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Specific and Sensitive Detection of the Pear Scab Fungus Venturia nashicola by SYBR Green Real-Time PCR
Specific and Sensitive Detection of the Pear Scab Fungus Venturia nashicola by SYBR Green Real-Time PCR
Journal of Microbiology and Biotechnology. 2015. Nov, 25(11): 1782-1786
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : July 30, 2015
  • Accepted : September 01, 2015
  • Published : November 28, 2015
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About the Authors
Yeo Hong Yun
Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 330-714, Republic of Korea
Seong Kwon Yoon
Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 330-714, Republic of Korea
Jae Sung Jung
Department of Biology, Sunchon National University, Suncheon 540-950, Republic of Korea
Seong Hwan Kim
Department of Microbiology and Institute of Biodiversity, Dankook University, Cheonan 330-714, Republic of Korea
piceae@dankook.ac.kr

Abstract
A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola , a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the target gene from the genomic DNA of V. nashicola . No amplicon was produced from the genomic DNA of other Venturia spp. and reference fungal species tested. With the high detection limit of 10 fg DNA content, our real-time method could be used for the quarantine inspection and field monitoring of V. nashicola .
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Acknowledgements
This study was supported by the Rural Development Administration (PJ007883042014).
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