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Enhanced Production of <TEX>${\varepsilon}$</TEX>-Caprolactone by Coexpression of Bacterial Hemoglobin Gene in Recombinant Escherichia coli Expressing Cyclohexanone Monooxygenase Gene
Enhanced Production of ${\varepsilon}$-Caprolactone by Coexpression of Bacterial Hemoglobin Gene in Recombinant Escherichia coli Expressing Cyclohexanone Monooxygenase Gene
Won-Heong Lee , Eun-Hee Park , Myoung-Dong Kim
Journal of Microbiology and Biotechnology. 2014. Dec, 24(12): 1685-1689
DOI : http://dx.doi.org/10.4014/jmb.1409.09060
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : September 18, 2014
  • Accepted : September 29, 2014
  • Published : December 28, 2014
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Won-Heong Lee
Eun-Hee Park
Myoung-Dong Kim
About the Authors
Won-Heong Lee
Department of Bioenergy Science and Technology, Chonnam National University, Gwangju 500-757, Republic of Korea
Eun-Hee Park
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea
Myoung-Dong Kim
Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Republic of Korea
mdkim@kangwon.ac.kr
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Abstract
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Abstract
Baeyer-Villiger (BV) oxidation of cyclohexanone to ε-caprolactone in a microbial system expressing cyclohexanone monooxygenase (CHMO) can be influenced by not only the efficient regeneration of NADPH but also a sufficient supply of oxygen. In this study, the bacterial hemoglobin gene from Vitreoscilla stercoraria ( vhb ) was introduced into the recombinant Escherichia coli expressing CHMO to investigate the effects of an oxygen-carrying protein on microbial BV oxidation of cyclohexanone. Coexpression of Vhb allowed the recombinant E. coli strain to produce a maximum ε-caprolactone concentration of 15.7 g/l in a fed-batch BV oxidation of cyclohexanone, which corresponded to a 43% improvement compared with the control strain expressing CHMO only under the same conditions.
Keywords
Cyclohexanone monooxygenase ;  ε-caprolactone ;  bacterial hemoglobin ;  Escherichia coli
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Acknowledgements
This work was supported by “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ009993)”, Rural Development Administration, Republic of Korea.
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Citing 'Enhanced Production of ${\varepsilon}$-Caprolactone by Coexpression of Bacterial Hemoglobin Gene in Recombinant Escherichia coli Expressing Cyclohexanone Monooxygenase Gene '
@article{ E1MBA4_2014_v24n12_1685} ,title={Enhanced Production of ${\varepsilon}$-Caprolactone by Coexpression of Bacterial Hemoglobin Gene in Recombinant Escherichia coli Expressing Cyclohexanone Monooxygenase Gene} ,volume={12} , url={http://dx.doi.org/10.4014/jmb.1409.09060}, DOI={10.4014/jmb.1409.09060} , number= {12} , journal={Journal of Microbiology and Biotechnology} , publisher={The Korean Society for Applied Microbiology and Biotechnology} , author={Lee, Won-Heong and Park, Eun-Hee and Kim, Myoung-Dong} , year={2014} , month={Dec}