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Effect of pH on the Formation of Lysosome-Alginate Beads for Antimicrobial Activity
Effect of pH on the Formation of Lysosome-Alginate Beads for Antimicrobial Activity
Journal of Microbiology and Biotechnology. 2015. Feb, 25(2): 234-237
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : October 13, 2014
  • Accepted : November 19, 2014
  • Published : February 28, 2015
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About the Authors
Hyun Jung Park
Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea
Jiho Min
Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea
Joo-Myung Ahn
Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea
Sung-Jin Cho
Department of Biology, Chungbuk National University, Cheongju 361-763, Republic of Korea
Ji-Young Ahn
Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea
kyh@chungbuk.ac.kr
Yang-Hoon Kim
Department of Microbiology, Chungbuk National University, Cheongju 361-763, Republic of Korea
kyh@chungbuk.ac.kr

Abstract
In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were generated, and the level of lysosome release was investigated by using antimicrobial tests. At low pH, lysosomes were not released from the lysosome-alginate beads; however, at neutral pH, similar to the pH in the intestine, lysosome release was confirmed, as determined by a high antimicrobial activity. This study shows the potential of such an ODDS for the in vivo treatment of infection with pathogens.
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Acknowledgements
This work was supported by a grant from the NextGeneration BioGreen 21 Program (CABX, No. PJ010001), Rural Development Administration, Republic of Korea. The authors are grateful for their support.
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