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Identification of Novel Phytase Genes from an Agricultural Soil-Derived MetagenomeS
Identification of Novel Phytase Genes from an Agricultural Soil-Derived MetagenomeS
Journal of Microbiology and Biotechnology. 2014. Jan, 24(1): 113-118
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : July 08, 2013
  • Accepted : October 10, 2013
  • Published : January 30, 2014
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About the Authors
Hao Tan
BIOMERIT Research Centre, Microbiology Department, University College Cork, National University of Ireland, Cork, Ireland
Marlies J.Mooij
BIOMERIT Research Centre, Microbiology Department, University College Cork, National University of Ireland, Cork, Ireland
Matthieu Barret
BIOMERIT Research Centre, Microbiology Department, University College Cork, National University of Ireland, Cork, Ireland
Pardraig M.Hegarty
BIOMERIT Research Centre, Microbiology Department, University College Cork, National University of Ireland, Cork, Ireland
Catriona Harrington
BIOMERIT Research Centre, Microbiology Department, University College Cork, National University of Ireland, Cork, Ireland
Alan D.W. Dobson
Environmental Research Institute, University College Cork, National University of Ireland, Cork, Ireland
Fergal O’Gara
Curtin University, School of Biomedical Sciences, Perth, WA, Australia
f.ogara@ucc.ie

Abstract
Environmental microorganisms are emerging as an important source of new enzymes for wide-scale industrial application. In this study, novel phytase genes were identified from a soil microbial community. For this, a function-based screening approach was utilized for the identification of phytase activity in a metagenomic library derived from an agricultural soil. Two novel phytases were identified. Interestingly, one of these phytases is an unusual histidine acid phosphatase family phytase, as the conserved motif of the active site of PhyX possesses an additional amino acid residue. The second phytase belongs to a new type, which is encoded by multiple open reading frames (ORFs) and is different to all phytases known to date, which are encoded by a single ORF.
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Acknowledgements
This research was supported in part by grants awarded by the Science Foundation of Ireland (07/IN.1/B948, 12/TIDA/B2411, 12/TIDA/B2405, 09/RFP/BMT 2350); the Department of Agriculture, Fisheries and Food (FIRM/RSF/CoFoRD; FIRM 08/RDC/629); the Environmental Protection Agency (EPA 2008-PhD/S-2), the Irish Research Council for Science, Engineering and Technology (PD/2011/2414; RS/2010/2413), the European Commission (FP7-PEOPLE-2013-ITN, 607786; OCEAN2012, 287589; FP7-KBBE-2012-6, 311975; FP7-KBBE-2012-6, CP-TP-312184; Marie Curie 256596); and the Marine Institute (Beaufort award C2CRA 2007/082); Teagasc (Walsh Fellowship 2013) and the Health Research Board (HRA/2009/146).
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