Oral Administration of Lactobacillus plantarum HY7714 Protects Hairless Mouse Against Ultraviolet B-Induced Photoaging
Oral Administration of Lactobacillus plantarum HY7714 Protects Hairless Mouse Against Ultraviolet B-Induced Photoaging
Journal of Microbiology and Biotechnology. 2014. Nov, 24(11): 1583-1591
Copyright © 2014, The Korean Society For Microbiology And Biotechnology
  • Received : June 13, 2014
  • Accepted : August 06, 2014
  • Published : November 28, 2014
Export by style
Cited by
About the Authors
Hyun Mee Kim
School of Biotechnology and Institute of Life Science and Resources, Kyunghee University, Yongin 449-701, Republic of Korea
Dong Eun Lee
Korea Yakult Co., Ltd., Yongin 446-901, Republic of Korea
Soo Dong Park
Korea Yakult Co., Ltd., Yongin 446-901, Republic of Korea
Yong-Tae Kim
Korea Yakult Co., Ltd., Yongin 446-901, Republic of Korea
Yu Jin Kim
Korea Yakult Co., Ltd., Yongin 446-901, Republic of Korea
Ji Woong Jeong
Korea Yakult Co., Ltd., Yongin 446-901, Republic of Korea
Sung Sik Jang
Korea Yakult Co., Ltd., Yongin 446-901, Republic of Korea
Young-Tae Ahn
Korea Yakult Co., Ltd., Yongin 446-901, Republic of Korea
Jae-Hun Sim
Korea Yakult Co., Ltd., Yongin 446-901, Republic of Korea
Chul-Sung Huh
Research Institute of Eco-friendly Animal Sciences, Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang 232-916, Republic of Korea
Dae Kyun Chung
RNA Inc., College of Life Science, Kyunghee University, Yongin 449-701, Republic of Korea
Jung-Hee Lee
Korea Yakult Co., Ltd., Yongin 446-901, Republic of Korea

Ultraviolet (UV) irradiation alters multiple molecular pathways in the skin, thereby inducing skin damage, including photoaging. In recent years, probiotics have gained interest due to their beneficial effects on skin health, such as inhibiting atopic dermatitis and improving skin immunity or inflammation. However, little is known about the effects of probiotics on UVB-induced photoaging. In this study, we evaluated the effect of Lactobacillus plantarum HY7714 against UVB-induced photoaging in human dermal fibroblasts and hairless mice. The results showed that L. plantarum HY7714 treatment effectively rescued UVB-reduced procollagen expression through the inhibition of UVB-induced matrix metalloproteinase (MMP)-1 expression in human dermal fibroblasts. Data from a western blot showed that L. plantarum HY7714 inhibited the phosphorylation of Jun N-terminal kinase, thereby suppressing the UVB-induced phosphorylation and expression of c-Jun. Oral administration of L. plantarum HY7714 clearly inhibited the number, depth, and area of wrinkles in hairless mouse skin. Histological data showed that L. plantarum HY7714 significantly inhibited UVB-induced epidermal thickness in mice. Western blot and zymography data also revealed that L. plantarum HY7714 effectively inhibited MMP-13 expression as well as MMP-2 and -9 activities in dermal tissue. Collectively, these results provide further insight regarding the skin biological actions of L. plantarum HY7714, a potential skin anti-photoaging agent.
Various environmental stressors, including smoking and ultraviolet (UV) radiation, induce skin damage, resulting in skin aging [31 , 36] . Out of the stressors that induce skin aging, direct exposure to UV radiation, especially its UVB component, is tightly related to a particular type of skin damage called photoaging, which is characterized by coarse and fine wrinkles, dryness, laxity, pigmentation, and increased skin thickness [11 , 20 , 24] .
Ultraviolet irradiation causes alterations in skin collagenous tissues caused by the breakdown of collagen, a major structural protein in the dermis. Collagen destruction by chronic sun exposure is one of the key factors contributing to the aged appearance of the skin. A large number of studies regarding UV-induced molecular mechanisms and signal transduction have been reported [1 , 36] .
The overriding factor in collagen loss is the activation of the collagenase matrix metalloproteinase (MMP)-1 by UVB exposure [25 , 30] . UVB triggers cellular signaling pathways through cell surface receptors or secondary messengers, such as reactive oxygen species, to phosphorylate kinases, including Jun N-terminal kinase (JNK), p38, and extracellular regulated kinases (ERKs) [8 , 15] . These kinases eventually stimulate transcriptional activities, thereby contributing to the regulation of target genes involved in skin photoaging, such as MMPs. Activator protein (AP)-1 is the major MMP transcriptional factor in UVB-induced skin. AP-1 is a homoor heterodimer of Jun or Fos in mammalian cells, and the activity or expression of each subunit is stimulated by an upstream kinase such as MAPKs [2 , 27] . MMPs are a family of zinc-dependent endopeptidases expressed in several cell types that degrade macromolecules from the extracellular matrix (ECM) [28] . Among these enzymes, MMP-1 (interstitial collagenase) plays an important role in the degradation of native types I and III collagen in human skin [25] . Once cleaved by MMP-1, collagen can be further broken down into small peptides by MMP-9, resulting in ECM degradation. Reduction in collagen fibers is an important mechanism involved in the development of skin aging [16] .
The role of probiotics in regulating intestinal health has been widely studied for over a century [9] . After ingestion, probiotics exert their biological effects through adhesion to intestinal epithelial cells, and contribute to a decrease in harmful bacteria and intestinal inflammation. Modulation of the intestinal microbiota is one of the important functions of probiotics, which is deeply associated with the modulation of the intestinal immune system, improving bowel movement and decreasing allergy [13 , 21] . However, in recent years, several lines of evidence suggest that some bacterial probiotics can modulate the skin immune system [3 , 12 , 31] . In human clinical trials, probiotic supplementation showed potential in the relief of atopic dermatitis and dry skin [7 , 10 , 14 , 26] . Dietary supplements containing a specific probiotic with several components from natural plants protected against the early damage induced by UV exposure, through regulating immune cells and inflammatory cytokines in humans [3] .In addition to protecting against cutaneous photo damage, it has been suggested that a specific strain of lactic acid bacteria showed anti-aging effects such as reduction in wrinkle formation and improved elasticity in hairless mice [31] . However, only a few studies were designed to determine the anti-photoaging effects and mechanisms of probiotics on UVB-irradiated skin. Thus, the present study was designed to investigate the anti-photoaging activity and molecular mechanisms of Lactobacillus plantarum HY7714 on UVB-irradiated skin, both in vitro and in vivo .
Materials and Methods
- Preparation of Bacteria for In Vitro and In Vivo Experiments
The four lactic acid bacterial strains used in the present study were isolated from healthy infant feces or breast milk. For the in vitro assay, these strains were inoculated in Man Rogosa Sharpe (MRS; BD; Sparks, MD, USA) broth, cultured at 37℃ for 24 h, harvested using centrifugation (1,500 × g , 10 min), washed twice with sterile phosphate-buffered saline (PBS), and resuspended to a final concentration of 10 10 CFU/ml. The bacteria were then heattreated (100℃, 15 min) and stored at -20℃ until use. For the in vivo assay, L. plantarum HY7714 was harvested as described above and resuspended at a final concentration of 1 × 10 9 CFU/ml in sterile PBS.
- Reagents
Antibodies against MMP-1, MMP-13, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibodies against phosphorylated extracellular regulated kinases (ERKs), total ERKs, phosphorylated p38, total p38, phosphorylated JNKs, total JNKs, phosphorylated c-Jun, and c-Jun were purchased from Cell Signaling Technology (Danvers, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).
- Cell Culture
Hs68 human dermal fibroblasts were purchased from the American Type Culture Collection (Manassas, VA, USA) and were cultured in monolayers at 37℃ in a 5% CO 2 incubator in Dulbecco’s modified Eagle’s medium containing 10% fetal bovine serum.
- UVB-Exposure of Hs68 Cells
Hs68 cells were seeded on a six-well plate at a density of 2 × 10 5 cells per well and grown in a culture medium for 24 h to reach 80% confluency. The cells were starved in serum-free medium for another 24 h and exposed to UVB at 50 mJ/cm 2 dose. The cells were then immediately treated with the test materials. UVB treatment was performed using Ultraviolet Crosslinkers (UVP; Upland, CA, USA) with a peak emission at 302 nm, and the distance from the UVB source to the plates was about 12.7 cm. Following irradiation, the cells were incubated for 15 min or 48 h and harvested for in vitro studies.
- Enzyme-Linked Immunosorbent Assay (ELISA)
Hs68 cells were cultured in a 24-well plate (5 × 10 4 cells/well) for 24 h. They were then treated with several lactic acid bacteria at 5 × 10 8 CFU/ml for 24 h. The cell culture medium was collected, and type I procollagen was quantified using a procollagen type I C-peptide enzyme immunoassay kit (Takara, Shiga, Japan).
- Western Blot Analysis
For in vitro western blotting, cells were grown to confluence in 6-well plates or 100 mm plates, washed two times with PBS, and lysed in radioimmunoprecipitation assay (RIPA) buffer. The protein concentration was determined using a DC assay kit (Bio-Rad Corp., Hercules, CA, USA). Lysate protein (10 µg) was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then electrophoretically transferred to a polyvinylidene difluoride membrane. After blotting, the membrane was incubated with the specific primary antibody at 4℃ overnight. Protein bands were visualized using a chemiluminescence detection kit after hybridization with a horseradish peroxidase-conjugated secondary antibody. For the in vivo test, to isolate proteins from the mouse skin, the dorsal skin of each mouse was excised and blended in RIPA buffer using a homogenizer. Proteins were analyzed as described above for the in vitro western blot assay. The relative amounts of proteins associated with specific antibodies were quantified using Image J software (National Institutes of Health, Bethesda, MD, USA).
- Animals and Experimental Design
Five-week-old female hairless mice were purchased from Central Lab Animal Inc. (Seoul, Korea). The mice were maintained in climate-controlled quarters (at 24℃, 50% humidity) with a 12 h light/12 h dark cycle. The animal protocol used in this study was reviewed and approved based on ethical procedures and scientific care by the Ethics Committee at the R&BD Center of the Korea Yakult Company Limited (KYIACUC-11-04-0231-Y). The mice were divided into a control group ( n = 8), a UVB-only treatment group ( n = 8), and a UVB plus L. plantarum HY7714 treatment group ( n = 8). The mice in the control and UVB-only treatment groups were orally administered 100 µl of PB S. The mice in the UVB plus L. plantarum HY7714 treatment group were orally administered 100 µl of PBS containing 1 × 10 9 CFU of L. plantarum HY7714/mouse daily, 1 h prior to UVB irradiation.
- Mouse UVB Irradiation
The distance from the UV source to the skin was about 12.7 cm, irradiation and performed using Ultraviolet Crosslinkers (UVP; Upland, CA, USA) with a peak emission at 302 nm. UVB radiation was applied to the back of the mice three times per week for 12 weeks. The starting dose of UVB radiation was 25 mJ/cm 2 (1 minimal erythematous dose [MED]) during the first week, and the dose was increased weekly by 1 MED (25 mJ/cm 2 ) until reaching 4 MED (100 mJ/cm 2 ), which was maintained until 12 weeks. Body weights were recorded weekly. Replica preparation was performed at the 12th week of radiation exposure.
- Production of Replicas and Image Analysis
Replicas of mouse dorsal skin were constructed using SILFLO (CuDerm Corporation, Dallas, TX, USA). The impression replicas were set on a horizontal sample stand, and wrinkle images were obtained using a CCD camera and analyzed with Skin VisioLine 650 software (Courage & Khazaka Electronic GmbH, Cologne, Germany).
- Gelatin Zymography
The dorsal skin of each mouse was blended in RIPA buffer using a homogenizer, and equal amounts of the protein extract were then mixed with non-reducing sample buffer, incubated for 15 min at room temperature, and then resolved on 12% SDS-PAGE gels containing 1 mg/ml gelatin. The gels were washed twice with 2.5% Triton X-100 for 30 min, rinsed three times for 30 min with a 50 mM Tris-HCl buffer (pH 7.6) containing 5 mM CaCl 2 , 0.02% Brij-35, and 0.2% sodium azide, and incubated overnight at 37℃. The gels were then stained with a 0.5% Coomassie brilliant blue R-250 solution containing 10% acetic acid and 20% methanol for 30 min, and were destained with a 7.5% acetic acid solution containing 10% methanol. Areas of gelatinase activity were detected as clear bands against the blue-stained gelatin background. Gelatinase activity was quantified by densitometric analysis of the clear bands (as scanned JPEG images) using Image J software (NIH).
- Statistical Analysis
Where appropriate, data are expressed as means ± SD values, and the Student’s t -test was used for multiple statistical comparisons. A probability value of p < 0.05 was used as the criterion for statistical significance.
- Lactic Acid Bacteria Increase Procollagen Production in Hs68 Cells
Decrease in type I collagen, the primary component of the ECM, which supports the skin dermis, is a main cause for photoaging [36] . Thus, we first examined the activity of various probiotics isolated from healthy infant feces or breast milk on procollagen production in Hs68 cells to select the probiotic that presented the strongest anti-photoaging activity. Among the probiotics tested, L. plantarum HY7714 potently increased procollagen secretion by 32% in the cells, whereas other probiotics had no significant effect ( Fig. 1 ). All of the probiotics tested showed no cytotoxic activities with 10-fold more concentration in the Hs68 cell line (data not shown).
PPT Slide
Lager Image
Effects of several probiotics on procollagen expression in Hs68 cells. The cells were treated with several probiotics at 1 × 109 CFU/ml for 24 h. The culture supernatants were harvested, and type I procollagen protein levels in the supernatants were determined by ELISA. Data are representative of three independent experiments. LP 7714, L. plantarum HY7714; LR 24, L. rhamnosus 24; LR 25, L. rhamnosus 25; LP 26, L. plantarum 26. Asterisks (*) indicate significant differences (p < 0.05) between the control group and the probiotics-treated groups.
- L. plantarum HY7714 Rescues UVB-Reduced Procollagen Expression and Suppresses UVB-Induced MMP-1 Expression in Hs68 Cells
UV radiation degrades collagen fibers and causes the collapse of the dermal structure, followed by an acceleration of the aging process. Because the induction of interstitial collagenase (MMP-1) by UVB exposure is a prominent factor in the loss of collagen fibers, resulting in coarse wrinkles, MMP-1 has been considered as the primary marker for skin photoaging [5] . Thus, to evaluate the effects of L. plantarum HY7714 on procollagen or MMP-1 expression in UVB-irradiated dermal fibroblasts, we performed western blot analysis. The result showed that UVB irradiation clearly inhibited procollagen expression in Hs68 cells, and that L. plantarum HY7714 effectively rescued procollagen expression in a dose-dependent manner ( Fig. 2 A). UVB exposure also induced a 2-fold increase in MMP-1 expression compared with that of normal cells. In contrast, L. plantarum HY7714 notably suppressed UVB-induced MMP-1 expression in a dose-dependent manner ( Fig. 2 B).
PPT Slide
Lager Image
Effect of L. plantarum HY7714 on UVB-reduced procollagen expression or UVB-induced MMP-1 expression in Hs68 cells. The cells were treated with L. plantarum HY7714 at 5 × 108 CFU/ml or 1 × 109 CFU/ml, and simultaneously exposed to UVB (50 mJ/cm2) for 48 h. The level of procollagen or MMP-1 was determined by western blot analysis. Data are representative of three independent experiments. The protein levels were quantified using an image analysis program to evaluate the density of each band on the immunoblot, and the fold-change value was calculated.
- L. plantarum HY7714 Suppresses UVB-Induced Phosphorylation of JNK/c-Jun, but Not ERK and p38, in Hs68 Cells
Numerous reports suggested that UVB irradiation activates MAPKs, including ERK, JNK, or p38, resulting in the induction of MMP-1 expression in dermal cells. Therefore, to elucidate the mechanisms involved in the effects of L. plantarum HY7714 on UVB-induced MMP-1 expression, we examined whether L. plantarum HY7714 blocked the activation of the ERK, JNK, or p38 pathways stimulated by UVB in Hs68 cells. Western blotting data showed that L. plantarum HY7714 considerably inhibited UVB-induced phosphorylation of JNK at doses of 5 × 10 7 CFU/ml and 5 × 10 8 CFU/ml. On the other hand, L. plantarum HY7714 had no effect on the phosphorylation of p38 expression, and the phosphorylation of ERKs was rather gradually increased dose dependently ( Fig. 3 A). These results indicate that the inhibition of UVB-induced MMP-1 expression by L. plantarum HY7714 was mainly caused by the inhibition of JNK phosphorylation. MMP-1 expression is strongly regulated by the transcription factor AP-1, which is rapidly induced and activated by UV exposure in human skin in vivo . To examine this mechanism, we evaluated the expression and the phosphorylation of AP-1 subunits. The results showed that c-Jun phosphorylation and expression were significantly diminished after treatment with L. plantarum HY7714 in Hs68 cells. However, L. plantarum HY7714 did not inhibit c-Fos expression (data not shown).
PPT Slide
Lager Image
Effect of L. plantarum HY7714 on UVB-induced signaling pathway in Hs68 cells. (A) Effect of L. plantarum HY7714 on UVB-induced MAPK phosphorylation in Hs68 cells. (B) Effect of L. plantarum HY7714 on UVB-induced c-Jun phosphorylation or expression in Hs68 cells. For A and B, the cells were co-treated with L. plantarum HY7714 at 5 × 108 CFU/ml or 1 × 109 CFU/ml and UVB (50 mJ/cm2) for 15 min. Western blotting was conducted using the indicated specific antibodies. Data are representative of three independent experiments.
- L. plantarum HY7714 Attenuates UVB-Induced Skin Wrinkle Formation in Hairless Mouse Skin
In order to confirm the anti-photoaging activity of L. plantarum HY7714 in vivo , we orally administered L. plantarum HY7714 at a dose of 1 × 10 9 CFU in 100 µl of sterile saline, or vehicle alone, to hairless mice for 12 weeks. In the UVB-only group, diamond-shaped thick and deep wrinkles were observed on the dorsal skin of the mice. In contrast, oral administration of L. plantarum HY7714 strongly reduced the wrinkle formation. To quantitatively analyze the number, area, and depth of wrinkles, we obtained the replicas from mouse dorsal skin at 12 weeks after UVB irradiation. The skin replicas were photographed and the three parameters were estimated using the skin VisioLine 650 system. These photograph data showed that wrinkle formation was alleviated by oral administration of L. plantarum HY7714 prior to UVB irradiation ( Fig. 4 A). The number of wrinkles in mice from the UVB-only-treated group was increased by 2.5-fold compared with that of mice from the control group. However, L. plantarum HY7714 administration inhibited wrinkle numbers by 56% ( Fig. 4 B). Moreover, the total area and depth of wrinkles was suppressed by 48% and 83%, respectively, compared with that of the UVB-only-treated group ( Figs. 4 C and 4 D).
PPT Slide
Lager Image
Effect of oral administration of L. plantarum HY7714 on UVB-induced wrinkle formation in hairless mouse skin. (A) Photomicrographs of representative replicas taken from the dorsal mouse skin at the end of the study (×100). Number (B), total area (C), or total depth (D) of wrinkles calculated from the replica by image analysis. Results are presented as the mean ± SD of eight mice per group. The hash symbol indicates significant differences in wrinkle formation between the control group and the group exposed to UVB (##, p < 0.01). The asterisks indicate a significant decrease in wrinkle formation between the group treated with UVB alone and the group treated with L. plantarum HY7714 (**, p < 0.01; *, p < 0.05).
- L. plantarum HY7714 Inhibits UVB-Induced Epidermal Hyperplasia in Hairless Mice Skin
Since UVB irradiation induces the epidermis to become significantly thicker, we further evaluated the effect of L. plantarum HY7714 on UVB-induced epidermal thickening. To measure the thickness of the epidermis, biopsied skin specimens were stained with hematoxylin and eosin. The results showed that UVB-induced epidermal thickness of the dorsal skin was dramatically increased by 2.3-fold, due to chronic UVB exposure. On the other hand, oral administration of L. plantarum HY7714 strongly inhibited UVB-induced epidermal thickness by 65% ( p < 0.01 vs . UVB-only treated group) ( Fig. 5 ).
PPT Slide
Lager Image
Effect of oral administration of L. plantarum HY7714 on UVB-induced epidermal thickening in hairless mouse skin. (A) Hematoxylin and eosin-stained skin tissue sections. Images are representative of results from eight tissue samples (×100). (B) Bars represent the mean thickness (µm) of the epidermis from eight animals (three measurements/section). Results are presented as means ± SD (n = 8). The ## indicates significant differences at p < 0.01 between the control group and the group exposed to UVB. The ** indicates significant differences at p < 0.01 between the group treated with UVB alone and the group treated with L. plantarum HY7714.
- L. plantarum HY7714 Inhibits UVB-Induced Activity or Expression of MMPs in Hairless Mouse Skin
MMP-13, but not MMP-1, functions as a collagenase in mice, and is primarily induced in the epidermis before diffusing into the dermis and degrading collagen. To understand how L. plantarum HY7714 suppressed UVBinduced wrinkle formation, MMP expression and activities were examined using western blotting and zymography. In this study, repetitive UVB irradiation on mouse dorsal skin induced MMP-13 expression, and L. plantarum HY7714 administration significantly attenuated it ( Fig. 6 A). Because gelatinase, MMP-2, and MMP-9 degrade the interstitial collagen and basement, which results in skin wrinkling [18] , we next evaluated whether L. plantarum HY7714 could affect UVB-induced MMP-2 and MMP-9 activities. Zymography results showed that oral administration of L. plantarum HY7714 effectively inhibited MMP-2 and MMP-9 activities in hairless mouse skin ( Fig. 6 B). Therefore, these results indicated that L. plantarum HY7714 could improve skin wrinkles by reducing collagen-degrading MMP expression or activities.
PPT Slide
Lager Image
Effect of oral administration of L. plantarum HY7714 on UVB-induced MMP-13 expression and MMP-2/-9 activities in hairless mouse skin. (A) MMP-13 protein levels were determined by western blot analysis. (B) MMP-2/-9 activities were determined by zymography in mouse skin. The protein levels were quantified using an image analysis program to evaluate the density of each band on the immunoblot or zymography, and the fold-change value was calculated. Data are representative of three independent experiments. Results are presented as means ± SD (n = 3). High symbols (# and ##) indicate a significant difference (p < 0.05 and p < 0.01, respectively) between the control group and the group exposed to UVB. Asterisks (* and **) indicate a significant difference (p < 0.05 and p < 0.01, respectively) between the group exposed to UVB and the group treated with L. plantarum HY7714.
There is growing interest in the dermal benefits of probiotics, such as for inhibiting atopic dermatitis [14 , 29] and atopic eczema [6 , 19] and improving skin immunity [3 , 12] . In addition, recent studies revealed that oral administration of some probiotics can suppress skin damage through inhibiting skin inflammation and improving wrinkle formation or skin elasticity in mice [31] . These activities are diverse and depend on different strains. Although identification of the bioactive components of probiotics are very important to better understand their physiological functions on human health, this area of research remains poorly understood and needs to be investigated further. There is increasing evidence that cell wall components of lactic acid bacteria can inhibit MMP-1 expression in fibroblasts [37] or skin carcinogenesis in hairless mice [35] . The metabolites and heat-treated bacteria from probiotics can also cause certain immune responses in the skin and improve atopic dermatitis in the NC/Nga mouse model [29 , 32 , 34] . In this study, we used live bacteria in the hairless mouse model. Thus, further studies are necessary to identify the probiotic components such as lipoteichoic acid or other metabolites responsible for the biological functions observed.
Numerous studies have shown that the MAPK signal transduction pathways are responsible for regulating a variety of cellular functions, such as cell inflammation, MMP expression, and type I collagen synthesis [38]. Among these, JNKs and p38 are generally considered as stressactivated MAPKs [5] . Several MMPs, especially MMP-1, MMP-2, and MMP-9, are known to be activated by AP-1. Upon UV irradiation of human skin, c-Jun protein is activated and combines with c-Fos to form an active form of the transcription factor AP-1 [17] . This UV-induced AP-1 initiates the transcription of MMP genes, encoding collagenase and gelatinase in both keratinocytes and fibroblasts [23 , 33] . In particular, the protein c-Jun is a well-known nuclear substrate of JNK1, and c-Jun is an important component of various AP-1 subunits that bind to AP-1/ 12-O-tetradecanoyl-13-phorbol-acetate-responsive element enhancer elements in gene regulatory sites [22] . In this study, the JNK/c-Jun pathway was markedly increased by UVB, and L. plantarum HY7714 treatment subsequently prevented this JNK/AP-1 activation.
Collagen is the major structural component of the dermal tissue, and its degradation and degenerative changes cause wrinkle formation and aging appearance [4] . UV irradiation increased MMP expression in human skin, including MMP-1, MMP-2, and MMP-9 [16] . MMP-1 stimulates the cleavage of fibrillar collagen, and MMP-9 further degrades the cleaved collagen [15] . The gelatinases MMP-2 and MMP-9 can specifically degrade components of the epidermal basement membrane such as types IV and VII collagen [16] . It was reported that instant UV stimulation of the dermal tissue only increased MMP-9 levels, whereas constant UV exposure caused increases of both MMP-2 and MMP-9 levels in hairless mice [16] . Thus, MMP-2 as well as MMP-9 may play an important role in the photoaging process. Collagen-degrading MMP-2, -9, and -13 are primarily induced in the epidermis by UV irradiation, as shown by zymography ( Fig. 6 ). Specifically, MMP-2 is highly expressed in the hairless mouse epidermis. These results show that MMP-2 might be one of the important markers of chronically induced photoaging.
In the present study, we found that L. plantarum HY7714 had a strong inhibitory effect on UVB-induced MMP-1 expression by inhibiting the JNK/c-Jun pathway in Hs68 cells. Oral administration of L. plantarum HY7714 also suppressed UVB-induced MMP-13 expression and MMP-2 and MMP-9 activities, thereby inhibiting wrinkle formation and epidermal thickening in the dorsal skin of hairless mice. These results demonstrate that L. plantarum HY7714 might be a good candidate as a skin anti-photoaging agent. However, the detailed molecular mechanisms and bioactive compounds responsible for the increased anti-photoaging effects of L. plantarum HY7714 remain to be elucidated.
This work was supported by the Interrelated Development Program (R-0000452) of Inter-Economic Regions, Ministry of Trade, Industry & Energy, Republic of Korea.
View Fulltext  
Amano S 2009 Possible involvement of basement membrane damage in skin photoaging. J. Investig. Dermatol. Symp. Proc. 14 2 - 7    DOI : 10.1038/jidsymp.2009.5
Berneburg M , Plettenberg H , Krutmann J 2000 Photoaging of human skin. Photodermatol. Photoimmunol. Photomed. 16 239 - 244    DOI : 10.1034/j.1600-0781.2000.160601.x
Bouilly-Gauthier D , Jeannes C , Maubert Y , Duteil L , Queille-Roussel C , Piccardi N 2010 Clinical evidence of benefits of a dietary supplement containing probiotic and carotenoids on ultraviolet-induced skin damage. Br. J. Dermatol. 163 536 - 543    DOI : 10.1111/j.1365-2133.2010.09888.x
Chung JH , Seo JY , Choi HR , Lee MK , Youn CS , Rhie G 2001 Modulation of skin collagen metabolism in aged and photoaged human skinin vivo. J. Invest. Dermatol. 117 1218 - 1224    DOI : 10.1046/j.0022-202x.2001.01544.x
Cuenda A 2000 Mitogen-activated protein kinase kinase 4 (MKK4). Int. J. Biochem. Cell Biol. 32 581 - 587    DOI : 10.1016/S1357-2725(00)00003-0
Eigenmann PA 2013 Evidence of preventive effect of probiotics and prebiotics for infantile eczema. Curr. Opin. Allergy Clin. Immunol. 13 426 - 431
Elazab N , Mendy A , Gasana J , Vieira ER , Quizon A , Forno E 2013 Probiotic administration in early life, atopy, and asthma: a meta-analysis of clinical trials. Pediatrics 132 e666 - e676    DOI : 10.1542/peds.2013-0246
Fisher G , Voorhees JJ 1998 Molecular mechanisms of photoaging and its prevention by retinoic acid: ultraviolet irradiation induces MAP kinase signal transduction cascades that induce Ap-1-regulated matrix metalloproteinases that degrade human skinin vivo. J. Investig. Dermatol. Symp. Proc. 3 61 - 68
Foligné B , Daniel C , Pot B 2013 Probiotics from research to market: the possibilities, risks and challenges. Curr. Opin. Microbiol. 16 284 - 292    DOI : 10.1016/j.mib.2013.06.008
Foolad N , Brezinski EA , Chase EP , Armstrong AW 2013 Effect of nutrient supplementation on atopic dermatitis in children: a systematic review of probiotics, prebiotics, formula, and fatty acids. JAMA Dermatol. 149 350 - 355    DOI : 10.1001/jamadermatol.2013.1495
Grether-Beck S , Marini A , Jaenicke T , Krutmann J 2014 Photoprotection of human skin beyond ultraviolet radiation. Photodermatol. Photoimmunol. Photomed. 30 167 - 174    DOI : 10.1111/phpp.12111
Guéniche A , Philippe D , Bastien P , Blum S , Buyukpamukcu E , Castiel-Higounenc I 2009 Probiotics for photoprotection. Dermatoendocrinology 1 275 - 279    DOI : 10.4161/derm.1.5.9849
Guéniche A , Bastien P , Ovigne JM , Kermici M , Courchay G , Chevalier V 2010 Bifidobacterium longumlysate, a new ingredient for reactive skin. Exp. Dermatol. 19 e1 - e8    DOI : 10.1111/j.1600-0625.2009.00932.x
Han Y , Kim B , Ban J , Lee J , Kim BJ , Choi BS 2012 A randomized trial ofLactobacillus plantarumCJLP133 for the treatment of atopic dermatitis. Pediatr. Allergy Immunol. 23 667 - 673    DOI : 10.1111/pai.12010
Hwang BM , Noh EM , Kim JS , Kim JM , You YO , Hwang JK 2013 Curcumin inhibits UVB-induced matrix metalloproteinase-1/3 expression by suppressing the MAPK-p38/JNK pathways in human dermal fibroblasts. Exp. Dermatol. 22 371 - 374    DOI : 10.1111/exd.12137
Inomata S , Matsunaga Y , Amano S , Takada K , Kobayashi K , Tsunenaga M 2003 Possible involvement of gelatinases in basement membrane damage and wrinkle formation in chronically ultraviolet B-exposed hairless mouse. J. Invest. Dermatol. 120 128 - 134    DOI : 10.1046/j.1523-1747.2003.12021.x
Janulis M , Silberman S , Ambegaokar A , Gutkind JS , Schultz RM 1999 Role of mitogen-activated protein kinases and cJun/AP-1 trans-activating activity in the regulation of protease mRNAs and the malignant phenotype in NIH 3T3 fibroblasts J. Biol. Chem. 274 801 - 813    DOI : 10.1074/jbc.274.2.801
Kim JA , Ahn BN , Kong CS , Kim SK 2012 Protective effect of chromene isolated fromSargassum horneriagainst UV-A-induced damage in skin dermal fibroblasts. Exp. Dermatol. 21 630 - 631    DOI : 10.1111/j.1600-0625.2012.01535.x
Kuitunen M 2013 Probiotics and Rebiotics in preventing food allergy and eczema. Curr. Opin. Allergy Clin. Immunol. 13 280 - 286
Lee KE , Mun S , Pyun HB , Kim MS , Hwang JK 2012 Effects of macelignan isolated fromMyristica fragrans(Nutmeg) on expression of matrix metalloproteinase-1 and type I procollagen in UVB-irradiated human skin fibroblasts. Biol. Pharm. Bull. 35 1669 - 1675    DOI : 10.1248/bpb.b12-00037
Lew LC , Liong MT 2013 Bioactives from probiotics for dermal health: functions and benefits. J. Appl. Microbiol. 114 1241 - 1253    DOI : 10.1111/jam.12137
Meng Q , Xia Y 2011 c-Jun, at the crossroad of the signaling network. Protein Cell 2 889 - 898    DOI : 10.1007/s13238-011-1113-3
Poulalhon N , Farge D , Roos N , Tacheau C , Neuzillet C , Michel L 2006 Modulation of collagen and MMP-1 gene expression in fibroblasts by the immunosuppressive drug rapamycin. A direct role as an antifibrotic agent. J. Biol. Chem. 281 33045 - 33052    DOI : 10.1074/jbc.M606366200
Pyun HB , Kim M , Park J , Sakai Y , Numata N , Shin JY 2012 Effects of collagen tripeptide supplement on photoaging and epidermal skin barrier in UVB-exposed hairless mice. Prev. Nutr. Food Sci. 17 245 - 253    DOI : 10.3746/pnf.2012.17.4.245
Quan T , Qin Z , Xia W , Shao Y , Voorhees JJ , Fisher GJ 2009 Matrix-degrading metalloproteinases in photoaging. J. Investig. Dermatol. Symp. Proc. 14 20 - 24    DOI : 10.1038/jidsymp.2009.8
Raone B , Raboni R , Patrizi A 2013 Probiotics reduce gut microbial translocation and improve adult atopic dermatitis. J. Clin. Gastroenterol. 48 95 - 96    DOI : 10.1097/MCG.0b013e31829e4632
Rittié L , Fisher GJ 2002 UV-light-induced signal cascades and skin aging. Ageing Res. Rev. 1 705 - 720    DOI : 10.1016/S1568-1637(02)00024-7
Ropke CD , da Silva VV , Kera CZ , Miranda DV , de Almeida RL , Sawada TC , Barros SB 2006 In vitroandin vivoinhibition of skin matrix metalloproteinases byPothomorphe umbellataroot extract. Photochem. Photobiol. 82 439 - 442    DOI : 10.1562/2005-06-29-RA-596
Sawada J , Morita H , Tanaka A , Salminen S , He F , Matsuda H 2007 Ingestion of heat-treatedLactobacillus rhamnosusGG prevents development of atopic dermatitis in NC/Nga mice. Clin. Exp. Immunol. 37 296 - 303
Staniforth V , Huang WC , Aravindaram K , Yang NS 2012 Ferulic acid, a phenolic phytochemical, inhibits UVB-induced matrix metalloproteinases in mouse skinviaposttranslational mechanisms. J. Nutr. Biochem. 23 443 - 451    DOI : 10.1016/j.jnutbio.2011.01.009
Sugimoto S , Ishii Y , Izawa N , Masuoka N , Kano M , Sone T 2012 Photoprotective effects ofBifidobacterium brevesupplementation against skin damage induced by ultraviolet irradiation in hairless mice. Photodermatol. Photoimmunol. Photomed. 28 312 - 319    DOI : 10.1111/phpp.12006
Tobita K , Yanaka H , Otani H 2009 Heat-treatedLactobacillus crispatusKT strains reduce allergic symptoms in mice. J. Agric. Food Chem. 57 5586 - 5590    DOI : 10.1021/jf900703q
Vincenti MP , White LA , Schroen DJ , Benbow U , Brinckerhoff CE 1996 Regulating expression of the gene for matrix metalloproteinase-1 (collagenase): mechanisms that control enzyme activity, transcription, and mRNA stability. Crit. Rev. Eukaryot. Gene Expr. 6 391 - 411    DOI : 10.1615/CritRevEukarGeneExpr.v6.i4.40
Watanabe T , Hamada K , Tategaki A , Kishida H , Tanaka H , Kitano M , Miyamoto T 2009 Oral administration of lactic acid bacteria isolated from traditional South Asian fermented milk ‘dahi’ inhibits the development of atopic dermatitis in NC/Nga mice. J. Nutr. Sci. Vitaminol. 55 271 - 278    DOI : 10.3177/jnsv.55.271
Weill FS , Cela EM , Paz ML , Ferrari A , Leoni J , Maglio DH 2013 Lipoteichoic acid fromLactobacillus rhamnosusGG as an oral photoprotective agent against UV-induced carcinogenesis. Br. J. Nutr. 109 457 - 466    DOI : 10.1017/S0007114512001225
Yu BC , Lee DS , Bae SM , Jung WK , Chun JH , Urm SH 2013 The effect of cilostazol on the expression of matrix metalloproteinase-1 and type I procollagen in ultravioletirradiated human dermal fibroblasts. Life Sci. 92 282 - 288    DOI : 10.1016/j.lfs.2012.12.011
You GE , Jung BJ , Kim HR , Kim HG , Kim TR , Chung DK 2013 Lactobacillus sakeilipoteichoic acid inhibits MMP-1 induced by UVA in normal dermal fibroblasts of human. J. Microbiol. Biotechnol. 23 1357 - 1364    DOI : 10.4014/jmb.1306.06026