledgements
This work was supported by a grant (14162MFDS972) from the Ministry of Food and Drug Safety, and by the Pioneer Research Center Program through NRF funded by the Ministry of Science, ICT & Future Planning (2013M3C1A3064325).
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