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Development of a Novel Immunochromatographic Assay for Rapid Detection of VanA Ligase-Producing Vancomycin-Resistant Enterococci
Development of a Novel Immunochromatographic Assay for Rapid Detection of VanA Ligase-Producing Vancomycin-Resistant Enterococci
Journal of Microbiology and Biotechnology. 2014. Mar, 24(3): 427-430
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : July 11, 2013
  • Accepted : December 11, 2013
  • Published : March 28, 2014
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About the Authors
Gil Yong Ji
Dinona Co, Iksan 570-912, Republic of Korea
Hyung Geun Song
Dinona Co, Iksan 570-912, Republic of Korea
Bo Ra Son
Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju 361-763, Republic of Korea
Seung Bok Hong
Department of Clinical Laboratory Science, Chungbuk Health and Science University, Cheongwon 363-794, Republic of Korea
Jong Wan Kim
Department of Laboratory Medicine, Dankook University College of Medicine, Cheonan 330-714, Republic of Korea
Kyeong Seob Shin
Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju 361-763, Republic of Korea
ksshin@chungbuk.ac.kr

Abstract
We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detection limit of the kit was 6.3 × 10 6 CFU per test. Of 127 vancomycin-resistant microorganisms, 100 vanA VRE were positive in the VanA ligase ICA, and 27 non- vanA vancomycin-resistant isolates were negative. These results were consistent with those of the PCR analyses. Thus, our ICA is a reliable and easy-to-use immunological assay for detecting VanA-producing VRE in clinical laboratories.
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Acknowledgements
We thank Jimyung Kim (Chungnam National University Hospital) and Ja Yung Kim (Catholic University Daejeon St. Mary’s Hospital) for providing clinical isolates of vancomycin-resistant enterococci.
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