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Purification and Characterization of α-Neoagarooligosaccharide Hydrolase from Cellvibrio sp. OA-2007
Purification and Characterization of α-Neoagarooligosaccharide Hydrolase from Cellvibrio sp. OA-2007
Journal of Microbiology and Biotechnology. 2014. Jan, 24(1): 48-51
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : July 10, 2013
  • Accepted : September 27, 2013
  • Published : January 30, 2014
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About the Authors
Osamu Ariga
School of Environmental Science and Engineering, Kochi University of Technology, 782-8502, Kochi, Japan
ariga.osamu@kochi-tech.ac.jp
Naoki Okamoto
School of Environmental Science and Engineering, Kochi University of Technology, 782-8502, Kochi, Japan
Naomi Harimoto
School of Environmental Science and Engineering, Kochi University of Technology, 782-8502, Kochi, Japan
Kiyohiko Nakasaki
International Development Engineering, Graduate School of Engineering, Tokyo Institute of Technology, Tokyo 152-8550, Japan

Abstract
α-Neoagarooligosaccharide (α-NAOS) hydrolase was purified from Cellvibrio sp. OA-2007 by using chromatographic techniques after hydroxyapatite adsorption. The molecular masses of α-NAOS hydrolase estimated using SDS-PAGE and gel filtration chromatography were 40 and 93 kDa, respectively, and the optimal temperature and pH for the enzyme activity were 32℃ and 7.0-7.2. α-NAOS hydrolase lost 43% of its original activity when incubated at 35℃ for 30 min. The enzyme hydrolyzed neoagarobiose, neoagarotetraose, and neoagarohexaose to galactose, agarotriose, and agaropentaose, respectively, and produced 3,6-anhydro-L-galactose concomitantly; however, it did not degrade agarose.
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