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Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase
Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase
Journal of Microbiology and Biotechnology. 2015. Dec, 25(12): 2007-2010
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : July 22, 2015
  • Accepted : September 15, 2015
  • Published : December 28, 2015
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About the Authors
Jin-Beom Cho
Department of Bio and Nanochemistry, Kookmin University, Seoul 02707, Republic of Korea
Jin-Moo Lee
Department of Bio and Nanochemistry, Kookmin University, Seoul 02707, Republic of Korea
Hee-Chul Ahn
Department of Pharmacy, Dongguk University-Seoul, Goyang 10326, Republic of Korea
Yong-Joo Jeong
Department of Bio and Nanochemistry, Kookmin University, Seoul 02707, Republic of Korea

Abstract
A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC 50 values of 8.66 ± 0.26 μM and 41.6 ± 2.3 μM, respectively. Moreover, we observed that the compound did not show cytotoxicity up to 80 μM concentration. Our results suggest that the compound might serve as a SARS coronavirus inhibitor.
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( A ) ATP hydrolysis activity. ( B ) dsDNA unwinding activity.
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Acknowledgements
This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A2A10061193).
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