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Construction of Artificial Biosynthetic Pathways for Resveratrol Glucoside Derivatives
Construction of Artificial Biosynthetic Pathways for Resveratrol Glucoside Derivatives
Journal of Microbiology and Biotechnology. 2014. May, 24(5): 614-618
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : January 16, 2014
  • Accepted : February 20, 2014
  • Published : May 28, 2014
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About the Authors
Oksik Choi
Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang-eup 363-883, Republic of Korea
Jae Kyoung Lee
Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang-eup 363-883, Republic of Korea
Sun-Young Kang
Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang-eup 363-883, Republic of Korea
Ramesh Prasad Pandey
Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, Asan-si 336-708, Republic of Korea
Jae-Kyung Sohng
Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, Asan-si 336-708, Republic of Korea
Jong Seog Ahn
Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang-eup 363-883, Republic of Korea
Young-Soo Hong
Chemical Biology Research Center, Korea Research Institute of Bioscience and Biotechnology, Ochang-eup 363-883, Republic of Korea
hongsoo@kribb.re.kr

Abstract
Resveratrol, which is a polyphenolic antioxidant, is dose-dependent when used to provide health benefits, to enhance stress resistance, and to extend lifespans. However, even though resveratrol has therapeutic benefits, its clinical therapeutic effect is limited owing to its low oral bioavailability. An Escherichia coli system was developed that contains an artificial biosynthetic pathway that produces resveratrol glucoside derivatives, such as resveratrol-3- O -glucoside (piceid) and resveratrol-4’- O -glucoside (resveratroloside), from simple carbon sources. This artificial biosynthetic pathway contains a glycosyltransferase addition (YjiC from Bacillus ) with resveratrol biosynthetic genes. The produced glucoside compounds were verified through the presence of a product peak(s) and also through LC/MS analyses. The strategy used in this research demonstrates the first harnessing of E. coli for de novo synthesis of resveratrol glucoside derivatives from a simple sugar medium.
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Acknowledgements
This work was supported in part by a grant from the KRIBB Research Initiative Program and Basic Science Research program (2012–0001421) funded by the NRF and by the Next-Generation BioGreen 21 Program (SSAC, PJ009549022013) funded by the RDA, Republic of Korea.
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