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Enzymatic Production of 15-Hydroxyeicosatetraenoic Acid from Arachidonic Acid by Using Soybean Lipoxygenase
Enzymatic Production of 15-Hydroxyeicosatetraenoic Acid from Arachidonic Acid by Using Soybean Lipoxygenase
Journal of Microbiology and Biotechnology. 2014. Mar, 24(3): 359-362
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : October 15, 2013
  • Accepted : November 29, 2013
  • Published : March 28, 2014
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About the Authors
Baek-Joong Kim
Kyung-Chul Shin
Deok-Kun Oh
deokkun@konkuk.ac.kr

Abstract
15-Hydroxyeicosatetraenoic acid (HETE), as a mammalian biologically active metabolite, has anticarcinogenic effect. The conditions of producing 15-HETE from arachidonic acid by using soybean lipoxygenase were optimal at pH 8.5 and 20℃ with 9 g/l arachidonic acid, 54.4 U/ml soybean lipoxygenase, and 4% methanol. Under these optimized conditions, the enzyme produced 9.5 g/l 15-HETE after 25 min, with a molar conversion yield of 99% and a productivity of 22.8 g l -1 h -1 . To the best of our knowledge, this is the first biotechnological production of 15-HETE.
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Acknowledgements
This study was supported by a grant from the Bioindustry Technology Development Program, Ministry for Food, Agriculture, Forestry and Fisheries (No. 112002-3), and a grant from the Korea Healthcare Technology R&D Project, Ministry for Health and Welfare, Republic of Korea (No. 2012-009).
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