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Purification and Characterization of <TEX>${\alpha}$</TEX>-Neoagarooligosaccharide Hydrolase from Cellvibrio sp. OA-2007
Purification and Characterization of ${\alpha}$-Neoagarooligosaccharide Hydrolase from Cellvibrio sp. OA-2007
Osamu Ariga , Naoki Okamoto , Naomi Harimoto , Kiyohiko Nakasaki
Journal of Microbiology and Biotechnology. 2014. Jan, 24(1): 48-51
DOI : http://dx.doi.org/10.4014/jmb.1307.07018
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : July 10, 2013
  • Accepted : September 27, 2013
  • Published : January 28, 2014
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Osamu Ariga
Naoki Okamoto
Naomi Harimoto
Kiyohiko Nakasaki
About the Authors
Osamu Ariga
School of Environmental Science and Engineering, Kochi University of Technology, 782-8502, Kochi, Japan
ariga.osamu@kochi-tech.ac.jp
Naoki Okamoto
School of Environmental Science and Engineering, Kochi University of Technology, 782-8502, Kochi, Japan
Naomi Harimoto
School of Environmental Science and Engineering, Kochi University of Technology, 782-8502, Kochi, Japan
Kiyohiko Nakasaki
International Development Engineering, Graduate School of Engineering, Tokyo Institute of Technology, Tokyo 152-8550, Japan
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Abstract
Keywords

Abstract
α-Neoagarooligosaccharide (α-NAOS) hydrolase was purified from Cellvibrio sp. OA-2007 by using chromatographic techniques after hydroxyapatite adsorption. The molecular masses of α-NAOS hydrolase estimated using SDS-PAGE and gel filtration chromatography were 40 and 93 kDa, respectively, and the optimal temperature and pH for the enzyme activity were 32℃ and 7.0-7.2. α-NAOS hydrolase lost 43% of its original activity when incubated at 35℃ for 30 min. The enzyme hydrolyzed neoagarobiose, neoagarotetraose, and neoagarohexaose to galactose, agarotriose, and agaropentaose, respectively, and produced 3,6-anhydro-L-galactose concomitantly; however, it did not degrade agarose.
Keywords
Cellvibrio sp. ;  α-NAOS hydrolase ;  neoagarooligosaccharide
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1.
Ariga O , Inoue T , Kubo H , Minami K , Nakamura M , Iwai M 2012 Cloning of agarase gene from non-marine agarolytic bacterium Cellvibrio sp. J. Microbiol. Biotechnol. 22 1237 - 1244    DOI : 10.4014/jmb.1202.02020
2.
Chen HM , Yan XJ 2005 Antioxidant activities of agarooligosaccharides with different degrees of polymerization in cell-based system. Biochim. Biophys. Acta 1722 103 - 111    DOI : 10.1016/j.bbagen.2004.11.016
3.
Chen H , Yan X , Zhu P , Lin J 2006 Antioxidant activity and hepatoprotective potential of agaro-oligosaccharides in vitro and in vivo. Nutr. J. 31 1 - 12
4.
Enoki T , Okuda S , Kudo Y , Takashima F , Sagawa H , Kato I 2010 Oligosaccharides from agar inhibit pro-inflammatory mediator release by inducing heme oxygenase 1. Biosci. Biotechnol. Biochem. 74 766 - 770    DOI : 10.1271/bbb.90803
5.
Enoki T , Tominaga T , Takashima F , Ohnogi H , Sagawa H , Kato I 2012 Anti-tumor-promoting activities of agarooligosaccharides on two-stage mouse skin carcinogenesis. Biol. Pharm. Bull. 35 1145 - 1149    DOI : 10.1248/bpb.b12-00188
6.
Hashimoto W , Kawai S , Murata K 2010 Bacterial supersystem for alginate import/metabolism and its environmental and bioenergy applications. Bioeng. Bugs 1 97 - 109    DOI : 10.4161/bbug.1.2.10322
7.
Hassairi I , Amar RB , Nonus M , Gupta BB 2001 Production and separation of α-agarase from Altermonas agarlyticus strain GJ1B. Biores. Technol. 79 47 - 51    DOI : 10.1016/S0960-8524(01)00037-2
8.
Hatada Y , Ohta Y , Horikoshi K 2006 Hyperproduction and application of alpha-agarase to enzymatic enhancement of antioxidant activity of porphyran. J. Agric. Food Chem. 54 9895 - 9900    DOI : 10.1021/jf0613684
9.
Kim HT , Yun EJ , Wang D , Chung JH , Choi IG , Kim KH 2013 High temperature and low acid pretreatment and agarase treatment of agarose for the production of sugar and ethanol from red seaweed biomass. Bioresour. Technol. 136 582 - 587    DOI : 10.1016/j.biortech.2013.03.038
10.
Kobayashi R , Takisada M , Suzuki T , Kirimura K , Usami S 61 Neoagarobiose as a novel moisturizer with whitening effect. Biosci. Biotechnol. Biochem. 1997 162 - 163
11.
Lee JY , Li P , Lee J , Ryu HJ , Oh KK 2013 Ethanol production from Saccharina japonica using an optimized extremely low acid pretreatment followed by simultaneous saccharification and fermentation. Bioresour. Technol. 127 119 - 125    DOI : 10.1016/j.biortech.2012.09.122
12.
Ohta Y , Hatada Y , Miyazaki M , Nogi Y , Ito S , Horikoshi K 2005 Purification and characterization of a novel alphaagarase from a Thalassomonas sp. Curr. Microbiol. 50 212 - 216    DOI : 10.1007/s00284-004-4435-z
13.
Philippe P , Richard C , Rochas C , Kloareg B 1993 Purification and characterization of the α-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B. Eur. J. Biochem. 214 599 - 607    DOI : 10.1111/j.1432-1033.1993.tb17959.x
14.
Seok JH , Kim HS , Hatada Y , Nam SW , Kim YH 2012 Construction of an expression system for the secretory production of recombinant α-agarase in yeast. Biotechnol. Lett. 34 1041 - 1049    DOI : 10.1007/s10529-012-0864-0
15.
Sugano Y , Kodama H , Terada I , Yamazaki Y , Noma M 1994 Purification and characterization of a novel enzyme, alpha-neoagarooligosaccharide hydrolase (alpha-NAOS hydrolase), from a marine bacterium, Vibrio sp. strain JT0107. J. Bacteriol. 176 6812 - 6818
16.
Suzuki H , Sawai Y , Suzuki T , Kawai K 2002 Purification and characterization of an extracellular alpha-neoagarooligosaccharide hydrolase from Bacillus sp. MK03. J. Biosci. Bioeng. 93 456 - 463
17.
Wargacki AJ , Leonard E , Win MN , Regitsky DD , Santos CN , Kim PB 2012 An engineered microbial platform for direct biofuel production from brown macroalgae. Science. 335 308 - 313    DOI : 10.1126/science.1214547
Citing 'Purification and Characterization of ${\alpha}$-Neoagarooligosaccharide Hydrolase from Cellvibrio sp. OA-2007 '
@article{ E1MBA4_2014_v24n1_48} ,title={Purification and Characterization of ${\alpha}$-Neoagarooligosaccharide Hydrolase from Cellvibrio sp. OA-2007} ,volume={1} , url={http://dx.doi.org/10.4014/jmb.1307.07018}, DOI={10.4014/jmb.1307.07018} , number= {1} , journal={Journal of Microbiology and Biotechnology} , publisher={The Korean Society for Applied Microbiology and Biotechnology} , author={Ariga, Osamu and Okamoto, Naoki and Harimoto, Naomi and Nakasaki, Kiyohiko} , year={2014} , month={Jan}