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Building Triketide α-Pyrone-Producing Yeast Platform Using Heterologous Expression of Sporopollenin Biosynthetic Genes
Building Triketide α-Pyrone-Producing Yeast Platform Using Heterologous Expression of Sporopollenin Biosynthetic Genes
Journal of Microbiology and Biotechnology. 2015. Nov, 25(11): 1796-1800
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : June 08, 2015
  • Accepted : July 24, 2015
  • Published : November 28, 2015
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Sung Soo Kim
sskim227@gmail.com

Abstract
Sporopollenin is a poorly characterized mixed aliphatic and aromatic polymer with ester and ether linkages. Recent studies have reported that α-pyrone polyketide compounds generated by Arabidopsis thaliana , polyketide synthase A (PKSA) and tetraketide α-pyrone reductase 1 (TKPR1), are previously unknown sporopollenin precursors. Here, the yeast Saccharomyces cerevisiae was introduced to test potential sporopollenin biosynthetic pathways in vivo . A PKSA/TKPR1 dual expressor was generated and various chain-length alkyl α-pyrones were identified by GC-MS. The growth rate of the strain containing PKSA/TKPR1 appeared normal. These results indicate that PKSA/TKPR1-expressing yeast would be a starting platform to investigate in vivo sporopollenin metabolism.
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Acknowledgements
This study was supported by Samsung Advanced Institute of Technology in the Republic of Korea. I would like to thank Prof. Carl J. Douglas at the University of British Columbia, Canada for comments on the manuscript.
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