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Enzymatic Synthesis of 2-Phenoxyethanol Galactoside by Whole Cells of <TEX>${\beta}$</TEX>-Galactosidase-Containing Escherichia coli
Enzymatic Synthesis of 2-Phenoxyethanol Galactoside by Whole Cells of ${\beta}$-Galactosidase-Containing Escherichia coli
Journal of Microbiology and Biotechnology. 2014. Sep, 24(9): 1254-1259
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : April 02, 2014
  • Accepted : May 19, 2014
  • Published : September 28, 2014
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About the Authors
Hyang-Yeol Lee
Kyung-Hwan Jung
khjung@cjnu.ac.kr

Abstract
We investigated whether β-galactosidase (β-gal)-containing Escherichia coli cells could transfer a galactose to 2-phenoxyethanol, resulting in 2-phenoxyethanol galactoside (PE-Gal). PE-Gal was confirmed by liquid chromatography-mass spectrometry. In addition, we also confirmed that a galactose molecule was covalently bonded with PE during thin-layer chromatography analysis of the β-gal hydrolysate of PE-Gal. The yield for PE-Gal synthesis was about 37.5% (weight basis), which was about 7–8 times greater than that of a previous report. In addition, the concentration of β-gal (0.96 U/ml) used in this PE-Gal synthesis was about 20 times less than that in a previous report.
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Acknowledgements
The author thanks Ms. Ju-Hye Yeon for their technical assistance.
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