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Cohesion Establishment Factors Stimulate Endonuclease Activity of hFen1 Independently and Cooperatively
Cohesion Establishment Factors Stimulate Endonuclease Activity of hFen1 Independently and Cooperatively
Journal of Microbiology and Biotechnology. 2015. Oct, 25(10): 1768-1771
Copyright © 2015, The Korean Society For Microbiology And Biotechnology
  • Received : March 25, 2015
  • Accepted : May 28, 2015
  • Published : October 28, 2015
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About the Authors
Do-Hyung Kim
Bio Research Team, LG Household & Health Care Research Park, Daejeon 305-343, Republic of Korea
Jeong-Hoon Kim
Epigenomics Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Republic of Korea
Byoung Chul Park
Functional Proteomics Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Republic of Korea
Sayeon Cho
College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea
sgpark@kribb.re.kr
Sung Goo Park
Functional Proteomics Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Republic of Korea
sgpark@kribb.re.kr

Abstract
Human Fen1 protein (hFen1) plays an important role in Okazaki fragment processing by cleaving the flap structure at the junction between single-stranded (ss) DNA and doublestranded (ds) DNA, an intermediate formed during Okazaki fragment processing, resulting in ligatable nicked dsDNA. It was reported that hChlR1, a member of the cohesion establishment factor family, stimulates hFen1 nuclease activity regardless of its ATPase activity. In this study, we found that cohesion establishment factors cooperatively stimulate endonuclease activity of hFen1 in in vivo mimic condition, including replication protein-A-coated DNA and high salt. Our findings are helpful to explain how a DNA replication machinery larger than the cohesion complex goes through the cohesin ring structure on DNA during S phase in the cell cycle.
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Acknowledgements
We thank Dr. Jerard Hurwitz for providing the proteins. We are also grateful to Drs. Kwang-Hee Bae and Sang-Chul Lee for helpful advice and careful reading of the manuscript. This work was supported by a grant from the National Research Foundation (NRF-2011-0028172).
References
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