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Characterization of CTX-M-14- and CTX-M-15-Producing Escherichia coli and Klebsiella pneumoniae Isolates from Urine Specimens in a Tertiary-Care Hospital
Characterization of CTX-M-14- and CTX-M-15-Producing Escherichia coli and Klebsiella pneumoniae Isolates from Urine Specimens in a Tertiary-Care Hospital
Journal of Microbiology and Biotechnology. 2014. Jun, 24(6): 765-770
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : June 13, 2013
  • Accepted : March 13, 2014
  • Published : June 28, 2014
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About the Authors
Semi Kim
Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon 301-721, Republic of Korea
Ji Youn Sung
Department of Biomedical Laboratory Science, Far East University, Eumseong 369-700, Republic of Korea
Hye Hyun Cho
Department of Biomedical Laboratory Science, Jeonju Kijeon College, Jeonju 560-701, Republic of Korea
Kye Chul Kwon
Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon 301-721, Republic of Korea
Sun Hoe Koo
Department of Laboratory Medicine, College of Medicine, Chungnam National University, Daejeon 301-721, Republic of Korea
shkoo@cnu.ac.kr

Abstract
This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and 24 (77.4%) K. pneumoniae isolates containing bla CTX-M , bla CTX-M-14 and bla CTX-M-15 were detected in 23 and 19 E. coli isolates, respectively, and in 7 and 17 K. pneumoniae isolates, respectively. CTX-M producers of urinary E. coli and K. pneumoniae were resistant to multiple antibiotics and contained other antimicrobial resistance genes. CTX-M-15 producers contained more antimicrobial resistance genes than did CTX-M-14 producers.
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