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Bacillus licheniformis Isolated from Korean Traditional Food Sources Enhances the Resistance of Caenorhabditis elegans to Infection by Staphylococcus aureus
Bacillus licheniformis Isolated from Korean Traditional Food Sources Enhances the Resistance of Caenorhabditis elegans to Infection by Staphylococcus aureus
Journal of Microbiology and Biotechnology. 2014. Aug, 24(8): 1105-1108
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : June 03, 2014
  • Accepted : June 07, 2014
  • Published : August 30, 2014
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About the Authors
Hyun Sun Yun
BK21 Plus Graduate Program, Department of Animal Science, Chonbuk National University, Jeonju 561-756, Republic of Korea
Ju Hee Heo
Microbial Institute for Fermentation Industry, Sunchang, Jeonbuk 595-804, Republic of Korea
Seok Jun Son
BK21 Plus Graduate Program, Department of Animal Science, Chonbuk National University, Jeonju 561-756, Republic of Korea
Mi Ri Park
BK21 Plus Graduate Program, Department of Animal Science, Chonbuk National University, Jeonju 561-756, Republic of Korea
Sangnam Oh
BK21 Plus Graduate Program, Department of Animal Science, Chonbuk National University, Jeonju 561-756, Republic of Korea
Min-Ho Song
Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea
Jong Nam Kim
Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea
Gwang-Woong Go
Department of Food and Nutrition, Kookmin University, Seoul 136-703, Republic of Korea
Ho-Seong Cho
Bio-safety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Republic of Korea
Nag-Jin Choi
BK21 Plus Graduate Program, Department of Animal Science, Chonbuk National University, Jeonju 561-756, Republic of Korea
Seung-Wha Jo
Microbial Institute for Fermentation Industry, Sunchang, Jeonbuk 595-804, Republic of Korea
Do-Youn Jeong
Microbial Institute for Fermentation Industry, Sunchang, Jeonbuk 595-804, Republic of Korea
Younghoon Kim
BK21 Plus Graduate Program, Department of Animal Science, Chonbuk National University, Jeonju 561-756, Republic of Korea
ykeys2584@jbnu.ac.kr

Abstract
We investigated whether Bacillus spp., newly isolated from Korean traditional food resources, influence the resistance of hosts to foodborne pathogens, by using Caenorhabditis elegans as a surrogate host model. Initially, we selected 20 Bacillus spp. that possess antimicrobial activity against various foodborne pathogens, including Staphylococcus aureus . Among the selected strains, six strains of Bacillus spp. used in preconditioning significantly prolonged the survival of nematodes exposed to S. aureus . Based on 16S rRNA gene sequencing, all six strains were identified as B. licheniformis . Our findings suggest that preconditioning with B. licheniformis may modulate the host defense response against S. aureus .
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Acknowledgements
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2014R1A1A1006872) to Y.K., and the Ministry for Food, Agriculture, Forestry, and Fisheries of Republic of Korea (313036-03-1-HD020) and the Ministry of Trade, Industry, and Energy (10045726).
References
Begley M , Cotter PD , Hill C , Ross RP 2009 Identification of a novel two-peptide lantibiotic, lichenicidin, following rational genome mining for LanM proteins. Appl. Environ. Microbiol. 75 5451 - 5460    DOI : 10.1128/AEM.00730-09
Brenner S 1974 The genetics of Caenorhabditis elegans. Genetics 77 71 - 94
Deng W , Dong XF , Tong JM , Zhang Q 2012 The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology, and intestinal mucosal immunity in laying hens. Poult. Sci. 91 575 - 582    DOI : 10.3382/ps.2010-01293
Dobson A , Cotter PD , Ross RP , Hill C 2012 Bacteriocin production: a probiotic trait? Appl. Environ. Microbiol. 78 1 - 6    DOI : 10.1128/AEM.05576-11
Guo Y , Yu Z , Xie J , Zhang R 2012 Identification of a new Bacillus licheniformis strain producing a bacteriocin-like substance. J. Microbiol. 50 452 - 458    DOI : 10.1007/s12275-012-2051-3
Huang JM , La Ragione RM , Nunez A , Cutting SM 2008 Immunostimulatory activity of Bacillus spores. FEMS Immunol. Med. Microbiol. 53 195 - 203    DOI : 10.1111/j.1574-695X.2008.00415.x
Irazoqui JE , Urbach JM , Ausubel FM 2010 Evolution of host innate defence: insights from Caenorhabditis elegans and primitive invertebrates. Nat. Rev. Immunol. 10 47 - 58    DOI : 10.1038/nri2689
Kim NY , Song EJ , Kwon DY , Kim HP , Heo MY 2008 Antioxidant and antigenotoxic activities of Korean fermented soybean. Food Chem. Toxicol. 46 1184 - 1189    DOI : 10.1016/j.fct.2007.12.003
Kim Y , Mylonakis E 2012 Caenorhabditis elegans immune conditioning with the probiotic bacterium Lactobacillus acidophilus strain NCFM enhances gram-positive immune responses. Infect. Immun. 80 2500 - 2508    DOI : 10.1128/IAI.06350-11
Kim Y , Yun S , Jeong D , Hahn K , Uhm T 2010 Isolation of Bacillus licheniformis producing antimicrobial agents against Bacillus cereus and its properties. Kor. J. Microbiol. 46 270 - 277
Knap I , Lund B , Kehlet AB , Hofacre C , Mathis G 2010 Bacillus licheniformis prevents necrotic enteritis in broiler chickens. Avian Dis. 54 931 - 935    DOI : 10.1637/9106-101509-ResNote.1
Millette M , Dupont C , Archambault D , Lacroix M 2007 Partial characterization of bacteriocins produced by human Lactococcus lactis and Pediococccus acidilactici isolates. J. Appl. Microbiol. 102 274 - 282    DOI : 10.1111/j.1365-2672.2006.03044.x
Ranjit Kumar N , Raman R , Jadhao S , Brahmchari R , Kumar K , Dash G 2013 Effect of dietary supplementation of Bacillus licheniformis on gut microbiota, growth and immune response in giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879). Aquacult. Int. 21 387 - 403    DOI : 10.1007/s10499-012-9567-8
Sorokulova IB , Pinchuk IV , Denayrolles M , Osipova IG , Huang JM , Cutting SM , Urdaci MC 2008 The safety of two Bacillus probiotic strains for human use. Dig. Dis. Sci. 53 954 - 963    DOI : 10.1007/s10620-007-9959-1
Yoo S , Kang S , Noh Y 2000 Quality properties on soy bean pastes made with microorganisms isolated from traditional soy bean pastes. Kor. J. Food Sci. Technol. 32 1266 - 1270